Enhanced production of manganese peroxidase by Phanerochaete chrysosporium

Abstract

Production of manganese-dependent peroxidase (MnP) by the white-rot fungus

Purification, characterization and application of novel alkaline protease from new Bacillus cereus UV-15 mutant

“…At 1% concentration of Tween-20 and Tween-80, the enzyme retained 7 8% and 94% relative activity respectively. The results are in agreement with the values reported by Gupta (31) that alkaline protease enzyme was un-affected by Tween 80 and the mechanism of Tween 80 to enhance the extracellular enzyme production (32). Triton X -100, SDS and H2O2 both 0.5% and 1% concentrations s l i g h t l y decreased the protease activity.…”

A practical culture technique for enhanced production of manganese peroxidase by Anthracophyllum discolor Sp4

“…MnP can generate H 2 O 2 by the oxidation of oxalic and glyoxylic acids, thus providing an endogenous source for extracellular H 2 O 2 (Urzúa et al, 1998). The pH ranges obtained in this study were adequate for the growth of white-rot fungi since they generally grew at pH 4.0-6.0, with an optimum for enzyme production between 4.5 and 5.0 (Ürek and Pazarlioğlu, 2007).…”

“…The loss in the enzyme activity has been well-correlated to the degree of agitation, as described by Venkatadri and Irvine (1990) for ligninases. Therefore, static cell cultures are desirable for the MnP production due to the increase in the contact area between the cells and oxygen without shear stress (Ürek and Pazarlioğlu, 2007). With the addition of wheat grain, the proteases activity was higher than the controls (without wheat grain, under static conditions, or on a shaker at 100 rpm) and reached 0.20 U/L after 13 days.…”

Hyperproduction of Manganese Peroxidase through Chemical Mutagenesis of Trametes versicolor IBL-04 and Optimization of Process Parameters

“…EMS-90 had an activity of 3637 U mL -1 and EB-60 had an activity of 2688 U mL -1 when manganese sulfate was used as a mediator, whereas other used mediators had little or no effect on MnP activity. Urek and Pazarlioglu (2007) also reported enhanced MnP activity by adding manganese sulfate in fermentation medium. Several studies have shown that ligninolytic enzyme production could be enhanced several folds by the addition of mediators (Gnanamani et al 2006;Minussi et al 2007).…”