Comparison of the Kinetic Properties of Crude and Purified Xylanase from &lt;i&gt;Bacillus pumilus&lt;/i&gt; with Commercial Xylanase from &lt;i&gt;Aspergillus niger&lt;/i&gt;

Ranganathan, Kannan; Arasaratnam, Vasanthy

doi:10.4038/vingnanam.v10i1.4072

Cited by 6 publications

(2 citation statements)

References 11 publications

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“…Different ionizable groups with different p K a values are present on the surface of the protein molecules and surface charge distribution on the enzyme molecules varies with the pH on the environment. These fluctuations in charges may affect the enzyme activity either by changing the structure or by changing the charge of a residue important for substrate binding or catalysis [ 50 ]. The removal of proteases and protein fragments frequently present in the crude extract, which in some cases can be considered enzyme-stabilizing substances, can make the enzyme more susceptible to pH changes [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Agroindustrial Wastes as Alternative for Lipase Production by Candida viswanathii under Solid-State Cultivation: Purification, Biochemical Properties, and Its Potential for Poultry Fat Hydrolysis

et al. 2016

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The aims of this work were to establish improved conditions for lipase production by Candida viswanathii using agroindustrial wastes in solid-state cultivation and to purify and evaluate the application of this enzyme for poultry fat hydrolysis. Mixed wheat bran plus spent barley grain (1 : 1, w/w) supplemented with 25.0% (w/w) olive oil increased the lipase production to 322.4%, compared to the initial conditions. When olive oil was replaced by poultry fat, the highest lipase production found at 40% (w/w) was 31.43 U/gds. By selecting, yeast extract supplementation (3.5%, w/w), cultivation temperature (30°C), and substrate moisture (40%, w/v), lipase production reached 157.33 U/gds. Lipase was purified by hydrophobic interaction chromatography, presenting a molecular weight of 18.5 kDa as determined by SDS-PAGE. The crude and purified enzyme showed optimum activity at pH 5.0 and 50°C and at pH 5.5 and 45°C, respectively. The estimated half-life at 50°C was of 23.5 h for crude lipase and 6.7 h at 40°C for purified lipase. Lipase presented high activity and stability in many organic solvents. Poultry fat hydrolysis was maximum at pH 4.0, reaching initial hydrolysis rate of 33.17 mmol/L/min. Thus, C. viswanathii lipase can be successfully produced by an economic and sustainable process and advantageously applied for poultry fat hydrolysis without an additional acidification step to recover the released fatty acids.

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Section: Discussionmentioning

confidence: 99%

Agroindustrial Wastes as Alternative for Lipase Production by Candida viswanathii under Solid-State Cultivation: Purification, Biochemical Properties, and Its Potential for Poultry Fat Hydrolysis

et al. 2016

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“…In another study a Bacillus pumilus strain showed maximum xylanase production after 20 h using birchwood xylan (Duarte et al, 1999). Kapilan and Arasaratnam (2012) isolated B. subtilis from soil which exhibit highest xylanase activity after 42 h in SmF.…”

Section: Time Course Study For Xylanase Productionmentioning

confidence: 99%

Modulating the production of xylanase by Bacillus pumilus BS131 through optimization using waste fiber sludge

et al. 2023

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In recent days, cheapest alternative carbon source for fermentation purpose is desirable to minimize production cost. Xylanases have become attractive enzymes as their potential in bio-bleaching of pulp and paper industry. The objective of the present study was to identify the potential ability on the xylanase production by locally isolated Bacillus pumilus BS131 by using waste fiber sludge and wheat bran media under submerged fermentation. Culture growth conditions were optimized to obtain significant amount of xylanase. Maximum xylanase production was recorded after 72 hours of incubation at 30 °C and 7 pH with 4.0% substrate concentration. In the nutshell, the production of xylanase using inexpensive waste fiber sludge and wheat-bran as an alternative in place of expensive xylan substrate was more cost effective and environment friendly.

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Utilization of corncob xylan as a sole carbon source for the biosynthesis of endo-1,4-β xylanase from Aspergillus niger KIBGE-IB36

Javed

Aman

Qader

2017

Bioresour. Bioprocess.

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Comparison of the Kinetic Properties of Crude and Purified Xylanase from <i>Bacillus pumilus</i> with Commercial Xylanase from <i>Aspergillus niger</i>

Cited by 6 publications

References 11 publications

Agroindustrial Wastes as Alternative for Lipase Production by Candida viswanathii under Solid-State Cultivation: Purification, Biochemical Properties, and Its Potential for Poultry Fat Hydrolysis

Agroindustrial Wastes as Alternative for Lipase Production by Candida viswanathii under Solid-State Cultivation: Purification, Biochemical Properties, and Its Potential for Poultry Fat Hydrolysis

Modulating the production of xylanase by Bacillus pumilus BS131 through optimization using waste fiber sludge

Utilization of corncob xylan as a sole carbon source for the biosynthesis of endo-1,4-β xylanase from Aspergillus niger KIBGE-IB36

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