Cloning and characterization of the iron uptake gene iutA from avian Escherichia coli

Tokano, Dorismey Vieira; Kawaichi, Marisa Emiko; Venâncio, Emerson José; Vidotto, Marilda Carlos

doi:10.1590/s1516-89132008000300006

Cited by 8 publications

(5 citation statements)

References 27 publications

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“…In this context, Crecente et al engineered polymeric nanocapsules (NCs) to deliver the IutA antigen. IutA is an aerobactin receptor associated to UPEC iron uptake (Tokano et al, 2008), which was included in the NC to achieve an early presentation of the antigen to the organism, enhancing antibody production. In this NCs, the antigen IutA was entrapped between CS and dextran sulfate layers.…”

Section: Antibacterialmentioning

confidence: 99%

Nanoparticles as Potential Novel Therapies for Urinary Tract Infections

Sánchez

Navarro

Catalán-Figueroa

et al. 2021

Front. Cell. Infect. Microbiol.

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Urinary tract infection (UTI) is one of the most common reasons for antibiotic treatment. Nevertheless, uropathogens are steadily becoming resistant to currently available therapies. In this context, nanotechnology emerges as an innovative and promising approach among diverse strategies currently under development. In this review we deeply discuss different nanoparticles (NPs) used in UTI treatment, including organic NPs, nanodiamonds, chemical and green synthesized inorganic NPs, and NPs made of composite materials. In addition, we compare the effects of different NPs against uropathogens in vivo and in vitro and discuss their potential impact the in the near future.

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Section: Antibacterialmentioning

confidence: 99%

Nanoparticles as Potential Novel Therapies for Urinary Tract Infections

Sánchez

Navarro

Catalán-Figueroa

et al. 2021

Front. Cell. Infect. Microbiol.

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“…This prevalence signifies that E.coli organisms in the study context are more likely to carry virulence and integron genes to their plasmids. The existence of virulence genes of iutA, fimA, and neuC in E.coli are even cited in some studies [20,21,22], which may contribute to their rising AR. These are believed to be the important virulence factors in many infections including RTIs caused by E.coli [23].…”

Section: Plasmid Profiling Of Ecoli Isolatesmentioning

confidence: 99%

Emerging Antibiotic Resistance and Plasmid DNA in Escherichia coli Isolated from Respiratory Tract Infections in Bangladesh

Saha¹

2017

OAJTMR

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The study aimed to investigate antibiotic resistance pattern and presence of plasmid DNA among Escherichia coli isolates from respiratory tract infections (RTIs). A total of 32 E.coli isolates were characterized by using selective media and biochemical tests. Kirby-Bauer disc diffusion method was used for antibiotic sensitivity testing. Plasmid DNA extraction was carried out using Bimboim and Doly method and detection was confirmed by agarose gel electrophoresis. Overall, 51% antibiotics were resistant and 46% were sensitive out of 20 tested antibiotics. Cephalosporin antibiotics (4/7) were highly resistant (76%). Amoxicillin showed 100% resistance. The higher rate of resistance was observed for cefuroxime, ceftazidime, cephalexin, cephradine, gentamycin and nalidixic acid ranges with 75-80%. E.coli showed better sensitivity to nitrofurantoin (78%), levofloxacin (89%) and amikacin (100%). Amoxiclav, cefotaxime, ciprofloxacin and piperacillin/tazobactam antibiotics were sensitive to nearly 40% E.coli. 100% sensitivity observed for imipenem, meropenem, colistin, and amikacin. The plasmid profiling revealed that 92% E.coli harbored large plasmid DNA which justifies their emerging resistance to most of the antibiotics. This study concludes that nitrofurantoin, levofloxacin, cefotaxime, amoxiclav and amikacin antibiotics may be wise to prescribe to treat RTIs caused by E.coli. Further studies are recommended to explore the association between virulence factors and AR in E.coli isolated from RTIs.

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“…Many Escherichia coli strains contain the astA gene, which codes for the enteroaggregative E. coli heat-stable enterotoxin (EAST1) (Maluta et al 2017). Though the aerobactin receptor (IutA protein) is an important virulence factor in extra-intestinal infections, little research has been carried out as a protective antigen (Tokano et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Risk Factors, Antibiotic Profile, and Molecular Detection of Virulence and Antibiotic Resistance Genes of Enteric Bacteria in Diarrheic Calves in Egypt

2022

Int J Vet Sci

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Diarrhea caused by different bacterial infections in calves is a serious issue. In the present study, 215 diarrheic animals, including 175 buffalo and 40 cattle calves were examined. The incidence varied amongst farms, ranging from 0 to 27.9%. Among the affected calves, 37.2% were 1 to 7 days old, 55.8% were 7 days to 3 months of age, while 6.9% were more than 3 months old. Bacteriological, antibiogram, and PCR-based detection of specific virulence and antimicrobial resistance genes were performed. E. coli (85.5%), C. perfringens (8.8%), and Salmonella (3.7%) were bacterial infections recovered from affected calves. Various E. coli pathotypes, such as Shiga-toxin-producing E. coli (STEC), enteropathogenic E. coli (EPEC), extraintestinal E. coli (ExPEC), and enterotoxigenic E. coli (ETEC), caused diarrhea in these calves. The most prevalent virulence genes in E. coli were the sta and eaeA genes (60%). All Salmonella strains were found positive for the invA gene, while all C. perfringens strains were tested positive for the cpa gene. Most of the identified strains were resistant to clindamycin, erythromycin, and oxytetracycline. The isolated strains harbored blaTEM, qnrA and tetA. Approximately 96.2% of E. coli and 62.5% of Salmonella isolates were MDR to different antimicrobial classes. Moreover, 10.5% of C. perfringens isolates were extensive drug-resistant (XDR) to seven antimicrobial classes, while 84.2% of them were MDR to various antimicrobial classes. The findings of this study provide a better understanding regarding the epidemiological aspects of bacterial illnesses and for the development of prevention techniques for this problem. The antibiograms recorded in this study highlight the dangers of indiscriminate antibiotic usage in diarrheic calves.

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Cloning and characterization of the iron uptake gene iutA from avian Escherichia coli

Cited by 8 publications

References 27 publications

Nanoparticles as Potential Novel Therapies for Urinary Tract Infections

Nanoparticles as Potential Novel Therapies for Urinary Tract Infections

Emerging Antibiotic Resistance and Plasmid DNA in Escherichia coli Isolated from Respiratory Tract Infections in Bangladesh

Risk Factors, Antibiotic Profile, and Molecular Detection of Virulence and Antibiotic Resistance Genes of Enteric Bacteria in Diarrheic Calves in Egypt

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