Biodistribution of free 99mTc-ovalbumin and 99mTc-ovalbumin encapsulated in liposomes

Abstract

ABSTRACT

Digestion, Absorption and Tissue Distribution of Ovalbumin and Palmitoyl‐ovalbumin: Impact on Immune Responses Triggered by Orally Administered Antigens

“…Ovalbumin (Grade V, Sigma, USA) and palmitoyl‐Ova antigens were incubated for 20 min at room temperature with 10 μ l of SnCl 2 (2 mg/ml SnCl 2 in 0.25 m HCl), 10 μ l of NaBH 4 (2 mg/ml NaBH 4 in 0.1 m NaOH) and 4 MBq of 99m Tc. The labelling yield, assessed by the method described by our group [38] was 95 ± 0.5% ( n = 50). The mixture was concentrated with a disposable microconcentrator (Anincon, M r cut‐off > 30.000).…”

Expression of costimulatory molecules in antigen-activated peritoneal macrophages treated with either ovalbumin or palmitoyl-ova conjugates

“…The underlying mechanisms by which lipopeptides, mucosal adjuvants and antigen delivery systems elicit both local and systemic immune responses without an adjuvant are only partially understood. The mechanism of action is assumed to be a complex multistep phenomenon resulting from the physiological conditions and direct interactions of antigens with different cell types at the presumed site of uptake and immune induction (Alves et al 2005;Oliveira et al 2007). An elegant study, performed by two-photon excitation, characterized the antigen-specific CD4+ T cells in vivo in real time during the induction of oral priming versus oral tolerance (Zinselmeyer et al 2005).…”