Avaliação do Metabolismo Nutricional em Poedeiras pela Técnica dos Isótopos Estáveis do Ccarbono (13C/12C)

Carrijo, Alfredo Sampaio; Pezzato, Antônio Celso; Ducatti, Carlos

doi:10.1590/s1516-635x2000000300003

Cited by 12 publications

(8 citation statements)

References 12 publications

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“…Segundo Libby et al (1964), tecidos metabolicamente mais ativos, como o fígado, o pâncreas e o tecido gorduroso têm taxa de substituição mais rápida que os menos ativos, como os ossos e o tecido conjuntivo. Carrijo et al (2000) observaram essas evidências quando avaliou o turnover de diferentes tecidos de poedeiras comerciais pela técnica dos isótopos estáveis ( 13 C/ 12 C).…”

Section: Resultsunclassified

Deposição de ácido linoléico conjugado (CLA) em tilápias-do-nilo

Santos

Furuya²,

Matsushita³

et al. 2007

R. Bras. Zootec.

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RESUMO -Objetivou-se avaliar a deposição de ácido linoléico conjugado (CLA) em filés de tilápia-do-nilo (Oreochromis niloticus). Foram utilizados peixes com 121,08 ± 8,48 g, distribuídos em quatro caixas de cimento amianto (1,0 m 3 cada uma) e arraçoados até saciedade aparente, duas vezes ao dia, durante 49 dias. Como alimento, utilizou-se ração comercial extrusada com 28% de PB e 3.000 kcal de energia digestível/kg, acrescida de 2% do produto comercial Luta-CLA ® (BASF -Brasil). No início do experimento, cinco peixes foram coletados aleatoriamente como amostra controle (sem CLA). A cada sete dias, dois peixes de cada caixa foram coletados, pesados e filetados para determinação da taxa de deposição de CLA nos filés. As amostras de filés foram estocadas a -24 o C para análise do teor de lipídios totais e do perfil de ácidos graxos. A taxa de deposição de CLA foi determinada pela função de Gompertz (y = a . exp{-b . exp[-kt]}). O meio-tempo (T) para deposição de 50% do CLA foi estimado pela equação T = ln2/k, em que: T representa o meio-tempo; ln , o logaritmo Neperiano e k, a unidade de tempo que fornece a "velocidade" de deposição do CLA no tecido muscular da tilápia-do-nilo. A deposição máxima de CLA nos lipídios totais do filé de tilápias-do-nilo estimada pela função de Gompertz foi de 1,61 g/100 g de lipídios do filé. O meio-tempo de deposição de CLA nos lipídios do filé ocorre com 6,86 dias. Palavras-chave: ácidos graxos, ácido linoléico conjugado, Oreochromis niloticus Deposition of conjugated linoleic acid (CLA) in Nile tilapiaABSTRACT -This study was carried out to determine the deposition of conjugated linoleic acid (CLA) in Nile tilapia Oreochromis niloticus fillet. Fish averaging 121.08 ± 8.48 g were distributed in four cement amianthus tank (1.0 m 3 each) and hand fed to apparent satiation, two times a day for 49 days. As feed, an extruded commercial diet with 28% CP and 3000 kcal of digestible energy was utilized. A commercial CLA product (Luta-CLA ® , BASF) was added at 2% of the diet. At the beginning of the experiment, five fish were randomly collected as a control sample (without CLA). Weekly, two fish of each tank were collected, weighed and filleted for measurement of CLA deposition ratio in the fillets. The fillet samples were stored at -24 o C for total lipid and analyses of fatty acids profiles. The deposition rate of CLA was determined by Gompertz function (y = a . exp{-b . exp[-kt]}). Half time (T) to have 50% deposition of CLA was calculated by the equation T = ln2/k, where T is half time; ln is Napierian logarithm and constant k the time unit, which provides the "velocity" in the deposition of CLA in the Nile tilapia fillets. The maximum CLA deposition in the fish fillet lipids, estimated by the Gompertz function, was 1.61 g/100 g fillets lipids. Half time of CLA deposition in the fillet lipids occur within 6.86 days.Key Words: conjugated linoleic acid, fatty acids, Oreochromis niloticus IntroduçãoEntre os setores da produção animal, a aqüicultura é o que se expande mais rapidamente. A t...

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Section: Resultsunclassified

Deposição de ácido linoléico conjugado (CLA) em tilápias-do-nilo

Santos

Furuya²,

Matsushita³

et al. 2007

R. Bras. Zootec.

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“…The abundance of naturally marked substrates, with no restriction to its use in terms of environmental contamination, indicates that these tracers can be used in studies on aquatic organisms (Schroeder, 1983), sharks (Domi et al, 2005), oysters (Piola et al, 2005), and seals (Zhao et al, 2006). The team of the Center of Stable Environmental Isotopes (CIE/IB/UNESP Botucatu) suggests that these natural tracers can be successfully used in studies on layers (Denadai et al, 2007;Carrijo et al, 2000), broilers (Gottmann, 2007;Oliveira, 2005;Carrijo et al, 2006;Cruz, 2002), fish (Zuanon et al, 2006), and sheep (Silva, 2003).…”

Section: Introductionmentioning

confidence: 99%

Poultry offal meal traceability in meat quail tissues using the technique of stable carbon (13C/12C) and nitrogen (15N/14N) isotopes

Móri

Garcia

Ducatti

et al. 2008

Rev. Bras. Cienc. Avic.

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Studies on the detection of animal by-products in poultry meat are rare, and non-existent on quail meat. This study aimed at detectiong increasing levels of poultry offal meal (POM) in quail meat, using carbon (13C/12C) and nitrogen (15N/14N) stable isotopes technique. Sixty four on-day-old male quails derived from a commercial farm were randomly distributed into seven different groups, which were fed experimental diets containing 0, 1.5, 3.0, 4.5, 6.0, 7.5, and 15% of POM. Diets were formulated to contain equal energy, protein, and amino acid levels. Four individuals per treatment were sacrificed at 42 days of age for breast muscle (Pectoralis major), keel, and tibia collection, which were subsequently submitted to analyses. Isotopic δ13C and δ15N enrichment was observed in all analyzed tissues, with the lowest detection level of 3% dietary inclusion of poultry offal meal

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“…The dehydrated samples were obtained by drying the samples in a forcedventilation oven (Marconi, model MA 035, SP, Brazil) at 56ºC for 48 hours or freeze-drying in a lyophilizer (model L108, Liobras ® , SP, Brazil). The duration of the lyophilization process was 48 hours and the samples were dried under vacuum at -55°C and pressure around 50 μHg, and then cryogenically ground (Spex-6750 freezer-mill, Metuchen, USA) at -196ºC (Carrijo et al, 2000;Denadai et al, 2008).…”

Section: Methodsmentioning

confidence: 99%

“…Fat was extracted from the dehydrated and cryogenically-ground samples in a Soxhlet apparatus in the ether treatment (Carrijo et al, 2000;Denadai et al, 2008) and according to the method of Folch et al (1957) in the chloroform + methanol treatment.…”

Section: Methodsmentioning

confidence: 99%

Standardization of A Sample-Processing Methodology for Stable Isotope Studies in Poultry

Denadai

Sartori

Pezzato

et al. 2019

Braz. J. Poult. Sci.

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The objective of this study was to determine if lipid extraction processes alter the isotopic value of δ 13 C and δ 15 N of tissues (pectoral muscle, thigh and liver) and eggs and if the use of anticoagulants interferes with blood and plasma δ 13 C and δ 15 N isotopic values. Samples were acquired from the same flock of birds. The 32 egg samples were randomly divided into four treatments (liquid, dehydrated, and fat-extracted with ether or chloroform + methanol) with eight replicates each. The 24 samples of pectoral muscle, thigh muscle and liver of broilers were randomly divided into three treatments (dehydrated, fat-extracted with ether and chloroform + methanol) with eight replicates each. Blood samples were divided into a 3x3 factorial arrangement with three physical forms (liquid, oven-dried or freeze-dried) and three collection methods (with no anticoagulant, with EDTA or heparin). Plasma samples were distributed in a 3x2 factorial arrangement, with three physical forms (liquid, ovendried, or freeze-dried) and two anticoagulants (EDTA or heparin). The obtained isotopic results were submitted to the multivariate analysis of variance (MANOVA) and univariate (ANOVA, complemented by Tukey' test), using the GLM procedure of the statistical program SAS (1996) or Minitab 16. The results show that it is possible to use the evaluated methods of fat extraction, drying and anticoagulants in the isotopic analyses of carbon-13 and nitrogen-15 in chicken tissues.

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Avaliação do Metabolismo Nutricional em Poedeiras pela Técnica dos Isótopos Estáveis do Ccarbono (13C/12C)

Cited by 12 publications

References 12 publications

Deposição de ácido linoléico conjugado (CLA) em tilápias-do-nilo

Deposição de ácido linoléico conjugado (CLA) em tilápias-do-nilo

Poultry offal meal traceability in meat quail tissues using the technique of stable carbon (13C/12C) and nitrogen (15N/14N) isotopes

Standardization of A Sample-Processing Methodology for Stable Isotope Studies in Poultry

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