Genetic variability in regenerated Metarhizium flavoviride protoplasts

Kuklinsky-Sobral, Júlia; Luna-Alves-Lima, Elza Áurea de; Araújo, Janete Magalí de; Azevedo, João Lúcio

doi:10.1590/s1516-89132004000100001

Cited by 4 publications

(2 citation statements)

References 12 publications

(13 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Introductionmentioning

confidence: 99%

“…There is a need for more coformulation studies to verify whether the pathogen will work synergistically or with wide host range and climatic conditions under which the fungus will infect its host. Such studies could also help to elucidate the conditions favoring recombination and the potential risks of displacement of indigenous strains by exotic entomogenous fungi (Kuklinsky et al, 2004). Genetic recombination would not only make it difficult to monitor exotic strains, but also the new genotypes could have pathogenicity traits quite distinct from those of their parents (Wang et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Tracking coformulated strains of the entomopathogenic fungi Beauveria bassianaand Metarhizium anisopliae using SCAR markers

Ummidi¹,

Vadlamani²

2016

Turk J Biol

View full text Add to dashboard Cite

This article examines the development of strain-specific sequence-characterized amplified region (SCAR) molecular markers in two strains of Beauveria bassiana and Metarhizium anisopliae, as well as their use for tracking pathogens in coinfected insect pests. The markers were designed based on the polymorphic introns of the large subunit region of the ribosomal DNA. These markers were used to distinguish between two strains of Beauveria (B55 and B51) and two strains of Metarhizium (M20 and M48). The entomopathogenic strains demonstrated synergistic increase in mortality against Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) larvae when infected with the coformulated M. anisopliae strain M20 + B. bassiana strain B55, particularly at a 2:1 proportion of LC 50 concentration. The study revealed a disparity between intergeneric and interstrain coformulations. In intergeneric coformulations, one strain appeared predominant over the other strain at 1:2 and 1:4 proportions, both under in vivo and in vitro conditions. On the other hand, in interstrain coformulations, both strains survived and formed heterokaryons. Molecular studies revealed that the heterokaryons were unstable and reverted back to any one of the parent strains after 3 or 4 generations.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Tracking coformulated strains of the entomopathogenic fungi Beauveria bassianaand Metarhizium anisopliae using SCAR markers

Ummidi¹,

Vadlamani²

2016

Turk J Biol

View full text Add to dashboard Cite

show abstract

Cloning and functional analysis of the second geranylgeranyl diphosphate synthase gene influencing helvolic acid biosynthesis in Metarhizium anisopliae

Singkaravanit

Kinoshita

Ihara

et al. 2010

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

A gene (ggs2) having high similarity to the geranylgeranyl diphosphate synthase (GGPP synthase) gene was cloned from Metarhizium anisopliae NAFF635007. The ggs2 gene (1,239-bp open reading frame with no intron) encoded a protein of 412 amino acids, and the transcription occurred only after late log-phase during the growth. Gene disruption of ggs2, performed to clarify the function in M. anisopliae, resulted in decreased GGPP synthase activity together with a slight delay of sporulation. An high performance liquid chromatography (HPLC) comparison of compound profiles between the wild-type strain and the disruptant revealed that a compound was abolished by the ggs2 disruption. Purification and structural elucidation by 1H-NMR and mass spectrometry analyses revealed that the lost compound is helvolic acid. Furthermore, the pathogenicity assay against two species of insect larvae revealed that the ggs2-disruptant possessed much weaker toxicity than the wild-type strain. Based on these results, it was concluded that ggs2 encodes the GGPP synthase influencing the biosynthesis of secondary metabolites in various species, including helvolic acid in M. anisopliae. To the best of our knowledge, this is the first report to identify a GGPP synthase gene related to secondary metabolism in entomopathogenic fungi.

show abstract

Formation of interspecies fusants of Agaricus bisporus and Agaricus bitorquis mushroom by protoplast fusion

2007

View full text Add to dashboard Cite

Interspecies fusants are formed between Agaricus bisporus and Agaricus bitorquis by protoplast fusion technique. Protoplasts were isolated and regenerated by using Novozyme 234 lytic enzyme. Twenty slow growing isolates were separated from the protoplast regenerated colonies, which were assumed as homokaryons (putative homokaryons). These twenty isolates were subjected to growth rate, colony morphology and spawn run studies for screening of true homokaryons. Antifungal markers were developed for selection of fusants.

show abstract

Genetic variability in regenerated Metarhizium flavoviride protoplasts

Abstract: Protoplast isolation and regeneration were evaluated in two wild

Cited by 4 publications

References 12 publications

Tracking coformulated strains of the entomopathogenic fungi Beauveria bassianaand Metarhizium anisopliae using SCAR markers

Tracking coformulated strains of the entomopathogenic fungi Beauveria bassianaand Metarhizium anisopliae using SCAR markers

Cloning and functional analysis of the second geranylgeranyl diphosphate synthase gene influencing helvolic acid biosynthesis in Metarhizium anisopliae

Formation of interspecies fusants of Agaricus bisporus and Agaricus bitorquis mushroom by protoplast fusion

Contact Info

Product

Resources

About