Localization and partial characterization of thermostable glucoamylase produced by newly isolated Thermomyces lanuginosus TO3 in submerged fermentation

Abstract

Thermophilic Thermomyces lanuginosus strain TO3 was isolated from compost pile samples and was used for its ability to produce considerable glucoamylase activity when growing in liquid medium at 45ºC with starch as the sole carbon source. Enzyme productivity was high in submerged fermentation (SmF) with maximum activity of 13 U/mL after 168 h of fermentation. Higher quantities of glucose were released when the substrate for enzyme was soluble starch than maltose or maltooligosaccharides were used. The distribution of glucoamylase between the extracellular and cell-associated fractions varied according to fermentation time. Glucoamylase produced from T. lanuginosus TO3 had optimum activity at 65 ºC and good thermostability in the absence of substrate, with a half-life of 6 h at 60 ºC. The enzyme was stable over a wide pH range (4.0-10.0).<br>O fungo termofílico Thermomyces lanuginosus TO3 foi isolado a partir de amostras de material de pilhas de compostagem, com base em sua capacidade de crescer em meio líquido contendo amido como única fonte de carbono, a 45 ºC, e produzir considerável quantidade de glucoamilase. A produção da enzima por fermentação submersa FSm foi alta, com um máximo de 13 U/mL em 168 h de fermentação. A atividade enzimática foi maior sobre amido do que sobre a maltose e maltooligosacarideos. As atividades de glucoamilase extra e intracelular variaram com o tempo de fermentação. A glucoamilase produzidas por T. lanuginosus TO3 apresentou elevada temperatura ótima de atividade (65 -70 ºC) com boa termoestabilidade em ausência de substrato, apresentando uma meia vida de 6 h a 60ºC, além de estabilidade em ampla faixa de pH. Os resultados apresentados indicam uma importante fonte alternativa de glucoamilase para uso no processamento industrial de amido

Sequential Statistical Optimization of Media Components for the Production of Glucoamylase by Thermophilic Fungus Humicola grisea MTCC 352

“…This optimization strategy led to the enhancement of glucoamylase production from 4.57 U/mL (unoptimized medium) to 12.27 U/mL (optimized medium), a 2.68-fold increase. In addition to this, the optimized glucoamylase activity was found to be higher than the available literature value for various thermophilic fungi such as Scytalidium thermophilum 15.8 (3.62 U/mL) [ 26 ], Thermomyces lanuginosus A.13.37 (2.8 U/mL) [ 2 ], and Thermomyces lanuginosus ATCC 200065 (7.4 U/mL) [ 27 ] and comparable with Thermomyces lanuginosus TO3 (13 U/mL) [ 28 ].…”

Thermostable xylanase from thermophilic fungi: Biochemical properties and industrial applications

“…lanuginosus is an interesting fungus because it can produce enzymes of industrial interest such as invertase (Chaudhuri and Maheshwari, 1996), β-xylosidase (Corrêa et al, 2016), chitinase (Khan et al, 2015;Chen et al, 2012;Zhang et al, 2015), protease (Li et al, 1997), inulinase (FloresGallegos et al, 2015), esterase , amylase (Kunamneni et al, 2005), glucoamylase (Gonçalves et al, 2008), α-galactosidase (Rezessy-Szabó et al, 2007), and the two most reported enzymes, lipase Ávila-Cisneros et al, 2014;Fang et al, 2014) and xylanase Stephens et al, 2014;Shrivastava et al, 2013;Manimaran et al, 2009). Furthermore, T. lanuginosus is known for producing cellulase-free xylanases (Manimaran et al, 2009;Li et al, 2005;Damaso et al, 2002).…”