Production and properties of Xylanase from thermophilic Bacillus sp.

Cordeiro, Carlos Alberto Martins; Martins, Meire Lélis Leal; Luciano, Angélica Bárbara; Silva, Roberta Freitas da

doi:10.1590/s1516-89132002000600002

Cited by 30 publications

(24 citation statements)

References 23 publications

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“…As case in point, paper and pulp industry is one of the distinguishing areas in which active and stable xylanase in high pH and temperature ranges is mighty pressing for their high quality of final products [10, 28, 29]. Accordingly, thermal and pH stabilities of xylanase, isolated from B. caldoxylolgticus , have been reported by Cordeiro et al [23]. Lama et al [3] also have reported a very high thermostable xylanase enzyme, isolated from Bacillus thermantarcticus .…”

Section: Discussionmentioning

confidence: 99%

Cost-Effective Production and Optimization of Alkaline Xylanase by Indigenous Bacillus mojavensis AG137 Fermented on Agricultural Waste

2011

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A xylanase producer Bacillus mojavensis strain, called AG137, isolated from cotton farm (Kashan-Iran). The optimal xylanase activity reached at 55°C & pH 9.0. Enzyme yield was studied using a medium with different agricultural wastes as inducers. Xylanase production of about 249.308 IU/mL was achieved at pH 8 and 37°C, within 48 h submerged fermentation in enzyme production medium supplemented with 2% (w/v) oat bran as an optimum carbon source. A mixture of 1% (w/v) yeast extract and 1% (w/v) tryptone as optimum nitrogen sources, agitation speed 200 rpm, and inoculum size 2% (v/v) were the optimums for maximum production. Accordingly, xylanase yield from 194.68 IU/mL under non-optimized fermentation condition enhanced to 302.466 IU/mL in optimized condition. Screened xylanase is thermostable, presenting 70% stability at 60°C during 30 min. Further enzyme incubation in higher temperature caused a decrease in the residual enzyme activity, yet it retained 68%–50% of its activity after 1 hour from 45°C to 55°C. Besides, it is stable in pH 9 and 10, maintaining over 70% of its activity for 2 h. The enzyme also could preserve 71% and 63% of its initial activity after 3 hours of pre-incubation in the same alkaline condition. Produced xylanase therefore was introduced as an alkaline-active and stable one, displaying suitable thermostability feature, confirmed by HPLC analysis. Hence, all xylanase properties highlight its promising uses in industrial scale.

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Section: Discussionmentioning

confidence: 99%

Cost-Effective Production and Optimization of Alkaline Xylanase by Indigenous Bacillus mojavensis AG137 Fermented on Agricultural Waste

2011

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“…No correlation between growth and xylanase production was observed. The increase in xylanase activity during the late exponential phase might be due to the autolysis of aged cells [11]. The xylanase produced by P. macerans IIPSP3 was named IIPSP3 xylanase.…”

Section: Production Of Enzymementioning

confidence: 99%

A novel thermostable xylanase of Paenibacillus macerans IIPSP3 isolated from the termite gut

Dheeran

Nandhagopal

Kumar

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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Xylanase is an enzyme in high demand for various industrial applications, such as those in the biofuel and pulp and paper fields. In this study, xylanase-producing microbes were isolated from the gut of the wood-feeding termite at 50°C. The isolated microbe produced thermostable xylanase that was active over a broad range of temperatures (40-90°C) and pH (3.5-9.5), with optimum activity (4,170 ± 23.5 U mg⁻¹) at 60°C and pH 4.5. The enzyme was purified using a strong cation exchanger and gel filtration chromatography, revealing that the protein has a molecular mass of 205 kDa and calculated pI of 5.38. The half-life of xylanase was 6 h at 60°C and 2 h at 90°C. The isolated thermostable xylanase differed from other xylanases reported to date in terms of size, structure, and mode of action. The novelty of this enzyme lies in its high specific activity and stability at broad ranges of temperature and pH. These properties suggest that this enzyme could be utilized in bioethanol production as well as in the paper and pulp industry.

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“…The Michaelis constants of the crude and purified xylanase preparations were 104 and 78times of that of commercial xylanase. Therefore the Bacillus pumilus xylanase has more potential than the commercial xylanse considered in this study and other studies (Melanocarpus albomyces xylanase has the K m of 0.3mgL Xylanases from Trichoderma launginosus [20] and Trichoderma launginosus [21] was inactivated very easily at acidic, neutral and alkaline pH values and at 70 o C. Xylanase from Bacillus sp was stable for 2 hours at the temperatures ranging from 30-50 o C [22]. Thermal stability of two different xylanases obtained from Melanocarpus albomyces was differed.…”

Section: Discussionmentioning

confidence: 70%

Comparison of the Kinetic Properties of Crude and Purified Xylanase from <i>Bacillus pumilus</i> with Commercial Xylanase from <i>Aspergillus niger</i>

Ranganathan

Arasaratnam

2012

Vingnanam J Sci

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Xylanases showing better stability at alkaline pH and higher temperatures have potential applications in several industrial processes. The objective of this study is to compare the kinetic properties and stability of crude and purified xylanase from Bacillus pumilus with commercial xylanase from Aspergillus niger. Bacillus pumilus which can grow and produce xylanase above 40 o C and pH 9.0 was selected for this study. Crude, purified and commercial xylanases showed zero order kinetics for 10 minutes and highest activity was obtained at 60 o C and pH 9.0. The optimum pH for crude, purified and commercial xylanases were 9.0, 9.0 and 7.0 respectively at 60 o C and the Michaelis constant by Lineweaver-Burk Plot for xylan were 3.1, 2.3 and 0.03gL -1 under the respective optimized conditions. The half-life of the crude and purified xylanase was highest at pH 9.0 and 50 o C while that for the commercial enzyme was at pH 7.0 and at 60 o C.

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Production and properties of Xylanase from thermophilic Bacillus sp.

Cited by 30 publications

References 23 publications

Cost-Effective Production and Optimization of Alkaline Xylanase by Indigenous Bacillus mojavensis AG137 Fermented on Agricultural Waste

Cost-Effective Production and Optimization of Alkaline Xylanase by Indigenous Bacillus mojavensis AG137 Fermented on Agricultural Waste

A novel thermostable xylanase of Paenibacillus macerans IIPSP3 isolated from the termite gut

Comparison of the Kinetic Properties of Crude and Purified Xylanase from <i>Bacillus pumilus</i> with Commercial Xylanase from <i>Aspergillus niger</i>

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