Effect on the enzymatic hydrolysis of lipids from dairy wastewater by replacing Gum Arabic emulsifier for sodium chloride

Mendes, Adriano A.; Castro, Heizir F. de

doi:10.1590/s1516-89132005000400017

Cited by 27 publications

(16 citation statements)

References 13 publications

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“…Published results suggest that the use of several salts have been shown positive effects in the lipase activity and consequently, increase the formation of free fatty acids (Bauduin et al 2004;Mendes and Castro 2005). For PPL the addition of several salts at a fixed concentration of 20 mM in the substrate medium resulted in either activation or inhibition of the hydrolytic activity as displayed in Fig.…”

Section: Resultsmentioning

confidence: 84%

“…Another additional effect of the NaCl in the medium can be related to the anion Clthat has also been described as beneficial to lipase catalyzed reactions, as a thermal stabilizer (Noel and Combes 2003). On the other hand, the effect of NaCl addition was found to be also remarkably depended with respect to the enzyme source (Mendes and Castro 2005).…”

Section: Resultsmentioning

confidence: 97%

“…The positive effect of increasing NaCl concentration in the substrate may be explained by the fact that, during the hydrolysis, the pH at the lipid/lipase interface can become lower than in the bulk solution due to the release of long chain fatty acids (Brockerhoff and Jensen 1974;Mendes and Castro 2005). Therefore, NaCl addition would help to increase the pH at the interface where the enzyme/substrate complex is formed, with the Na + replacing the H + ions around the enzyme.…”

Section: Resultsmentioning

confidence: 99%

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Enzymatic hydrolysis of soybean oil using lipase from different sources to yield concentrated of polyunsaturated fatty acids

Freitas

Bueno

Pérez

et al. 2007

World J Microbiol Biotechnol

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The ability of three commercially available lipases to mediate the hydrolysis of the soybean oil to yield concentrated of essential fatty acids was evaluated. The tested lipases were from microbial (Candida rugosa and Thermomyces lanuginosa) and animal cells (Porcine pancreatic lipase). In terms of free fatty acids, microbial lipases were more effective to promote the enzymatic hydrolysis of the soybean oil (over 70%) than the porcine pancreatic lipase (24%). In spite of this, porcine pancreatic lipase (PPL) showed the most satisfactory specificity towards both essential fatty acids and was, therefore, chosen to carry out additional studies. An experimental design was performed taking into consideration the enzyme and NaCl amounts as independent variables. The main effects were fitted by multiple regression analysis to a linear model and maximum fatty acids concentration could be obtained using 3.0 wt% of lipase and 0.08 wt% of NaCl. The mathematical model representing the hydrolysis degree was found to describe adequately the experimental results. Under these conditions, concentrations of 29.5 g/L and 4.6 g/L for linoleic and linolenic acids, respectively, were obtained.

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Section: Resultsmentioning

confidence: 84%

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Enzymatic hydrolysis of soybean oil using lipase from different sources to yield concentrated of polyunsaturated fatty acids

Freitas

Bueno

Pérez

et al. 2007

World J Microbiol Biotechnol

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“…Os lipídios são componentes importantes nos efluentes, pois causam poluição ambiental severa podendo formar filmes na superfície da água prevenindo a difusão de oxigênio e consequentemente causando a morte de muitas formas de vida aquática (MENDES; CASTRO, 2005). Estratégias para a remoção deste tipo de resíduo têm sido propostas como a utilização de enzimas e de biossurfactantes.…”

Section: Introductionunclassified

Produção de Lipase e Biossurfactante por Isolado de Efluente de Laticínio

Gasparin

Magri

Neves

et al. 2012

Biochem. Biotechnol. Rep.

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Lipases and biosurfactants are useful tools in the decontamination of polluted environments with lipid residues can be obtained from microbiological sources. The objective of this study was to evaluate the production of lipase and biosurfactant of isolated from wastewater of a dairy called ISO 6. Different vegetable oil 1% were tested as carbon source for production of lipase and biosurfactant. The lipolytic activity was quantified by measuring p-nitrophenol released after 10 minutes of incubation of the enzyme with p-nitrophenyl palmitate at 37 ° C and pH 8, 5. To determine emulsification index 2 mL of crude extract was mixed with 2 mL of kerosene and after shaking on vortex for 2 minutes was calculated the ratio between the height of the emulsified layer divided by the total height of the liquid column. The best carbon source for the production of lipase was corn oil (75.81 IU / mL) but it was not a good substrate for production of the biosurfactant, for the lowest rate of emulsification was obtained with this vegetable oil. In all experiments the isolated ISO 6 was able to produce biosurfactant and lipase showing potential for use in bioremediation of polluted environments with lipid residues. Key RESUMOAs lipases e biossurfactantes são ferramentas úteis na descontaminação de ambientes poluídos com resíduos lipídicos podendo ser obtidos de fontes microbiológicas. O objetivo deste estudo foi avaliar a produção de lipase e de biossurfactante de um isolado de efluente de laticínio denominado de ISO 6. Diferentes óleos vegetais a 1% foram testados como fonte de carbono para produção de lipase e biossurfactante. A atividade lipolítica foi quantificada pela dosagem de p-nitrofenol liberado após 10 minutos de incubação da enzima com palmitato de p-nitrofenila a 37o C e pH8,5. Para determinar o índice de emulsificação foi misturado 2 mL do extrato bruto com 2 mL de querosene e após agitação em vórtex por 2 minutos foi calculado a razão entre a altura da camada de emulsificação pela altura total do volume. A melhor fonte de carbono para a produção de lipase foi o óleo de milho (75,81UI/mL), mas este substrato o não foi bom para a produção de biossurfactante, pois o menor índice de emulsificação foi obtido com este óleo vegetal. Em todos os experimentos o isolado ISO 6 foi capaz de produzir lipase e biossurfactante demonstrando potencial para ser utilizado em biorremediação de ambientes poluídos com resíduos lipídicos.Palavras-chave: lipase, biossurfactante, biorremediação

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“…Activities of some exoenzymes, such as proteases, lipases, and chitinases have been reported in literature [14,15]. Recently, various research reports have described the use of microorganisms and/or enzyme pools developed for enhancing the treatability of various waste streams, such as dairy wastewater [16][17][18], landfill leachate [9], tannery wastewater [10], anaerobically digested biosolids [19], distillery wastewater [20], olive mill wastewater [21], foundry wastewater [22], and phenolic wastewater [23]. The use of extracellular enzymes in anaerobic digestion to increase the yield and rate of hydrolysis of complex polymeric components has also been demonstrated.…”

Section: Introductionmentioning

confidence: 99%

Effect of extracellular enzyme activity on digestion performance of mesophilic UASB reactor treating high-strength municipal wastewater

Turkdogan-Aydınol

Yetilmezsoy

Comez

2010

Bioprocess Biosyst Eng

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Effect of extracellular enzyme activity on digestion performance of up-flow anaerobic sludge blanket (UASB) reactor was investigated for enhancement of anaerobic treatability of municipal wastewater. Two identical UASB reactors (9 L), namely Reactor-A (without enzyme addition) and Reactor-B (with enzyme addition),were simultaneously operated at mesophilic conditions (32 ± 2°C) with a hydraulic retention time of 24 h. Preliminary test results showed that the highest total chemical oxygen demand (TCOD) removal were achieved with an extracellular enzyme dosage of 0.2 mL/L. In the activation period of the extracellular enzyme (on days 186-212), while Reactor-A removed up to 69.3% of TCOD and 55.9% of soluble chemical oxygen demand (SCOD), Reactor-B effectively removed up to 81.9% of TCOD and 72.2% of SCOD. The average VFA/alkalinity ratios were determined to be about 0.40 (±0.03) and 0.28 (±0.08) for Reactor-A and Reactor-B, respectively.

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Effect on the enzymatic hydrolysis of lipids from dairy wastewater by replacing Gum Arabic emulsifier for sodium chloride

Cited by 27 publications

References 13 publications

Enzymatic hydrolysis of soybean oil using lipase from different sources to yield concentrated of polyunsaturated fatty acids

Enzymatic hydrolysis of soybean oil using lipase from different sources to yield concentrated of polyunsaturated fatty acids

Produção de Lipase e Biossurfactante por Isolado de Efluente de Laticínio

Effect of extracellular enzyme activity on digestion performance of mesophilic UASB reactor treating high-strength municipal wastewater

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