Incidence of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in patients with urinary tract infection

Ghafourian, Sobhan; Neela, Vasanthakumari; Khosravi, Afra; Rahbar, Mohammad; Sadeghifard, Nourkhoda

doi:10.1590/s1516-31802012000100007

Cited by 25 publications

(15 citation statements)

References 18 publications

(13 reference statements)

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“…In our present study, two isolates (K1 and K2) of K.pneumoniae had plasmid molecular weight of 982 kb. This plasmid likely to bear high antibiotic resistance encoding beta-lactamse enzyme confer resistance to ampicilin, kanamycin, chloramphenicol [35,36]. Our present study found majority of isolates (80%) resistant to antibiotic ampicilin, 70% resistant to chloramphenicol and kanamycin.…”

Section: In -Vitro Assay For Virulence Factorssupporting

confidence: 53%

Targeting of Virulence Factors and Plasmid Profiling of Klebsiella pneumoniae Causing Urinary Tract Infection in Sylhet City of Bangladesh

Foysal

Majlish

Islam

et al. 2018

Braz. arch. biol. technol.

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Studies were conducted to characterize Klebsiella pneumoniae isolates from urinary tract infection (UTI) patients in Sylhet city of Bangladesh. At the same time, all isolates were screened for some common virulence genes and four significant isolates were searched for plasmid number and sizes by mini alkaline-lysis method. Among five tested isolates from female UTI patients, gyrase subunit B2 (gyrb2) amplified in all isolates, lipase and nuclease detected in three isolates and serine protease amplifies in two isolates and gave the expected band of 1130 bp, 517 bp, 1055 bp and 211 bp respectively. Two of four isolates showed 9.82 kb plasmid band on agarose gel. Isolates bearing 9.82 kb plasmid were found to be resistant to multiple commercial antibiotics. At the same time all isolates were screened for in-vitro plate assay for proteolytic, lypolytic and hemolytic activity. Isolates with positive plasmid and more than one virulent gene with gyrB2 showed positive result in in-vitro culture plate with clear zone of proteolysis, hemolysis or lipolysis. This study will be helpful for further study in finding correlation or pattern of virulence properties for K. pneumoniae associated UTI in Bangladesh.

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Section: In -Vitro Assay For Virulence Factorssupporting

confidence: 53%

Targeting of Virulence Factors and Plasmid Profiling of Klebsiella pneumoniae Causing Urinary Tract Infection in Sylhet City of Bangladesh

Foysal

Majlish

Islam

et al. 2018

Braz. arch. biol. technol.

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“…Previously, the rates of bla SHV containing Enterobacterales with a focus on uropathogenic isolates were reported 12.2% to 54.8% by Iranian authors. [24][25][26][27][28][29] Meanwhile, several authors have shown the global spared of SHV type β-lactamases in uro- pathogenic Enterobacterales from China, 30 India, 31 Saudi Arabia, 32 Turkey, 33 Ukraine, 34 and Morocco. 35 In our results, 4.1% of uropathogenic Enterobacterales contained bla NDM-1, which to the best of our knowledge, is the first prevalence of this gene in Guilan province (North of Iran).…”

Section: Discussionmentioning

confidence: 99%

The Phylogenetic Relatedness of blaNDM-1 Harboring Extended-Spectrum β-Lactamase Producing Uropathogenic Escherichia coli and Klebsiella pneumoniae in the North of Iran

Jamali¹,

Tavakoly²,

Mojtahedi³

et al. 2020

IDR

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Background: Escherichia coli and Klebsiella pneumoniae as an important part of Enterobacterales family are important causes of both community-and hospital-acquired infections. The present study aimed to investigate the prevalence of antibiotics resistance and molecular characteristics of uropathogenic isolates of E. coli and K. pneumoniae in Iranian patients. Methods: This cross-sectional study performed on 223 Escherichia coli and 68 Klebsiella pneumoniae isolates obtained from hospitalized patients in the north of Iran. The isolates were identified by standard microbiologic tests and confirmed by API 20E strip. Disk diffusion method was applied to determine antibiotic susceptibility pattern. The presence of β-lactamases encoding genes was evaluated by PCR method. Analysis of the mutations and homology among sequences was done by the CLC sequence viewer (Qiagen, Denmark), and phylogenetic trees were constructed by the neighbor-joining method (Bootstrap: 1000 times). Results: The overall rates of extended-spectrum β-lactamases (ESBLs)-producing E. coli and K. pneumoniae isolates were 37.7% and 32.4%, respectively. The overall presence of bla SHV , bla NDM-1 , and bla OXA-1 genes was detected in 16 (5.5%), 12 (4.1%), and 48 (16.4%) of isolates, respectively. The neighbor-joining analysis for E. coli KU985246.1 strain showed that the most related bla NDM-1 sequences were from China, Singapore, UK, Thailand, and Bangladesh. While K. pneumoniae KU985245.1 strains were mostly related to bla NDM-1 sequences form Myanmar, and China. Conclusion: In summary, the remarkable rate of ESBL-producing uropathogenic Enterobacterales along with the first prevalence of NDM-1 β-lactamases can be a serious concern in our region.

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“…The high prevalence of ESBL producing E. coli isolates from outpatients calls for the need to aware of this increasing resistance between uropathogens and update bacterial susceptibility data and to set up our empirical therapy accordingly. [9].Treatment of infections caused by ESBLproducing bacteria has become a world problem and has led to an increase in the death of patients, as well as a greater financial burden for treatment [10]. Because of rising antibiotic resistance between organisms and the high prevalence of ESBL producing E. coli in the community and its importance in managing UTIs, local hospital-based knowledge of the uropathogens and their antibiotic susceptibility pattern is necessary and helpful for physicians to choose appropriate empirical treatment [11,12].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Antibiotic Susceptibility Pattern of Extended Spectrum Beta Lactamases Producing Escherichia Coli Isolated from Outpatients with Urinary Tract Infections in Babol, Northern of Iran

Baziboroun

Bayani

Poormontaseri

et al. 2018

Current Issues in Pharmacy and Medical Sciences

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Received 08 November 2017 Accepted 15 February 2018 The incidence of community-acquired UTIs due to extended-spectrum beta-lactamase (ESBL) producing E. coli isolates, has increased worldwide and is considered a great problem in the treatment of infections. The aim of this study was to determine the prevalence of ESBL producing E. coli isolates in urine samples of outpatients in Babol, North of Iran. A total of 3 699 urine samples from outpatients referred to Yahyanejad Hospital, Babol, Iran, were collected during 15 months, from March 2016 to June 2017. The samples were processed for bacterial culture using conventional methods, while antimicrobial susceptibility testing for E. coli isolates was performed by the disc diffusion method. The prevalence of ESBL producing E. coli isolates were assessed by Double-Disc tests. Of 3699 tested sample, 201 samples showed the growth of pathogens and among them, 106 isolates (52/7%) were E. coli. The rate of ESBL producing E. coli isolates was 25/4%.The majority (81/5%) of the isolates were from females. High percent resistance was found against ceftazidime, cefotaxime, ceftriaxone, cefixime and ciprofloxacin, ranging from 61-100%, while the highest percent susceptibility was seen to meropenem, piperacillin- tazobactam (100%), followed by nitrofurantoin and amikacin (91%). The high prevalence of ESBL producing E. coli isolates from outpatients calls for the need to aware of this increasing resistance between uropathogens and update bacterial susceptibility data and to set up our empirical therapy accordingly.

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Incidence of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in patients with urinary tract infection

Cited by 25 publications

References 18 publications

Targeting of Virulence Factors and Plasmid Profiling of Klebsiella pneumoniae Causing Urinary Tract Infection in Sylhet City of Bangladesh

Targeting of Virulence Factors and Plasmid Profiling of Klebsiella pneumoniae Causing Urinary Tract Infection in Sylhet City of Bangladesh

<p>The Phylogenetic Relatedness of <em>bla</em>N<sub>DM-1</sub> Harboring Extended-Spectrum β-Lactamase Producing Uropathogenic <em>Escherichia coli</em> and <em>Klebsiella pneumoniae</em> in the North of Iran</p>

Prevalence and Antibiotic Susceptibility Pattern of Extended Spectrum Beta Lactamases Producing Escherichia Coli Isolated from Outpatients with Urinary Tract Infections in Babol, Northern of Iran

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