Efeito de diferentes relações treonina: lisina digestíveis, suplementadas ou não com glicina, sobre a atividade enzimática em pintos de corte

Abstract

RESUMO -O experimento foi conduzido com o objetivo de avaliar o efeito da suplementação de glicina em dietas com diferentes relações treonina:lisina digestíveis sobre a atividade de enzimas que metabolizam a treonina. Foram utilizados 560 pintos de corte machos, linhagem Cobb, durante o período de 8 a 21 dias de idade, distribuídos em delineamento inteiramente casualizado, num arranjo fatorial 3 × 2 + 1, composto de três relações treonina:lisina digestíveis (55; 65 e 75%), com suplementação ou não de glicina, mais um tratamento adicional, contendo farinha de carne e ossos e com relação treonina:lisina digestíveis de 65%. Utilizaram-se oito repetições por tratamento e 10 aves por unidade experimental alojadas em baterias metálicas. Procedeu-se à coleta total de excretas durante toda a fase experimental. Para determinação da atividade das enzimas treonina aldolase, treonina desidrogenase e treonina desidratase, foram abatidas no final do experimento duas aves por unidade experimental para retirada do fígado. As relações treonina:lisina influenciaram a atividade das três enzimas no fígado: a maior atividade de treonina aldolase foi para a relação de 75%; a de treonina desidrogenase para a relação de 65%, e a de treonina desidratase para a relação de 55%. A suplementação de glicina reduz a atividade de todas as enzimas avaliadas.Palavras-chave: treonina aldolase, treonina desidratase, treonina desidrogenase Effect of different digestible threonine:digestible lysine ratios, with or without glycine supplementation, on the enzymatic activity in broiler chicks ABSTRACT -The experiment was conducted with the objective evaluating the effect of supplementation of glycine in diets with different digestible threonine:digestible lysine ratios on the activity of enzymes that metabolize threonine. Five hundred and sixty male broiler chicks from the Cobb strain were used during the period of 8 to 21 days, distributed in a completely randomized design, in a 3 × 2 + 1 factorial arrangement, with 3 digestible threonine:digestible lysine ratios (55; 65 and 75%), with or without supplementation of glycine, plus an additional treatment, containing meat and bone meal, with a 65% digestible threonine:digestible lysine ratio. Eight repetitions were used per treatment and 10 birds per experimental unit, allotted in metal battery cages. The total excreta collection was performed throughout the whole experimental stage. For the determination of the activity of the threonine aldolase, threonine dehydrogenase and threonine dehydratase enzymes, at the end of the experiment, two birds were slaughtered and had their livers extracted. The threonine:lysine ratios influenced the activity of the three enzymes in the liver; while the greatest activity of the threonine aldolase was for the ratio of 75%, for threonine dehydrogenase it was 65%, and for the threonine dehydratase it was 55%. Supplementation of glycine reduced the activity of all enzymes evaluated.

<b>A procedure to evaluate the efficiency of utilization of dietary amino acid for poultry

“…These values were close to those found for females by Silva Junior et al (2006) and Nascimento et al (2009b) with female slow-growing broilers, which obtained values from 77 to 82 %, respectively. For the ratio Thr: Lys, the results were similar to those registered in recent studies (BERNARDINO et al, 2011;DUARTE et al, 2012). Thus, the methodology employed shown to be applicable to assess the efficiency of amino acid utilization, since it allowed obtaining results that were supported by recent findings in the literature.…”