Model of human epidermis reconstructed in vitro with keratinocytes and melanocytes on dead de-epidermized human dermis

Rehder, Jussara; Souto, Luís Ricardo Martinhão; Issa, Cláudia Maria Bernardino Magro; Puzzi, Maria Beatriz

doi:10.1590/s1516-31802004000100006

Cited by 28 publications

(19 citation statements)

References 13 publications

(23 reference statements)

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“…10 Since then, different models of reconstructed skin were developed, studied, and commercialized. 1,3,5,11,12 These models were characterized and compared by several techniques to natural skin, excised human skin, and animal model skin (rat and pig). 13,14 Among the techniques actually used to study the skin, Raman spectroscopy presents the advantages of being nondestructive, it also allows a real-time structural and molecular analysis, without requiring any sample preparation.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of reconstructed skin model by Raman microspectroscopy: Comparison with excised human skin

et al. 2007

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Human skin is directly exposed to different exogenous agents. Many research works have studied the diffusion, interactions, absorption mechanisms, and/or toxicity of these agents toward different cutaneous structures. With the use of living animals for such tests being more and more rejected; and the number of human volunteers being limited; different types of skin models are used. In the last few years, reconstructed epidermis from cell cultures has been frequently employed, and recent changes in the European chemical policy have approved and encouraged the use of these reconstructed models for skin‐related research works and assessments. Among the techniques used actually to study the skin, Raman microspectroscopy is a rising and powerful nondestructive technique that detects characteristic molecular vibrations. In this study, we created a spectral database to index the vibration peaks and bands of a well‐known reconstructed epidermis model, the Episkin®. The comparison with a native epidermis signal enabled us to put in evidence several spectral differences associated with molecular and structural differences between the skin and the reconstructed model, both maintained in living conditions. In addition to that, we have showed the feasibility of tracking the penetration of a pharmaceutical molecule through the Episkin model. © 2007 Wiley Periodicals, Inc. Biopolymers 87: 261–274, 2007.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

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Section: Introductionmentioning

confidence: 99%

Molecular characterization of reconstructed skin model by Raman microspectroscopy: Comparison with excised human skin

et al. 2007

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“…It is known that damaged skin is repaired through proliferation and growth of dermal cells (essentially fibroblasts) and/or epidermal cells (keratinocytes and melanocytes) (Jansson et al 2001) which should be cultivated and considered for the types of cell therapy proposed by tissue engineering with the purpose of healing ulcers (Kim et al 1999;Stock and Vacanti 2001;Margulis et al 2004;Souto et al 2006;Rehder et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Tissue therapy with autologous dermal and epidermal culture cells for diabetic foot ulcers

et al. 2011

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A great part of diabetic ulcers on the lower extremities have difficult healing and represent the most common cause of non-traumatic amputation In case of patients unresponsive to the classical therapy with debridement, dressings and systemic antibiotic therapy, cell therapy may be an excellent indication. The objective of this study was to assess the efficacy of autologous skin cell (fibroblasts and keratinocytes) implants cultivated ex vivo and applied to long-standing (9-34 years) skin ulcers of five diabetic patients (4 DM2 and 1 DM1) with autologous fibrin glue. There were six ulcers of onset between 4 months and 20 years before and from 4.0 to 36.62 cm(2) in size, located on the lower limbs and unresponsive to the several conventional treatments. Complete healing was observed in five ulcers (83.3%), after 21-120 days. The patient who presented the largest ulcer had partial improvement in 40 days. It is believed that the more distal ulcer location is, the worse is its prognosis. There probably is a correlation between healing time, ulcer size and prior duration. No adverse reactions derived from the treatment occurred. It is concluded that this method is an excellent therapeutic option for diabetic ulcers, allowing faster healing. Its great advantage is being a minimally invasive procedure that can be carried out in an outpatient clinic.

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“…As células basais se dividem para formar os queratinócitos, os quais são morfologicamente e bioquimicamente modificados durante o processo de queratinização ou diferenciação epidérmica. Este processo progride através das outras camadas da epiderme até a camada córnea (EDWARDS, 2005;REHDER et al, 2004).…”

Section: Revisão Da Literaturaunclassified

“…As raízes dos pêlos e das glândulas sebáceas prolongam-se até o tecido subcutâneo. É uma camada elástica e fornece nutrição para a epiderme e para os apêndices cutâneos (ABRAHAMS; CRAVEN; LUMLEY, 2005;EDWARDS, 2005;HABIF, 1996;REHDER et al, 2004).…”

Section: Dermeunclassified

Atividades cicatrizante e antimicrobiana do óleo-resina de copaíba (Copaifera langsdorffii) em úlceras cutâneas

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Model of human epidermis reconstructed in vitro with keratinocytes and melanocytes on dead de-epidermized human dermis

Abstract: It is possible to obtain a completely differentiated human epidermis reconstructed in vitro from keratinocyte and melanocyte cultures on a dead de-epidermized human dermis.

Cited by 28 publications

References 13 publications

Molecular characterization of reconstructed skin model by Raman microspectroscopy: Comparison with excised human skin

Molecular characterization of reconstructed skin model by Raman microspectroscopy: Comparison with excised human skin

Tissue therapy with autologous dermal and epidermal culture cells for diabetic foot ulcers

Atividades cicatrizante e antimicrobiana do óleo-resina de copaíba (Copaifera langsdorffii) em úlceras cutâneas

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