Isolation and characterization of selenate resistant mutants of Acremonium chrysogenum

Vialta, Airton; Catani, Cleide Ferreira; Renato, B.; Azevedo, João Lúcio de

doi:10.1590/s1516-89131999000300016

Cited by 3 publications

(2 citation statements)

References 7 publications

(13 reference statements)

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“…Selenate-resistant mutants with altered sulphate transport and assimilation have been characterized in several fungal genera, including Acremonium (Vialta et al 1999), Aspergillus (Arst 1968), Botrytis (Weeds et al 1998), Fusarium (Correll and Leslie 1987), Fig. 3 Heterokaryon formation between chromate-sensitive (chr-) and chromate-resistant (chr+) sulphate non-utilizing (sul) mutants of B. cinerea, and absence of heterokaryon formation when mutants were paired with themselves or with those carrying the same type of mutation.…”

Section: Discussionmentioning

confidence: 99%

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Vegetative compatibility grouping in Botrytis cinerea using sulphate non-utilizing mutants

2008

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Twenty-one strains of Botrytis cinerea isolated from six plant species on ten sites throughout Israel, as well as a strain from France, were tested for vegetative and mycelial incompatibility, pathogenicity, resistance to the fungicides carbendazim and iprodione, and colony morphology. Selenate-resistant mutants were isolated from the strains as spontaneous, fastgrowing sectors arising from restricted colonies on medium amended with sodium selenate with a mean frequency of 0.04 sectors/colony; 81% of the sectors were sulphate non-utilizing (sul) mutants. One hundred and four sul mutants were divided into two complementary groups: resistant (66 mutants) and sensitive to chromate. Based on compatibility reactions between chromate-resistant and chromate-sensitive sul mutants, 12 strains were compatible only with themselves and were each classified as belonging to different vegetative compatibility groups (VCGs). Nine strains were each compatible with one to three other strains, and were assembled into three multi-member VCGs. Mycelial incompatibility between wild-type strains (barrage), in the form of a zone of dark pigmentation or sparse mycelium with or without dark pigmentation of the agar along the line of confrontation, was observed for 70% of the inter-strain pairings. There was no correspondence in compatibility between strains revealed by two approaches: strains in different VCGs did not necessarily produce a barrage. However, self-compatibility was observed both as heterokaryon formation between complementary sul mutants and as an absence of barrages between mycelia of wild-type strains; wild-type strains belonging to the same VCG did not exhibit strong barrages, although weak antagonistic reactions were observed. Strains in two multimember VCGs showed the same patterns of resistance to carbendazim and iprodione; the third multi-member VCG contained isolates with different patterns of resistance. Four morphological types were revealed among wild-type strains: conidial (five strains), sclerotial (six strains), intermediate (ten strains), and mycelial (one strain). On bean leaves, conidial strains were more aggressive than sclerotial strains.

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Section: Discussionmentioning

confidence: 99%

“…Genetic variability in B. cinerea populations is very high, and the populations often resemble a mix of different strains without any correlation between the origins and properties of the strains (Alfonso et al 2000;Kerssies et al 1997;Vialta et al 1999). Vegetative compatibility provides an opportunity for population sub-structuring.…”

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Vegetative compatibility grouping in Botrytis cinerea using sulphate non-utilizing mutants

2008

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Vegetative Incompatibility in Botrytis

Korolev

Elad

2015

Botrytis – The Fungus, the Pathogen and Its Management in Agricultural Systems

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Use of Selenate-Resistant Strains as Markers for the Spread and Survival ofBotrytis cinereaUnder Greenhouse Conditions

Korolev¹,

Katan²,

Elad³

2006

Phytopathology®

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Botrytis cinerea marked strains combining traits of fungicide resistance or sensitivity (carbendazim, iprodione) with resistance to selenate were created and assessed for use in studying the dispersal of B. cinerea and its survival inside plant tissue under greenhouse conditions. Marked strains differed in their ability to cause lesions and to disperse in the greenhouse. A strain that was the most aggressive in infecting plants was also the most successful in spreading across the greenhouse. Following 7 to 14 days of exposure to marked inoculum, about 90% of plants showed quiescent B. cinerea infection with no significant difference between hosts or seasons. However, in a warm season, most of the plants were infected with wild-type B. cinerea, whereas most of the winter-recovered B. cinerea strains were of the marked phenotype, showing the importance of local inoculum from within the glasshouse in winter. The air of the greenhouse contained the same population of marked B. cinerea in warm and in cold periods, whereas the total population was significantly higher in summer. In the warm season, mycelium of B. cinerea inside plant debris lost viability within 3 to 4 months, whereas it stayed viable for 4 months in the winter (December to March) and started to lose viability in April.

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Isolation and characterization of selenate resistant mutants of Acremonium chrysogenum

Abstract: ABSTRACT

Cited by 3 publications

References 7 publications

Vegetative compatibility grouping in Botrytis cinerea using sulphate non-utilizing mutants

Vegetative compatibility grouping in Botrytis cinerea using sulphate non-utilizing mutants

Vegetative Incompatibility in Botrytis

Use of Selenate-Resistant Strains as Markers for the Spread and Survival ofBotrytis cinereaUnder Greenhouse Conditions

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