Characterisation of cyclodextrin glucanotransferase fromBacillus circulans ATCC 21783 in terms of cyclodextrin production

Abstract

Cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19) from Bacillus circulans ATCC 21783 was purified by ultrafiltration and a consecutive starch adsorption. Total enzyme yield of 75.5% and purification factor of 13.7 were achieved. CGTase was most active at 65 ºC, possessed two clearly revealed pH-optima at 6.0 and 8.6 and retained from 75 to 100% of its initial activity in a wide range of pH, between 5.0 and 11.0. The cyclising activity was enhanced by 1 mM CaCl 2 or 4 mM CoCl 2 . The enzyme was thermostable up to 70 ºC, and 64% of the original activity remained at 70 ºC after 30 min heat treatment. Up to 41% conversion into cyclodextrins was obtained from 40 g l -1 starch without using any additives. This CGTase produced two types of cyclodextrins, beta and gamma, in a ratio 73:27 after 4 h reaction time at 65 ºC. This feature of the enzyme could be of interest for industrial cyclodextrin production.

A novel cyclodextrin glucanotransferase from an alkaliphile Microbacterium terrae KNR 9: purification and properties

“…Rosso et al ( 2005 ) described the CGTase purification from B. circulans using the starch adsorption method. Vassileva et al ( 2007 ) obtained purified CGTase with 87 % enzyme yield and 1.3-fold purification.…”

“…The optimal pH value obtained is in accordance with other reported pH values in the literature. The CGTases from B. circulans ATCC 21783 (Vassileva et al 2007 ), B. stearothermophilus ET1 (Chung et al 1998 ), Bacillus sp. G1 (Sian et al 2005 ), B. firmus No.…”

Alkaliphilic bacteria: applications in industrial biotechnology

“…The utility value attached to CGTases has also encouraged usage of CDs for improving diverse products such as food, cosmetics, and pharmaceuticals [25,41,242]. Some recent reports also document methods for enhanced production of CGTases, some from alkalitolerant organisms [17,32,43,44,93,107,117,148,160,168,174,175,215,219,260,261,282].…”

The maintenance of the list of QPS microorganisms intentionally added to food or feed - Scientific Opinion of the Panel on Biological Hazards

“…B. circulans has mainly been studied for applications in biotechnology for polysaccharide and protease production (Rao et al, 2008aand b, Kawamura and Matsuda, 2008, Bocchini et al, 2008, Vassilevai et al, 2007, Szerman et al, 2007, Funane et al, 2007. It has also been studied as a probiotic in aquaculture (Ghosh et al, 2003) and at least one strain of B. circulans is known to produce a nonhemolytic, lipopeptide biosurfactant with antimicrobial activities (Das et al, 2008).…”