Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca

Morais, Glaucia Almeida de; Takaki, Massanori

doi:10.1590/s1516-89131998000100003

Cited by 7 publications

(4 citation statements)

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“…Unless there is a specific substrate to measure α- or β-amylase activity, it is necessary to inactivate one of the enzymes, especially in crude extracts. The commonly used procedures are based on selective inactivation of α-amylase by EDTA ( , ) or inactivation of β-amylase by thermal treatment or HgCl 2 ( , ). The specificity of the thermal treatment is questionable, because there are heat labile α-amylases from some plant species which could be inactivated by heat ( , ).…”

Section: Resultsmentioning

confidence: 99%

“…EDTA has been largely used as an α-amylase inhibitor by chelating the calcium present in the enzyme molecules altering their stability ( , ), and HgCl 2 treatments have been used for β-amylase inactivation, but there are controversies about the specificity and efficiency of these inhibitors ( , ).…”

Section: Resultsmentioning

confidence: 99%

“…Thermal treatment (70 °C/15 min) was developed for cereal amylases, and its effect on R-amylase activity from other sources, such as fruits, is unknown. Literature reports indicate that R-amylase activity in bean (26), wheat (31), and corn (32) extracts were reduced by 80 to 100% after heat treatment. On the other hand, MacGregor et al (33) and Doehlert and Duke (34) related a total inactivation of β-amylase through heat treatment in barley extracts, with no alteration of the R-amylase properties.…”

Section: Resultsmentioning

confidence: 99%

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Amylolytic Activity in Fruits: Comparison of Different Substrates and Methods Using Banana as Model

Bassinello

Cordenunsi

Lajolo

2002

J. Agric. Food Chem.

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Several methodologies have been developed for cereal amylolytic activity estimation, but there is lack of information about the application of these methods for fruits. Mature green banana fruit can achieve 20% of starch content which is degraded during the ripening period in a complex process involving alpha- and beta-amylases and alpha-1,4 and alpha-1,6-glucosidases, besides phosphorylases that can compete for the same substrates. Methods used to determine total hydrolytic activity and individual activity of enzymes involved in starch breakdown were compared for banana extracts in several ripening stages. Total hydrolytic activity was measured by DNS and iodometric methods. Endoamylolytic activity on amylose-azure substrate was also evaluated. BPNPG7 and PNPG5 chromogenic substrates were used for alpha- and beta-amylase activities, respectively. The results showed that methods that depend on the use of thermal treatment or on inhibitors to inactivate one of the enzymes were not adequate. The use of p-nitrophenol derivatives seemed to be the most specific, reproducible, and easiest method employed for single alpha- and beta-amylases activities determination in complex tissues. The DNS and iodometric methods can be used only for initial screenings of total hydrolytic activity, because the nonspecific substrate used in these procedures allows the action of more than one enzyme simultaneously.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Amylolytic Activity in Fruits: Comparison of Different Substrates and Methods Using Banana as Model

Bassinello

Cordenunsi

Lajolo

2002

J. Agric. Food Chem.

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“…Thirteen milliliters of distilled water were added to the samples, and absorbance changes were recorded at 620 nm. One unit of amylase activity was identified as the quantity of enzyme that caused the changes in the absorbance of 0.1 [ 35 ].…”

Section: Methodsmentioning

confidence: 99%

Progesterone Promotes Mitochondrial Respiration at the Biochemical and Molecular Level in Germinating Maize Seeds

Turk

2021

Plants

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This research aimed to investigate the effects of progesterone, a mammalian steroid sex hormone, on the mitochondrial respiration in germinating maize seeds. For this purpose, maize seeds were divided into four different groups (control, 10−6, 10−8, and 10−10 mol·L−1 progesterone) and were grown in a germination cabinet in the dark at 24.5 ± 0.5 °C for 4 d. The changes in gene expression levels of citrate synthase (CS), cytochrome oxidase (COX19), pyruvate dehydrogenase (Pdh1), and ATP synthase (ATP6), which is involved in mitochondrial respiration, were studied in root and cotyledon tissues. Significant increases were recorded in the gene expression levels of all studied enzymes. In addition, progesterone applications stimulated activities of malate synthase (MS), isocitrate lyase (ICL), and alpha-amylase, which are important enzymes of the germination step. The changes in gene expression levels of mas1 and icl1 were found parallel to the rise in these enzymes’ activities. It was determined similar increases in root and coleoptile lengths and total soluble protein and total carbohydrate contents. The most remarkable changes were detected in 10−8 mol·L−1 progesterone-treated seedlings. These results clearly indicate that progesterone stimulates mitochondrial respiration by inducing biochemical and molecular parameters and thus accelerates seed germination thanks to the activation of other pathways related to mitochondrial respiration.

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The use of starch azure for measurement of alpha-amylase activity

Lehoczki

Kandra

Gyémánt

2018

Carbohydrate Polymers

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Determination of amylase activity in cotyledons of Phaseolus vulgaris L. cv. carioca

Cited by 7 publications

References 0 publications

Amylolytic Activity in Fruits: Comparison of Different Substrates and Methods Using Banana as Model

Amylolytic Activity in Fruits: Comparison of Different Substrates and Methods Using Banana as Model

Progesterone Promotes Mitochondrial Respiration at the Biochemical and Molecular Level in Germinating Maize Seeds

The use of starch azure for measurement of alpha-amylase activity

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