Isolation of alkalophilic CGTase-producing bacteria and characterization of cyclodextrin-glycosyltransferase

Abstract

One hundred and twenty five soil samples were collected from the regions of roots of corn, cassava, potato, bean, sugar cane, soya, and pumpkin. From these, 75

Characterisation of cyclodextrin glucanotransferase fromBacillus circulans ATCC 21783 in terms of cyclodextrin production

“…The addition of these ions in higher concentrations showed loss of CGTase activity in the presence of 2-8 mM CaCl 2 , while CoCl 2 in concentrations 1-4 mM maintained a 19-20% increase in cyclising activity (Table 3). Similar effect of 2-10 mM CaCl 2 is reported for CGTase of alkaliphilic Bacillus firmus (Higuti et al, 2003) unlike other strain Bacillus firmus maintaining 100% residual activity in the presence of 1-5 mM Ca 2+ (Yim et al, 1997). Generally, the effect of metal ions and reagents on the cyclising activity of the enzyme is depending on the bacterial producer (Fujita et al, 1990;Yim et al, 1997;Higuti et al, 2003).…”

“…Only CGTase from Bacillus agaradhaerens LS-3C is optimal active at pH 9.0 (Martins and Hatti-Kaul, 2002). Two pH optima (5.5 and 8.5) have been established for CGTase from alkaliphilic Bacillus firmus (Higuti et al, 2003) and Bacillus firmus NCIM5119 (Gawande et al, 1999).…”

Studies on alkalophilic CGTase-producing bacteria and effect of starch on cyclodextrin-glycosyltransferase activity

“…Isolate number 31 of strain of alkalophilic CGTase-producing bacteria, identified as Bacillus firmus by morphological, physiological and biochemical tests (Higuti et al, 2003), was used. It was grown for 6-7 days at 28 °C and 120 rpm in a New Brunswick rotary shaker.…”

Purification and Characterization of Eye-Specific Lactate Dehydrogenase C4Isozyme in Greenling (Hexagrammos otakii)

“…5). 이는 LDH C4 동위효소의 α-keto acid들에 대한 넓은 기질특이성에 기인하 는 효소촉매부위의 이온화정도 차이 때문이라고 생각된다 [15,21,38]. 따라서 LDH 활성측정 시 일반적으로 사용하는 중 성조건이 eye-specific LDH C4 동위효소의 활성측정 시에는 Fig.…”