Somatic embryogenesis from immature cotyledons of soybean (Glycine max (L.) Merr.): ontogeny of somatic embryos

Santos, Karla G. B. dos; Mariath, Jorge Ernesto de Araújo; Moço, Maria Cecília Chiara; Bodanese‐Zanettini, Maria Helena

doi:10.1590/s1516-89132006000100006

Cited by 15 publications

(10 citation statements)

References 23 publications

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“…2e, f, g, h). Closelyresembling patterns of development were also found in other species, including soybean (Santos et al 2006) and cowpea (Ramakrishnan et al 2005). Development of somatic embryos was asynchronous and continued during embryonic callus cultivation.…”

Section: Resultsmentioning

confidence: 68%

Optimization of horse chestnut (Aesculus hippocastanum L.) somatic embryo conversion

Troch

Werbrouck

Geelen

et al. 2009

Plant Cell Tiss Organ Cult

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Factors affecting conversion of horse chestnut (A. hippocastanum L.) somatic embryos into plantlets were evaluated. Anther filament derived embryogenic tissue developed bipolar structures with two cotyledons and a well-developed shoot and root apical meristem upon auxin omittance from the culturing medium. The impact of carbohydrate type (glucose, fructose, sucrose and maltose) and concentration (3 and 6%) on somatic embryo maturation and conversion were evaluated. Although conversion frequencies were high for all treatments, overall quality of regenerated plantlets was poor. Increasing the carbohydrate concentration in the maturation medium did not increase conversion of somatic embryos or quality of regenerated plantlets in terms of shoot height. On the contrary, addition of PEG (polyethylene glycol) in maturation media had a beneficial effect on shoot quality of regenerated plantlets. Sucrose was a superior carbon source when PEG was included in the maturation medium, in terms of conversion rate (65.7%) as well as of shoot quality of plantlets (43.8% of plantlets had shoots [2 cm). Clonal fidelity of the different development stages of somatic embryogenesis and of converted plantlets was assessed by flow cytometry and no major ploidy changes were found.

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Section: Resultsmentioning

confidence: 68%

Optimization of horse chestnut (Aesculus hippocastanum L.) somatic embryo conversion

Troch

Werbrouck

Geelen

et al. 2009

Plant Cell Tiss Organ Cult

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“…Several studies have shown a similarity in the somatic and zygotic embryos in terms of their morphological, histological, biochemical, and physiological aspects, and have introduced SE as a model for studying ZE ( Zimmerman, 1993 ; Palada-Nicolau and Hausman, 2001 ; review in Winkelmann, 2016 ). However, there is a very limited number of publications that refer to the development of somatic embryos that is caused by the specificity of this material as well as limitations in the available analytical methods ( Santos et al, 2006 ; Kumar et al, 2007 ; Kurczyńska et al, 2007 ; Nowak et al, 2012 ; Baskaran and Van Staden, 2017 ) and none of them has shown the molecular aspect of somatic embryo development. Performing WISH in explants that were undergoing SE in Arabidopsis enabled specific miR390 signals to be detected in the somatic embryos during different stages of development ( Figure 6 ).…”

Section: Resultsmentioning

confidence: 99%

Whole Mount in situ Localization of miRNAs and mRNAs During Somatic Embryogenesis in Arabidopsis

et al. 2018

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Somatic embryogenesis (SE) results from the transition of differentiated plant somatic cells into embryogenic cells that requires the extensive reprogramming of the somatic cell transcriptome. Commonly, the SE-involved genes are identified by analyzing the heterogeneous population of explant cells and thus, it is necessary to validate the expression of the candidate genes in the cells that are competent for embryogenic transition. Here, we optimized and implemented the whole mount in situ hybridization (WISH) method (Bleckmann and Dresselhaus, 2016; Dastidar et al., 2016) in order to analyze the spatiotemporal localization of miRNAs (miR156, miR166, miR390, miR167) and mRNAs such as WOX5 and PHABULOSA-target of miR165/166 during the SE that is induced in Arabidopsis explants. This study presents a detailed step-by-step description of the WISH procedure in which DIG-labeled LNA and RNA probes were used to detect miRNAs and mRNAs, respectively. The usefulness of the WISH in the functional analysis of the SE-involved regulatory pathways is demonstrated and the advantages of this method are highlighted: (i) the ability to analyze intact non-sectioned plant tissue; (ii) the specificity of transcript detection; (iii) the detection of miRNA; and (iv) a semi-quantitative assessment of the RNA abundance.

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“…Even in the biotechnology of plant cells, we are faced with fundamental difficulties. For example, the possibility of somatic embryogenesis in such important cultivated plants as soybeans (for a review, see Dos Santos et al, 2006) is severely limited. However, many obstacles impede the application of somatic embryogenesis in the biotechnology of economically important animals.…”

Section: Transformation Instead Of Transplantationmentioning

confidence: 99%

Structure-Function Analysis of Transformation Events

Zhuravlev¹,

Аветисов²

2011

Genetic Transformation

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Somatic embryogenesis from immature cotyledons of soybean (Glycine max (L.) Merr.): ontogeny of somatic embryos

Cited by 15 publications

References 23 publications

Optimization of horse chestnut (Aesculus hippocastanum L.) somatic embryo conversion

Optimization of horse chestnut (Aesculus hippocastanum L.) somatic embryo conversion

Whole Mount in situ Localization of miRNAs and mRNAs During Somatic Embryogenesis in Arabidopsis

Structure-Function Analysis of Transformation Events

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