Evaluation of the phagocitary capacity of the mononuclear system in an experimental model of obstructive jaundice employing Tc- 99m Escherichia coli

Abstract

Infections and endotoxemia continue to be the principal causes of morbidity and mortality of patients with obstructions of the bile duct. The objective of the present work was the investigation of the phagocitary capacity of the mononuclear system in an experimental model of obstructive jaundice utilizing Tc-99m E.coli. The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) were significantly higher in jaundiced rats than in the control animals (p < 0.001). The liver of the jaundiced animals presented a significant reduction in Tc-99m E.coli uptake when compared to the controls (p < 0.001). The data also showed that there was a significant increase in the uptake of Tc-99m E.coli by the lungs of jaundiced rats (p < 0.01). The histological analyses of the liver of jaundiced rats showed an intense and diffuse proliferation of the bile ducts and an intensified polyploidy of the hepatocytes (mean volume = 843 µm³), but no significant alterations were observed in the lungs of either group. This dates could contribute to a better understanding of the mechanisms involved in cases of bacteremia, renal failure and pulmonary dysfunction observed in clinical analyses of obstructive jaundice.<br>Nos pacientes com obstrução do ducto biliar, as infecções e a endotoxemia continuam sendo uma das principais causas de morbidade e mortalidade. O objetivo desse trabalho foi investigar a capacidade fagocitária do sistema mononuclear, em um modelo experimental de icterícia obstrutiva, utilizando Tc-99m E.coli. Os níveis de aspartato aminotransferase (AST), alanina aminotransferase (ALT) e fosfatase alcalina (PAL) nos ratos com ligadura do ducto biliar comum (CBD) encontram-se significativamente mais elevados do que nos ratos sham. (p < 0.001). O fígado dos animais ictéricos apresentou uma significativa redução na captação da Tc-99m E.coli quando comparado com o controle. Os dados mostraram também, que houve um aumento significativo na captação da Tc-99m E.coli pelo pulmão dos ratos ictéricos (p < 0.01). O exame histológico dos cortes de fígado dos animais ictéricos apresentou proliferação intensa e difusa dos ductos biliares e uma acentuada poliploidia dos hepatócitos (volume médio: 843 µm³), Não foram observadas alterações significativas nos pulmões de nenhum grupo

Effects of the povidone-iodine (PVPI) in treatment of bacterial peritonitis induced in rats

“…The bacterial suspension infused into peritoneal cavity was obtained from a sample of standard Escherichia coli that, after growth in plate, was marked with 99mTecnetium (99mTc) as technique described by Diniz 7 . Concisely, aliquots of 2 ml of the bacterial suspension were incubated to 37ºC with 580 µM of stannous chloride with pH 7.0 for 10 minutes.…”