A comparison between enzyme immunoassay and HPLC for ochratoxin A detection in green, roasted and instant coffee

Fujii, Simone; Ono, Elisabete Yurie Sataque; Ribeiro, Ricardo Marcelo Reche; Assunção, Fernanda Garcia Algarte; Takabayashi, Cássia Reika; Oliveira, Tereza Cristina Rocha Moreira de; Itano, Eiko Nakagawa; Ueno, Yoshio; Kawamura, Osamu; Hirooka, Elisa Yoko

doi:10.1590/s1516-89132007000200020

Cited by 32 publications

(25 citation statements)

References 25 publications

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“…Chromatographic methods require multiple sample preparation steps prior to detection (Chung et al, 2007). Enzyme-linked immunosorbent assay (ELISA) is the most common immunoassay technique used in OTA analysis due to simplicity and capability for parallel analysis of multiple samples (FujiiI, et al, 2007;Monaci and Palmisano, 2004;Visconti and De Girolamo, 2005). Even though more recently the use of biosensors allows a major portability, in situ analysis of OTA with similar selectivity and sensitivity to ELISAs and much shorter times of analysis (Goryacheva, et al, 2007;Parker and Tothill, 2009;Pohanka et al, 2007;Prieto-Simon et al, 2007).…”

Section: Figure 2 General Schematic Representation Of Biosensorsmentioning

confidence: 99%

Application of nanotechnology in detection of mycotoxins and in agricultural sector

Sertova¹

2015

JCEA

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A brief review of nanotechnology application in detection of mycotoxins and in agriculture sector was presented. Mycotoxins are secondary metabolites produced by fungi. Their toxicity is the reason for implementation of various screening methods to detect them. During the last years, the highlight was put on nanoscale materials included in biosensors, which were some of the smart devices used for determination of mycotoxins, and in agriculture sector. Over the next decade, the progress of nanotechnology will demonstrated a way to improve detection of contaminated feed and food. To achieve this purpose the innovations of nanomaterials reported every year would be applied. In the paper, some of the applications developed by nanotechnology that would contribute to the implementation of new tools for analysis of mycotoxins and agricultural products were discussed.Keywords: agriculture, biosensors, mycotoxins, nanoparticles, nanotechnology Резюме Представен е кратък преглед за приложение на нанотехнологиите за откриване на микотоксини, както и в аграрния сектор. Микотоксините са вторични метаболити, произвеждани от гъбички. Тяхната токсичност е причина за прилагане на различни методи за откриването им. През последните години акцента е бил поставен върху нано материали, вградени в биосензори, които са част от модерните устройства, използвани за откриване на микотоксини, а също и в аграрния сектор. През следващото десетилетие ще бъде доказван напредъка на нанотехнологиите да бъдат използвани като метод за детекция на замърсени фуражи и храни. За постигането на тази цел всяка година ще се следят новостите при наноматериалите. В статията са обсъдени някои от приложенията,разработени с помощта на нанотехнологиите, които биха допринесли за изграждането на нови устройства за анализ на микотоксини и селскостопански продукти.

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Section: Figure 2 General Schematic Representation Of Biosensorsmentioning

confidence: 99%

Application of nanotechnology in detection of mycotoxins and in agricultural sector

Sertova¹

2015

JCEA

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“…ELISA is a reliable and rapid method for OTA determination in various kinds of commodities (Fujii et al, 2007;Matrella et al, 2006;Zheng et al, 2005). ELISA was preferred because it requires less sample volume and sample preparation is faster than other conventional methods such as TLC and HPLC (Zheng et al, 2005).…”

Section: Ochratoxin a Producing Abilitymentioning

confidence: 99%

Penicillium Species Isolated From Cocoa, Coffee Beans, and Dried Cassava in Yogyakarta Indonesia and Their Ochratoxin Production

Moulia

Setyabudi

Salleh

et al. 2014

IFNP

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The presence of Penicillium in cocoa and coffee beans, and dried cassava are detrimental due to its ability in ochratoxin A (OTA) production which carcinogenic and mutagenic to human. Objectives of this study were to isolate and identify Penicillium from cocoa, coffee beans and dried cassava in Yogyakarta by morphological and molecular characteristics, as well as to observe the ability of these isolates in OTA production on Yeast Extract Sucrose Agar (YES) medium. In this study, morphological characteristics were mainly based on the growth of isolates on identification media, while molecular characteristics were based on the similarity of PCR products using ITS4 and ITS5 as primers. OTA was detected by ELISA and UPLC methods. The result showed that 15 of 16 representative isolates obtained during this study were identified as Penicillium citrinum, one of the representative isolate from cocoa beans was identified as Penicillium paneum. Surprisingly, 13 among 15 of the obtained P. citrinum isolates from cocoa and coffee beans were positive in the production of OTA in YES medium, at the concentration of 4.64 to 25.26 µg/g media, while OTA was not detected in YES grown media by P. paneum and two isolates of P. citrinum from dried cassava. Conclusion of this study, the most found species Penicillium in cocoa and coffee beans were belong to P. citrinum which likely have a capability in the production of OTA.

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“…The extract is usually purified using solid-phase extraction, immunoaffinity column, or a combination of both. In order to analyze OTA in coffee, it is important to have an adequate clean up to remove substances like lipids and pigments that could interfere in the analytical techniques (FUJII et al, 2007;VARGAS et al, 2005;VENTURA et al, 2003).…”

Section: Extraction Proceduresmentioning

confidence: 99%

“…This mycotoxin has been found in food commodities such as cereals, oleaginous seeds, wine, meat, cocoa, spices, dried fruits, grapes, beer, green, roasted and instant coffee (ALMEIDA et al, 2007;BECKER et al, 1998;BRESH et al, 2000;BUCHELLI;TANIWAKI, 2002;BULLERMAN, 2003;FUJII et al, 2002FUJII et al, , 2007 potassium chloride (Merck,Germany,99.5%), potassium dichromate (Merck, Germany, 99.5%), sulfuric acid (Merck, Germany, 97.0%), methanol HPLC grade (Tedia, USA, 99.9%), trifluoroacetic acid HPLC grade (Tedia, USA, 99.8%), acetone pesticide grade (Tedia, USA, 99.8%), glacial acetic acid (Tedia, USA, 99.9%), toluene HPLC grade (Tedia, USA, 99.8%), benzene (Merck, Germany, 99.5%), acetone A.C.S. grade (Tedia, USA, 99.8%), ethyl alcohol (Quimes, Brazil, 95.0%), sodium hypochlorite (Invema, Brazil, 12.0%) and alcaline extran (Merck, Germany).…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a method for detection and quantification of ochratoxin A in green coffee using liquid chromatography coupled to mass spectrometry

et al. 2012

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A method using Liquid Chromatography Tanden Mass Spectrometry (LC-MS/MS) with matrix-matched calibration curve was developed and validated for determining ochratoxin A (OTA) in green coffee. Linearity was found between 3.0 and 23.0 ng.g-1. Mean recoveries ranged between 90.45% and 108.81%; the relative standard deviation under repeatability and intermediate precision conditions ranged from 5.39% to 9.94% and from 2.20% to 14.34%, respectively. The limits of detection and quantification were 1.2 ng.g-1 and 3.0 ng.g-¹, respectively. The method developed was suitable and contributed to the field of mycotoxin analysis, and it will be used for future production of the Certified Reference Material (CRM) for OTA in coffee.

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A comparison between enzyme immunoassay and HPLC for ochratoxin A detection in green, roasted and instant coffee

Abstract: An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ochratoxin A (OTA)

Cited by 32 publications

References 25 publications

Application of nanotechnology in detection of mycotoxins and in agricultural sector

Application of nanotechnology in detection of mycotoxins and in agricultural sector

Penicillium Species Isolated From Cocoa, Coffee Beans, and Dried Cassava in Yogyakarta Indonesia and Their Ochratoxin Production

Development and validation of a method for detection and quantification of ochratoxin A in green coffee using liquid chromatography coupled to mass spectrometry

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