Enzymes and membrane proteins of ADSOL-preserved red blood cells

Leonart, Maria Sueli Soares; Nascimento, Aguinaldo José do; Nonoyama, Kimiyo; Pelissari, Cinthia Barbosa; Barretto, Orlando César de Oliveira

doi:10.1590/s1516-31802000000200003

Cited by 5 publications

(5 citation statements)

References 16 publications

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“…It is well known that during blood preservation, blood hydrogen ion concentration and potassium levels increase. [32][33][34][35] As expected, the pH decreased and potassium levels increased over time during storage in our study. Our findings for the pH and potassium are thus in agreement with previous reports.…”

Section: Discussionsupporting

confidence: 69%

Beneficial Effects of Melatonin on Oxidative Damage Observed During Whole Blood Storage.

Ozcelik¹

2014

UHOD

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It is well known that oxidative stress rises during the storing period of whole blood and there has been a great interest to improve the quality of stored blood for longer periods. In the present study, we determine the effects of melatonin as an antioxidant on the whole blood indices stored under blood bank conditions. Human blood was collected by venipuncture into citrate-dextrose-phosphate (CDP) bags from healthy volunteers. Immediately after collection, blood was subdivided into two parts and exposed to either melatonin or control saline solutions. Several biochemical parameters were measured on the day of collection and at weekly intervals up to 3 weeks. Mean corpuscular and platelet volumes tended to increase during storing process and with melatonin, these indices remained near to physiologic levels. As expected, acidity, oxidative damage and osmotic fragility increased in stored blood. Interestingly addition of melatonin reduced acidity, oxidative damage by lowering malondialdehyde levels and by increasing superoxide dismutase activity, and osmotic fragility during storage without adversely affecting other biochemical parameters. pH was 6.68 ±0.04 in day 14 of control group while it was 7.00 ± 0.03 in melatonin group. Malondialdehyde level was 1.223±0.05 in day 21 of control group while it was 0.723±0.04 in melatonin group (p< 0.05).Superoxide dismutase activity was significantly higher in melatonin group at days 14 and 21 (151.3±12.2 and 82.8±9.2) compared to saline (111.8±6.5 and 44.8±3.0) (p< 0.05). Through these results, we could confirm a central role for oxidative stress in the mechanism of the most evident blood storage lesions and melatonin seems to exhibit beneficial effects on these lesions. Keywords: Whole blood storage, Oxidative stress, Melatonin ÖZET Kanın Depolanmasında Görülen Oksidatif Hasarda Melatoninin Koruyucu EtkileriKanın depolanması sırasında oksidatif stresin arttığı iyi bilinmekte ve depo kanın kalitesinin daha uzun sürelerle korunması için yoğun çalışmalar yapılmaktadır. Bu çalışmada, kan bankası şartlarında saklanan tam kan örneklerinde bir antioksidan olan melatoninin etkisi araştırılmıştır. Sağlıklı gönüllü bir grup insandan venöz kan örnekleri, antikoagulanlı torbalara alınmıştır. Bağıştan hemen sonra kan, kontrol grubu ve melatonin grubu olarak iki gruba ayrılmış ve bağış gününde ve sonraki üç hafta boyunca her hafta çeşitli biyokimyasal analizler yapılmıştır. Ortalama eritrosit ve trombosit hacim değerleri saklama boyunca artmış, ancak melatoninle bu değerler normale yakın seyretmiştir. Beklendiği gibi, saklanan kanda asidite, ozmotik frajilite ve oksidatif hasar artmıştır. İlginç olarak, melatonin ilavesi asiditeyi, ozmotik frajiliteyi ve oksidatif hasarı (malondialdehit seviyelerini azaltıp superoksit dismutaz aktivitesini artırarak) anlamlı olarak düşürmüştür.Kontrol grubunda 14. gün pH değeri 6.68±0.04 iken, melatonin grubunda bu değer 7.00 ± 0.03 idi. Malondialdehit düzeyi kontrol grubunda

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Section: Discussionsupporting

confidence: 69%

Beneficial Effects of Melatonin on Oxidative Damage Observed During Whole Blood Storage.

Ozcelik¹

2014

UHOD

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“…Significant reductions in the RBC GSH content (30%) and glutathione reductase (8%) have been observed after 25 days of storage in citrate–phosphate–dextrose–adenine storage solution [68]. A similar study reported ∼ 30% depletion of glutathione reductase and glutathione peroxidase after 35 days of preservation in adenine–dextrose–sodium chloride–mannitol storage solution (ADSOL) [75].…”

Section: Factors Promoting Hb Oxidation During Ex Vivo Storage Of Rbcsmentioning

confidence: 99%

“…(B) Hb–membrane adducts can contribute to the release of Hb‐containing membrane vesicles during hypothermic storage [70]. (C) Increased oxidative injury is also the consequence of compromised antioxidant activity (glutathione, catalase) [66,72,73,75], and the presence of molecular oxygen that is available for redox reactions [67,68]. (D) Interactions between the red blood cells and components of the storage container can promote oxidative injury, as in the case of poly(vinyl chloride) bags plasticized with di‐[2‐ethyl hexyl] phthalate (DEHP) [69].…”

Section: Factors Promoting Hb Oxidation During Ex Vivo Storage Of Rbcsmentioning

confidence: 99%

Biopreservation of red blood cells – the struggle with hemoglobin oxidation

2010

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One of the least recognized causes of cellular damage during ex vivo preservation of red blood cells is oxidative injury to the hemoglobin. The latter has been associated with hemolysis through the release of toxic substances and oxidation of vital cell components. This review delineates some of the major pathways that link hemoglobin oxidation and cellular damage, and summarizes the incidence of red blood cell oxidative injury during hypothermic storage, cryopreservation and desiccation stress. Red blood cell hypothermic storage, despite its success, is not exempt from oxidative injury. Growing evidence portrays a time‐dependant oxidative assault including formation of reactive oxygen species, attachment of denatured hemoglobin to membrane phospholipids and the release of hemoglobin‐containing membrane microvesicles throughout storage. Similar symptoms have been observed in attempts to stabilize red blood cells in the dried state, in which methemoglobin levels of reconstituted red blood cells reached 50%. Factors affecting the rate of hemoglobin oxidation during red blood cell ex vivo storage include compromised antioxidant activity, high concentrations of glucose in the storage media and the presence of molecular oxygen. Hemoglobin oxidation largely dictates our ability to effectively preserve red blood cells. Understanding its origins along with investigating methods to minimize it can significantly improve the quality of our future blood products.

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“…It is established that adenylate cyclase activity, Ca 2+ -dependent, adenosine triphosphatase and phosphorylase changes in various conditions and during diseases (hereditary spherocytosis, sickle cell disease, progressive muscular dystrophy). The same is also detected in the case of strenuous exercise [27,28]. Such disorders are caused by low erythrocyte deformability in a sportsman's body [29].…”

mentioning

confidence: 65%

Oxidative stress as a factor in the deterioration of oxygen transfer during exercise

Gunina¹,

Rybina²,

Ataman³

et al. 2021

Fiziol Zh

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Blood oxygen transport regulation by physical activity increase within training dynamics is provided with different mechanisms: from the quantitative and qualitative erythron restructure (including endogenous erythropoietin rise and main erythrocyte index shifts) to change in haemoglobin affinity to oxygen, its heterogeneous structure and blood flow growth as a result of endothelium hyperpolarisation. However, the erythrocyte itself remains a key performer in blood velocity control, due to its structure and functions. This review summarizes the data of modern scientific literature on the characteristics of erythrocytes, which make these cells one of the key links in the oxygen transport system of the blood. The focus on this property of erythrocytes during physical activity is based on the fact that the athlete’s muscles must be supplied with enough oxygen to ensure high performance. Specific training and extra-training factors affecting the content of erythrocytes have been determined. The membrane structure is treated as a significant erythrocyte part in determining its deformation and microvascular blood transport. Enzymes associated with the erythrocyte membrane and affecting cell viability and performance are described. Besides, it is stressed on monitoring erythrocyte indices via modern equipment and assessing lipid peroxidation, which leads to disorders in erythrocyte membrane structure and functions.

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Enzymes and membrane proteins of ADSOL-preserved red blood cells

Cited by 5 publications

References 16 publications

Beneficial Effects of Melatonin on Oxidative Damage Observed During Whole Blood Storage.

Beneficial Effects of Melatonin on Oxidative Damage Observed During Whole Blood Storage.

Biopreservation of red blood cells – the struggle with hemoglobin oxidation

Oxidative stress as a factor in the deterioration of oxygen transfer during exercise

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