Protective effect of sodium selenite on genotoxicity to human whole blood cultures induced by aflatoxin B1

Geyikoğlu, Fatime; Türkez, Hasan

doi:10.1590/s1516-89132005000800006

Cited by 24 publications

(21 citation statements)

References 24 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The enzyme inactivations induced by AFB 1 also corroborate by the genotoxic findings. The results obtained by us indicate a significant increase in the ratios of the SCEs and MNs in lymphocytes, which is in accordance with previous reports (Groopman and Kensler 1999;Geyikoglu and Turkez 2005). The SCEs are formed by toxic oxygen metabolites in cultured human leukocytes and other mammalian cells (Weitberg et al 1983).…”

Section: Discussionsupporting

confidence: 93%

“…Experiments were conform to the guidelines of the World Medical Assembly (Declaration of Helsinki). The concentrations were selected according to the previous studies (Yamashoji and Isshiki 2001;Geyikoglu and Turkez 2005;Turkez et al 2007). After supplementation of BA and AFB 1 , the blood was incubated for 1 h at 37°C to adjust body conditions, except for testing SCE and MN (see below).…”

Section: Experimental Designmentioning

confidence: 99%

See 1 more Smart Citation

Boric acid: a potential chemoprotective agent against aflatoxin b1 toxicity in human blood

Türkez

Geyikoğlu

2010

Cytotechnology

Self Cite

View full text Add to dashboard Cite

Aflatoxin B 1 is the most potent pulmonary and hepatic carcinogen. Since the eradication of Aflatoxin B 1 contamination in agricultural products has been difficult, the use of natural or synthetic free radical scavengers could be a potential chemopreventive strategy. Boric acid is the major component of industry and its antioxidant role has recently been reported. The present study assessed, for the first time, the effectiveness of boric acid following exposure to Aflatoxin B 1 on human whole blood cultures. The biochemical characterizations of glutathione and some enzymes have been carried out in erythrocytes. Alterations in malondialdehyde level were determined as an index of oxidative stress. The sister-chromatid exchange and micronucleus tests were performed to assess DNA damages in lymphocytes. Aflatoxin B 1 treatment significantly reduced the activities of antioxidants by increasing malondialdehyde level (30.53 and 51.43%) of blood, whereas, the boric acid led to an increased resistance of DNA to oxidative damage induced by Aflatoxin B 1 in comparison with control values (P \ 0.05). In conclusion, the support of boric acid was especially useful in Aflatoxin-toxicated blood. Thus the risk on tissue targeting of Aflatoxin B 1 could be reduced ensuring early recovery from its toxicity.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Experimental Designmentioning

confidence: 99%

Boric acid: a potential chemoprotective agent against aflatoxin b1 toxicity in human blood

Türkez

Geyikoğlu

2010

Cytotechnology

Self Cite

View full text Add to dashboard Cite

show abstract

“…The results obtained by us indicate a significant increase in the ratios of the SCEs and MNs by AFB1 in lymphocytes, which is in accordance with the previous reports (Groopman and Kensler 1999;Geyikoglu and Turkez 2005;Turkez and Geyikoglu 2010). The SCEs are formed by toxic oxygen metabolites in cultured human leukocytes and other mammalian cells (Weitberg et al 1983).…”

Section: Discussionsupporting

confidence: 92%

“…It is known that AFB1 has pro-oxidant effects (Geyikoglu and Turkez 2005;Adam and Malgorzata 2008;Turkez and Sisman 2007;Turkez et al 2010b). Treatment with BX could cause reductions in the activity levels of some pro-oxidant enzymes consequently ameliorating the tissue damage (Pawa and Ali 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Experiments conformed to the guidelines of the World Medical Assembly (Declaration of Helsinki). These investigations are based on the previous works (Yamashoji and Isshiki 2001;Geyikoglu and Turkez 2005;). After supplementation of BX and AFB1, the blood was incubated for 72 h at 37°C to adjust body conditions for testing SCE and MN.…”

Section: Experimental Designmentioning

confidence: 99%

See 1 more Smart Citation

In vitro studies on chemoprotective effect of borax against aflatoxin B1-induced genetic damage in human lymphocytes

et al. 2012

View full text Add to dashboard Cite

A common dietary contaminant, aflatoxin B1 (AFB1), has been shown to be a potent mutagen and carcinogen in humans and many animal species. Since the eradication of AFB1 contamination in agricultural products has been rare, the use of natural or synthetic free radical scavengers could be a potential chemopreventive strategy. Boron compounds like borax (BX) and boric acid are the major components of industry and their antioxidant role has recently been reported. In the present report, we evaluated the capability of BX to inhibit the rate of micronucleus (MN) and sister chromatid exchange (SCE) formations induced by AFB1. There were significant increases (P \ 0.05) in both SCE and MN frequencies of cultures treated with AFB1 (3.12 ppm) as compared to controls. However, co-application of BX (1, 2 and 5 ppm) and AFB1 resulted in decreases of SCE and MN rates as compared to the group treated with AFB1 alone. Borax gave 30-50 % protection against AFB1 induced SCEs and MNs. In conclusion, the support of borax was especially useful in aflatoxintoxicated blood tissue. Thus, the risk on target tissues of AFB1 could be reduced and ensured early recovery from its toxicity.

show abstract

Protective effects of cyclosativene on H2O2-induced injury in cultured rat primary cerebral cortex cells

et al. 2014

View full text Add to dashboard Cite

Sesquiterpenes have attracted much interest with respect to their protective effect against oxidative damage that may be the cause of many diseases including several neurodegenerative disorders and cancer. Our previous unpublished work suggested that cyclosativene (CSV), a tetracyclic sesquiterpene, has antioxidant and anticarcinogenic features. However, little is known about the effects of CSV on oxidative stress induced neurotoxicity. We used hydrogen peroxide (H 2 O 2 ) exposure for 6 h to model oxidative stress. Therefore, this experimental design allowed us to explore the neuroprotective potential of CSV in H 2 O 2 -induced toxicity in newborn rat cerebral cortex cell cultures for the first time. For this aim, MTT and lactate dehydrogenase release assays were carried out to evaluate cytotoxicity. Total antioxidant capacity (TAC) and total oxidative stress (TOS) parameters were used to evaluate oxidative changes. In addition to determining of 8-hydroxy-2-deoxyguanosine (8-OH-dG) levels, the single cell gel electrophoresis (or Comet assay) was also performed for measuring the resistance of neuronal DNA to H 2 O 2 -induced challenge. Our results showed that survival and TAC levels of the cells decreased, while TOS, 8-OH-dG levels and the mean values of the total scores of cells showing DNA damage (Comet assay) increased in the H 2 O 2 alone treated cultures. But pretreatment of CSV suppressed the cytotoxicity, genotoxicity and oxidative stress which were increased by H 2 O 2 . On the basis of these observations, it is suggested that CSV as a natural product with an antioxidant capacity in mitigating oxidative injuries in the field of neurodegenerative disorders.

show abstract

Protective effect of sodium selenite on genotoxicity to human whole blood cultures induced by aflatoxin B1

Abstract: The aim of this study was to investigate the effects of selenium and aflatoxin on human whole blood cultures (WBC) in relation to induction of sister-chromatid exchange (SCE

Cited by 24 publications

References 24 publications

Boric acid: a potential chemoprotective agent against aflatoxin b1 toxicity in human blood

Boric acid: a potential chemoprotective agent against aflatoxin b1 toxicity in human blood

In vitro studies on chemoprotective effect of borax against aflatoxin B1-induced genetic damage in human lymphocytes

Protective effects of cyclosativene on H2O2-induced injury in cultured rat primary cerebral cortex cells

Contact Info

Product

Resources

About