LC50 of the Peptide Produced by the Entomopathogenic Fungus Nomuraea rileyi (Farlow) Samson Active Against Third Instar Larvae of Anticarsia gemmatalis (Lep.: Noctuidae)

Onofre, Sideney Becker; Gonzalez, Raul Riveros; Messias, Cassiano Gustavo; Azevedo, João Lúcio; Barros, Neiva Monteiro de

doi:10.1590/s1516-89132002000300004

Cited by 8 publications

(2 citation statements)

References 17 publications

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“…Wasti and Hartmann (1978) and Mohamed and Nelson (1984) have reported the toxic effect of N. rileyi mycelial extract toward 2nd instar larvae of Lymantria dispar via topical application and intra-haemocoelic injection and 3rd instar of Heliothis zea and via per os application against H. virescens, respectively. Recently, unidentified peptide was isolated from N. rileyi culture medium which showed insecticidal effect on 3rd instar A. gemmatalis after ingestion (Onofre et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Isolation of ergosterol peroxide from Nomuraea rileyi infected larvae of tobacco cutworm

Prompiboon

Bhumiratana²,

Ruchirawat³

et al. 2008

World J Microbiol Biotechnol

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In the search for potential cytotoxic substances produced by Nomuraea rileyi, an active compound was isolated from mycosed insects through an activity guided fractionation process. The compound, cytotoxic against the Sf9 insect cell line, was identified to be ergosterol peroxide (5a, 8a-epidioxy-24(R)-methylcholesta-6, 22-dien-3b-ol) using nuclear magnetic resonance techniques, infrared spectrometry, and mass spectroscopy. Anticancer screens demonstrated that ergosterol peroxide at micromolar concentrations inhibited the growth of hormone-dependent breast cancer cell line (T47D), hormone-independent breast cancer cell line (MDA-MB-231), human epidermoid carcinoma in mouth cell line (KB), human cervical carcinoma cell line (HeLa), lung cancer cell line (H69AR) and human cholangiocarcinoma cell line (HuCCA-1). Ergosterol peroxide showed moderate effects against Spodoptera litura larvae; 46.7% mortality via topical application after 7 day post-treatment whereas the insect's death was not found in per os application. The amounts of ergosterol peroxide produced by N. rileyi cultures under in vitro and in vivo were determined. The physiological levels of ergosterol peroxide detected in mycosed and mummified cadavers were very low (0.011 and 0.386 lg/larva) less then levels that either inhibited insect cell proliferation or caused insecticidal activity.

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Section: Introductionmentioning

confidence: 99%

Isolation of ergosterol peroxide from Nomuraea rileyi infected larvae of tobacco cutworm

Prompiboon

Bhumiratana²,

Ruchirawat³

et al. 2008

World J Microbiol Biotechnol

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“…Larvae of the gypsy moth, Lymantria dispar (L.), are killed either by spraying the culture fluid of N. rileyi on their body surface or by injecting the N. rileyi fluid into the hemocoel (Wasti & Hatmann, 1978). In addition, a peptide from the culture fluid of N. rileyi cultured for 12 days can kill third instars of Anticarsia gemmatalis Hübner (Onofre et al., 2002). These studies suggest that the culture fluid of N. rileyi has lethal effects on insects.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of phagocytic activity and nodulation in Galleria mellonella by the entomopathogenic fungus Nomuraea rileyi

Tseng

Tsai

et al. 2008

Entomologia Exp Applicata

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Changes in phagocytic activity and nodulation in the greater wax moth, Galleria mellonella (L.) (Lepidoptera: Pyralidae), were examined after treatment with the culture fluid of the entomopathogenic fungus Nomuraea rileyi SH1. When isolated hemocytes of G. mellonella were incubated with the conidia of N. rileyi in vitro, the rates of phagocytosis increased at 4 h after incubation but decreased subsequently. On the contrary, the rates of phagocytosis of isolated hemocytes decreased by 80% after 24 h preincubation with the fungal culture fluid (1/200, 1/100 dilutions). Levels of inhibition of phagocytic activity by the culture fluid depended on dilutions used. Galleria mellonella larvae showed a peak of nodulation at 4 h after injection with conidia. The percentage of nodules in hemolymph did not decrease by preinjection with the culture fluid, whereas the percentage of nodule‐containing conidia decreased, depending on the injected fluid. However, phagocytosis and nodulation in G. mellonella did not change after treatment of the culture fluid with proteinase K, indicating that the culture fluid contained proteinaceous immunosuppressive factors. Electrophoretic analysis of the culture fluid and not the fresh medium without culturing the fungus exhibited protein bands. Therefore, N. rileyi possibly secretes toxic proteins that impair cellular immune responses in G. mellonella larvae.

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Three in a Boat: Host-Plant, Insect Herbivore, and Fungal Entomopathogen

Applebaum

Ichelczik

Humber

2010

Cellular Origin, Life in Extreme Habitats and Astrobiology

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LC50 of the Peptide Produced by the Entomopathogenic Fungus Nomuraea rileyi (Farlow) Samson Active Against Third Instar Larvae of Anticarsia gemmatalis (Lep.: Noctuidae)

Cited by 8 publications

References 17 publications

Isolation of ergosterol peroxide from Nomuraea rileyi infected larvae of tobacco cutworm

Isolation of ergosterol peroxide from Nomuraea rileyi infected larvae of tobacco cutworm

Inhibition of phagocytic activity and nodulation in Galleria mellonella by the entomopathogenic fungus Nomuraea rileyi

Three in a Boat: Host-Plant, Insect Herbivore, and Fungal Entomopathogen

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