Evaluation of sodium sulfite and protein correction in analyses of fibrous compounds in tropical forages

Abstract

-The objective of this study was to evaluate the contents of fibrous compounds in tropical grasses and legumes according to utilization of sodium sulfite in the neutral detergent solution or using a procedure for contaminant protein correction. Samples of ten grasses and ten legumes were used. The contents of neutral detergent fiber were decreased when sodium sulfite was used; however, more prominent reductions were verified in legumes. Sodium sulfite decreased the acid detergent fiber content in both forage groups. The contents of neutral and acid detergent insoluble protein and lignin were reduced by sodium sulfite in legumes, but no effect was observed in grasses with regard to these variables. The decrease in fiber contents in legumes could be explained by the solubilization of lignin and decrease in insoluble nitrogen. However, the decreases in fiber in grasses could not be solely explained by the decrease in contaminant protein and solubilization of lignin, and loss of other fibrous compounds probably occurred. The utilization of sodium sulfite compromises the accuracy of the estimates of fibrous compounds contents in tropical forages. The precision of the estimates were not relevantly increased by sodium sulfite. The correction of insoluble fibrous compounds for protein is suggested instead of using sodium sulfite because there are no modifications on neutral detergent solution or undesirable solubilization of fibrous compounds.Key Words: acid detergent fiber, acid detergent insoluble protein, lignin, neutral detergent fiber, neutral detergent insoluble protein Revista Brasileira de Zootecnia

Effect of soya bean oil supplementation and forage type on methane production and fibre digestibility using the in vitro gas production system

“…Xara es without N fertilization (Campos et al, 2016). It is important to note that these authors did not use sodium sulphite in NDF analysis, which can explain the lower NDF value observed in our study (Gomes et al, 2012). Thus, our tropical grass samples have compositions that are consistent with their type, albeit under specific conditions.…”

Black rhinoceros (Diceros bicornis) and domestic horse (Equus caballus) hindgut microflora demonstrate similar fermentation responses to grape seed extract supplementation in vitro

“…Laurel, NJ, USA; AOAC, , 990.03). Due to a lack of scientific consensus on sodium sulphite (SS) inclusion for detergent fibre determination in CT‐containing samples (Pagán et al., ; Gomes et al., ), the effect of SS on sample NDF and ADF concentration was tested. Diet and effluent samples were analysed for NDF following the methods of Van Soest et al.…”

Métodos utilizados para determinação dos constituintes da fração fibrosa, uma revisão

“…Já Mertens (2002), cita que o sulfito de sódio não solubiliza a lignina e seu emprego é importante para remoção das contaminações por proteína. Gomes et al (2012), ao avaliar os efeitos do uso de sulfito de sódio em solução de detergente neutro na estimativa de compostos fibrosos em gramíneas e leguminosas tropicais. Observaram que o teor de FDN diminuiu quando foi utilizado sulfito de sódio, com maior redução nas leguminosas.…”

“…No caso do método da fibra bruta não existe um método padrão específico, somente um método referência recomendado pela AOAC, e o mesmo foi desenvolvido e melhorado gradativamente com base em um método empírico. Portanto, o método de determinação das frações fibrosas ainda se mantém empírico, e todas as etapas analíticas detalhadas por Mertens (2002) assim como qualquer modificação do método pode afetar o METHODS USED TO DETERMINATION THES CONSTITUENTS OF THE FIBROUS FRACTION, A REVIEW que se mede como fibra (Gomes et al, 2012;Da Silva et al, 2018). Desta forma, os resultados provenientes do sistema detergente dependem rigorosamente das recomendações descritas nos protocolos.…”

“…A fibra é um termo nutricional, que pode ser somente determinada durante o processo digestivo e sua definição está vinculada à sua origem e a metodologia analítica utilizada na sua determinação laboratorial (Mertens, 2003;Gomes et al, 2012). Resistente à digestão enzimática, a fibra acaba servindo de substrato para atividade fermentativa (Van Soest et al, 1991;Jung, 1997), por apresentar digestibilidade variável, ocupa METHODS USED TO DETERMINATION THES CONSTITUENTS OF THE FIBROUS FRACTION, A REVIEW espaço no trato gastrointestinal podendo limitar o consumo dos animais (Jung, 1997;Mertens, 2003;Geron, et al 2014).…”