Hank’s balanced salt solution: an alternative resuspension medium to label autologous leukocytes. Experience in inflammatory bowel disease

Abstract

In this work Hank's balanced salt solution (HBSS) has been used, as resuspension medium, instead of leukocyte poor plasma (LPP) to label autologous white blood cells in 28 patients with suspicion af active inflammatory bowel disease.Labelled cells were reinjected and anterior and caudo-craneal views were obtained at 30 min, 2 h and 6 h p.i. Regions of interest were outlined on liver, spleen, lung, bone marrow (spine), background and lesions and the organ/background activity ratios were calculated in all scans. Patients were classified into 2 groups: Group 1: LPP, 30 patients and Groups 2: HBSS, 28 patients. Labelling efficiency was higher in HBBS group (89.0 ± 3.2 %) than in the LPP group (6.5 ± 6.3%). Organ/background activity ratios were similar in both groups. Concerning diagnostic accuracy was similar at 30 min and 2 h but the false positive rate increased at 6 h p.i. in the HBSS group. HBSS seems to be a valid alternative as resuspension medium in the labeling of autologous leukocytes but leukocyte poor plasma seem to induce less leukocyte damage. Based on these results, in our center HBSS is the currently used medium to label leukocytes.

Leukocyte-technetium-99m uptake in Crohn’s disease: Does it show subclinical disease?

“…The labeling of leukocytes with 99mTc-HMPAO was performed in accordance with the method described by Martin-Comin et al [13] . Briefly, blood samples (45 mL) were withdrawn from patients and healthy volunteers with a syringe that contained 6.0 mL anticoagulant [citric acidcitrate-dextrose (ACD)].…”

Technetium-99m ceftizoxime kit preparation

“…Contrary to conventional imaging techniques, imaging scintilography is based on physiological and biochemical alterations resulting from inflammatory and infectious processes. The radiolabeled leucocytes can be considered gold standard for the identification of inflammatory foci in the intestine and bone tissue Martin-Comin et al, 2002) However, the need to handle potentially contaminated blood can lead to the transmission of virus such as HIV and hepatitis B and C. In addition, the preparation of this radiopharmaceutical is laborious and requires specialized equipment, taking approximately three hours. The labeled leucocytes are not able to distinguish aseptic from septic inflammations.…”

Avaliação de processos inflamatórios na articulação temporomandibular empregando leucócitos autólogos marcados com tecnécio-99m em modelo animal

“…Este dado mostra que cerca de metade das moléculas do complexo 99m Tc-HMPAO que foram incubados com os leucócitos penetrou no seu interior marcando-os. Os resultados obtidos estão de acordo com os descritos por Martin-Comin et al (10) , que encontraram um valor médio de 55,3% a 75,4% de rendimento de marcação de leucócitos humanos.…”

“…O valor médio de lipofilicidade do HMPAO obtido neste estudo foi de 81%. Estudos na literatura demonstraram que, no momento da marcação dos leucócitos, a lipofilicidade do HMPAO era de 85% (8) e 78% (10) .…”

“…Neste estudo, após uma hora da administração endovenosa em coelhos, os leucócitos marcados apresentaram captação fisiológica pelo pulmão, fígado e baço, em concordância com vários trabalhos descritos na literatura (8,(10)(11)(12) . Este dado sugere que não tenha havido destruição dessas células durante o processo de marcação, ressaltando a integridade das mesmas.…”