Effects of vitamin C and E dietary supplementation on erythrocyte parameters of layer chickens reared in high ambient temperature and humidity

Abstract

A 30-day experiment involving 720 White Leghorn (L33) layer chickens of 39 weeks of age and average live weight of 1.8 ± 0.04 kg was carried out in a completely randomized design to evaluate the effects of vitamins C and E on erythrocyte parameters of layers during natural summer conditions. Birds were allotted to 4 treatments containing 0, 150 mg vitamin C, 150 mg vitamin E, and 150 mg vitamin C plus 150 mg vitamin E/kg feed. Each treatment was replicated four times. The exposure of layers to 31±3 °C and 33±0 °C ambient temperature and 84.6% and 81.5% relative humidity, inside and outside the experimental pen, during the study period caused an increase in temperature humidity index 15.5 above the threshold value of 70 established for this species. Total erythrocytes counts were not affected by treatment. However, hematocrit and hemoglobin were significantly (p<0.001) different in vitamin-C and E treated groups, individually or in combination, as compared to the control birds. Also, mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration of the birds fed vitamins C or E, or vitamins C+E were significantly (p<0.001) higher than those in control group. The results of the present study showed that the dietary supplementation of vitamin C and E, particularly as a combination, alleviated the counterproductive effects of high ambient temperature and humidity on the birds.

EFFECT OF Α-Tocopherol AND ASCORBIC ACIDS ON PERFORMANCE AND BLOOD IMMUNITY PROFILE OF MALE NATIVE MUSCOVY DUCK

“…α-Tocopherol supplementation is very effective for poultry because this may reduce the negative effects of corticosterone induced stress (Puthpongsiriporn et al, 2001). α-Tocopherol and ascorbic acid supplementations in diet, particularly in a combination, alleviates the counterproductive effects of high ambient temperature and humidity on the birds (Ajakaiye et al, 2010). They are protective agent that deactivates reactive oxygen species (ROS) and significantly prevent oxidative damage (Ramnath et al, 2008;Prisyanto et al, 2014).…”

Effect of Restricted Feeding on Growth Performance, Carcass Traits, Blood Hematology, Internal Organs, and Intestinal Histomorphology of Broilers

“…In addition, the number of heterophils and lymphocytes, and consequently the ratio of heterophils to lymphocytes, can vary depending on the condition (Simitzis et al, 2012). There are consistent reports of increased H/L ratios in stressed poultry species (Ajakaiye et al, 2010;Osti et al, 2017;Scanes, 2016). According Emadi and Kermanshahi (2006) the level of body resistance in poultry can be determined by the value of the H/L ratio, around 0.2 (low), 0.5 (normal) and 0.8 (high) for environmental adaptation.…”

The Effect of Quercus infectoria Acorn on Clinical, Hematobiochemical Parameters of Pregnant Albino Rats

“…Hematology (Hemoglobin (Hb) concentration, packed cell volume (PCV), white blood cells (WBCs) and red blood cells (RBCs) of the samples were performed as descried by (Nadeem et al, 2021) and differential leucocytic counts (DLC) by using Giemsa-stained blood smear technique (Gupte, 2009). Mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were calculated as stated by (Ajakaiye et al, 2010).…”