Evaluation of possible failure of the mononuclear phagocyte system after total splenectomy in rats

Marques, Ruy Garcia; Petroianu, Andy; Oliveira, Mariana Borges; Bernardo-Filho, Mário; Portela, Margareth Crisóstomo

doi:10.1590/s1516-89132004000200006

Cited by 6 publications

(3 citation statements)

References 18 publications

(27 reference statements)

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“…As the largest lymphoid organ in the human body, the spleen plays a very important immune function, clearing bacteria from the bloodstream and providing the early production of antibodies in response to various antigenic particles. Its role in the immune system is highlighted by the number and different types of immune cells found in the organ temporarily or permanently, and by its peculiar anatomical and histological structure, which favours, among others, the functions of opsonization, phagocytosis and destruction of foreign antigens [1–6].…”

Section: Introductionmentioning

confidence: 99%

Critical mass of splenic autotransplant needed for the development of phagocytic activity in rats

Marques

Caetano²,

Diestel

et al. 2012

Clinical and Experimental Immunology

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SummaryWhen total splenectomy is inevitable, heterotopic splenic autotransplantation seems to be the only alternative to maintain the functions of the spleen. The present study was carried out to analyse the critical mass of splenic autotransplant (SAT) for the development of phagocytic activity in rats. Wistar rats were submitted to total splenectomy (TS) alone or in combination with slices of SAT ranging from an average rate of 21·9% (one slice) to 100% (five slices) of the total splenic mass implanted into the greater omentum. Sixteen weeks after the beginning of the experiment, the animals were inoculated intravenously with a suspension of Escherichia coli labelled with Tc-99m. After 20 min, the rats were killed and the liver, lung and spleen or SAT, as well as blood samples were removed to determine the percentage of labelled bacteria uptake in these tissues. As the percentage of the total splenic mass contained in the SAT increased, the bacteria remaining in the blood decreased. From the implant of 26% up to the implant of the total splenic mass (100%) there was no difference in the bacteria remaining in the blood between the healthy animals of the control group and those submitted to TS combined with SAT. This finding shows that the critical mass needed for the development of phagocytic activity of macrophages in splenic autotransplants in adult rats is 26% of the total splenic mass.

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Section: Introductionmentioning

confidence: 99%

Critical mass of splenic autotransplant needed for the development of phagocytic activity in rats

Marques

Caetano²,

Diestel

et al. 2012

Clinical and Experimental Immunology

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“…Our results reinforce the concept that, in intravenous infection by L. monocytogenes , the importance of the spleen is controversial, because splenectomized animals show greater control of bacterial multiplication and dissemination to other tissues (17–20, 23). On the other hand, in infection by other bacteria, such as S. pneumoniae, Neisseria meningitidis, Haemophilus influenzae, S. aureus , and Escherichia coli , the importance of the spleen in controlling these infections is well known (10, 11, 24–26).…”

Section: Discussionmentioning

confidence: 99%

Splenic autotransplantation reverses interferon‐gamma and nitric oxide production and resistance to Listeria monocytogenes in splenectomized mice

Perobelli

Alves

Rezende

et al. 2010

Transplant Infectious Dis

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Splenectomized mice control Listeria monocytogenes infection better than non-splenectomized mice. Here, BALB/c mice subjected to splenectomy and autogenous grafting of spleen were evaluated after 3 and 7 days of intravenous L. monocytogenes infection. The group of splenectomized animals (SP) presented a lower number of bacteria in the liver in comparison with both the sham-operated control group (CT) and the group that received splenic autotransplantation (AT) in the retroperitoneal site. The AT group presented bacterial counts in the liver similar to the CT group. SP animals showed larger production of interferon-gamma (IFN-γ) and nitric oxide (NO) in the liver in comparison with CT and AT, this being associated with greater accumulation of mononuclear cells. IFN-γ production by spleen cells after stimulation with heat-killed Listeria was similar between the AT and CT groups, suggesting that the implanted fragments behaved like the original organ. The autogenous grafting of spleen fragments reverses the resistance to L. monocytogenes infection found in splenectomized mice, associated with a reduced IFN-γ and NO production in the liver. The present study shows that splenic autotransplantation restores the function of the spleen in splenectomized mice, even though in this case it does favor the susceptibility to L. monocytogenes infection.

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“…99mTc has been the most utilized radionuclide in diagnosis nuclear medicine procedures to label compounds and cellular structures used as radiopharmaceuticals (Hladik III et al, 1987;Early & Sodee, 1995;Saha, 1998;Nigri et al, 2004). It has also been used to label biological structures (Plotkowski et al, 1993;Bernardo-Filho et al, 1992;Bernardo-Filho et al, 1993;Marques et al, 2004) in basic scientifi c research. This wide use of this radionuclide is due to its optimal physical characteristics, availability from 99Mo/99mTc generator and negligible environmental impact (Hladik III et al, 1987;Early;Sodee, 1995;Saha, 1998).…”

Section: Introductionmentioning

confidence: 99%

An aqueous extract of Pfaffia sp. does not alter the labeling of blood constituents with technetium-99m and the morphology of the red blood cells

Fernandes¹,

Brito²,

Frydman³

et al. 2005

Rev. bras. farmacogn.

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ABSTRACT:Plants have been used for the human beings as food, as additives and/or as medicines. There are controversies about the biological effects of several natural products and, it is worthwhile to try to develop experimental assays to evaluate properties of extracts of plants. Pfaffi a sp. is utilized in popular medicine and various properties have been attributed to its extract. Red blood cells (RBC) and plasma proteins are labeled with technetium-99m (Tc-99m) and this labeling procedure depends on a reducing agent and stannous ion is usually used. There are reports that drugs can alter the labeling of blood elements with Tc-99m. We have evaluated the infl uence of a Pfaffi a sp. extract on the labeling of blood constituents with Tc-99m and on the morphology of RBC. Blood was incubated with an aqueous extract of Pfaffi a sp., stannous chloride and Tc-99m. Samples were centrifuged and plasma and blood cells were separated and also precipitated with trichloroacetic acid. Soluble and insoluble fractions were separated. The results did not show alteration in the uptake of radioactivity and no modifi cations on the shape of the RBC in presence of Pfaffi a sp. Once this labeling process depends on a reducing agent, probably, this extract has compounds with anti-oxidant properties as already described elsewhere, that could protect the stannous ions against the oxidation process. This fact would aid the labeling process of blood elements with Tc-99m.

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Evaluation of possible failure of the mononuclear phagocyte system after total splenectomy in rats

Abstract: ABSTRACT

Cited by 6 publications

References 18 publications

Critical mass of splenic autotransplant needed for the development of phagocytic activity in rats

Critical mass of splenic autotransplant needed for the development of phagocytic activity in rats

Splenic autotransplantation reverses interferon‐gamma and nitric oxide production and resistance to Listeria monocytogenes in splenectomized mice

An aqueous extract of Pfaffia sp. does not alter the labeling of blood constituents with technetium-99m and the morphology of the red blood cells

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