ATP - bioluminescence as a technique to evaluate the microbiological quality of water in food industry

Costa, Patrícia Dolabela; Andrade, Nélio José de; Passos, Frederico José Vieira; Brandão, Sebastião César Cardoso; Rodrigues, Carolina Gonçalves Freire

doi:10.1590/s1516-89132004000300010

Cited by 18 publications

(10 citation statements)

References 8 publications

(6 reference statements)

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“…Some research [42] indicates that the ATP test can be used to optimize the work of water treatment plants. It is particularly useful for smaller water intakes in which water treatment mainly consists in removing iron, manganese and suspended solids.…”

Section: Microbiological Analyses Of Drinking Watermentioning

confidence: 99%

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Using ATP tests for assessment of hygiene risks

Pistelok

Pohl

Stuczyński

et al. 2016

Ecological Chemistry and Engineering S

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Adenosine-5'-triphosphate (ATP) is a high-energy molecule playing a role of universal energy carrier used in cell metabolism. Since ATP is present in both prokaryotic and eukaryotic cells, this coenzyme can be used to determine a hygiene state. During last decade there has been a significant growth of practical applications of ATP measurements. ATP tests are commonly used for an assessment of hygienic conditions in food industry, residential buildings and hotels. In comparison with traditional and time-consuming laborious microbial analysis the measurement of ATP provides a quick indicative on-site data, characterizing microbial and organic contamination to support decision-making with regards to adequate corrective and preventive actions. This paper demonstrates applications of ATP in practice and, particularly, in water treatment technology and wastewater processing. The review highlights opportunities for using the test for the optimization of water treatment facilities and pipe networks functioning in order to assess wastewater toxicity and wastewater treatment process efficiency. Other areas of application are also addressed. The review of literature indicates that ATP used in different areas of industry can be considered as a diagnostic method -complement the classical methods -useful for identification of hygiene risks as well as a tool for a real-time management of health and environmental risks.

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Section: Microbiological Analyses Of Drinking Watermentioning

confidence: 99%

“…As a result, it can bring savings as the number of filter bed flushings and the time these processes take are reduced. Moreover, extending the filtration cycle should improve water quality in the system [8,42].…”

Section: Microbiological Analyses Of Drinking Watermentioning

confidence: 99%

Using ATP tests for assessment of hygiene risks

Pistelok

Pohl

Stuczyński

et al. 2016

Ecological Chemistry and Engineering S

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“…Existen en la actualidad diversas formas para evaluar la calidad de la desinfección en superficies y la cantidad de presencia de materia orgánica, principal reservorio de microorganismos; para esto, en el momento se cuenta con fotoluminometría de adenosín trifosfato [ATP] en donde el ATP reacciona con el complejo enzimá-tico luciferin-luciderasa, produciendo un nivel de intensidad de luz, por lo que la concentración de ATP en la superficie es directamente proporcional a la intensidad de la luz producida, expresada en unidades relativas de luz (URL). "El nivel de bioluminiscencia de ATP corresponde, entonces, a la carga biológica presente en una superficie o elemento, con capacidad de permitir el crecimiento de microorganismos" (9).…”

Section: Introductionunclassified

Evaluación medioambiental de residuos hospitalarios peligrosos mediante luminometria y cultivos microbiológicos en una institución hospitalaria de Bogotá

Vargas

Cruz

2016

Rev. Colomb. Enferm.

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Introducción: el manejo adecuado de los residuos hospitalarios es un constante reto para la prevención en la propagación de infecciones y contaminación del medio ambiente. Objetivo: evaluar el manejo de residuos hospitalarios de riesgo infeccioso mediante luminometría de adenosín trifosfato (ATP) y cultivos microbiológicos en el Instituto del Corazón de Bucaramanga, sede Bogotá. Materiales y métodos: se realizó un estudio experimental de corte transversal prospectivo en el Instituto del Corazón de Bucaramanga, sede Bogotá en el periodo de agosto a diciembre de 2015; se aplicaron listas de verificación, medición de ATP y cultivos microbiológicos. Resultados: se encontraron diferencias estadísticamente significativas entre “Se cumple con la frecuencia y horarios establecidos para la recolección interna de residuos” (p valor= 0,001), “El tiempo de recolección de residuos dura entre 30–40 minutos” y “El personal encargado de la ruta sanitaria realiza higiene de manos al terminar el proceso” (p valor =0,005) respectivamente; para los otros ítems no se encontraron diferencias estadísticamente significativas (Valor p>0,005), solo una muestra cumplía el estándar (Cumplimiento ≤25 Unidades Relativas de luz (URL), y en el aislamiento microbiológico se evidenció el crecimiento de un Hongo Filamentoso. Conclusión: es necesario estandarizar el procedimiento de limpieza en superficies ambientales y monitorizar el proceso de ruta sanitaria de residuos peligrosos en la institución.

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“…Microbial biomass can be quantified by measuring ATP via bioluminescence, and because of this ATP bioluminescence technology has been widely applied in many fields in which microbial load/activity is important, including restaurant hygiene (Griffiths, 1996), medical environments (Griffith et al, 2000;Aycicek et al, 2006;Cooper et al, 2007), food and drink sanitation (Niza-Ribeiro et al, 2000;Lehto et al, 2011) drinking water quality (Delahaye et al, 2003;Costa et al, 2004), composting (Horiuchi et al, 2003;Tiquia et al, 2002), waste-activated sludge (Chu et al, 2008), soil research (Contin et al, 2000(Contin et al, , 2001 and ambient air monitoring (Lee and Chang, 2000;Seshdri et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

In Situ Rapid Evaluation of Indoor Bioaerosols Using an ATP Bioluminescence Assay

Lin

Wang

Hsien

et al. 2013

Aerosol Air Qual. Res.

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An adenosine-5'-triphosphate (ATP) bioluminescence method was developed for detecting microbial activity in indoor air. This method was compared with the traditional method of collecting, culturing and counting CFUs. A comparison of the results showed that ATP bioluminescence, expressed as RLUs, was moderately correlated with the entire set of CFU counts (r = 0.607), and that the correlation improved to r = 0.963 (p value < 0.001) when CFU outliers were removed from the calculations. The ATP bioluminescence method was applied at four different sites; a hospital Chinese medicine diagnostic room, a library, a government office, and a railway station lobby. The results showed that microbial activity was far higher in the railway station lobby than at the other three sites, and this is believed to be due to the higher volume and density of people in this space. At all four sites, higher microbial activity was linked to indoor plants, garbage cans, shoe racks, and furnished waiting areas. PCA of the data showed that microbial activity in the Chinese medicine diagnostic room was closely related to room temperature and humidity, and hence lowering the latter can reduce the potential for microbial activity at this site. At all four sites, no correlation was found between microbial activity and airborne pollutants. The ATP bioluminescence method was applied for the rapid evaluation of room disinfection using chloride dioxide, and results showed that twenty minutes after spraying with 100 ppm ClO 2 , microbial activity was reduced to 38.7% of its original level. ATP bioluminescence is simpler, easier to operate, and more cost-effective than the conventional microbial culture method of evaluating microbial load. The results obtained in this research confirm that the proposed ATP bioluminescence technique is capable of instantaneously detecting microbial activity in an indoor environment. Moreover, this approach can be used for on-line evaluation of room disinfection efficiency.

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ATP - bioluminescence as a technique to evaluate the microbiological quality of water in food industry

Cited by 18 publications

References 8 publications

Using ATP tests for assessment of hygiene risks

Using ATP tests for assessment of hygiene risks

Evaluación medioambiental de residuos hospitalarios peligrosos mediante luminometria y cultivos microbiológicos en una institución hospitalaria de Bogotá

In Situ Rapid Evaluation of Indoor Bioaerosols Using an ATP Bioluminescence Assay

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