Beneficial Effects of Melatonin on Oxidative Damage Observed During Whole Blood Storage.

Abstract

It is well known that oxidative stress rises during the storing period of whole blood and there has been a great interest to improve the quality of stored blood for longer periods. In the present study, we determine the effects of melatonin as an antioxidant on the whole blood indices stored under blood bank conditions. Human blood was collected by venipuncture into citrate-dextrose-phosphate (CDP) bags from healthy volunteers. Immediately after collection, blood was subdivided into two parts and exposed to either melatonin or control saline solutions. Several biochemical parameters were measured on the day of collection and at weekly intervals up to 3 weeks. Mean corpuscular and platelet volumes tended to increase during storing process and with melatonin, these indices remained near to physiologic levels. As expected, acidity, oxidative damage and osmotic fragility increased in stored blood. Interestingly addition of melatonin reduced acidity, oxidative damage by lowering malondialdehyde levels and by increasing superoxide dismutase activity, and osmotic fragility during storage without adversely affecting other biochemical parameters. pH was 6.68 ±0.04 in day 14 of control group while it was 7.00 ± 0.03 in melatonin group. Malondialdehyde level was 1.223±0.05 in day 21 of control group while it was 0.723±0.04 in melatonin group (p< 0.05).Superoxide dismutase activity was significantly higher in melatonin group at days 14 and 21 (151.3±12.2 and 82.8±9.2) compared to saline (111.8±6.5 and 44.8±3.0) (p< 0.05). Through these results, we could confirm a central role for oxidative stress in the mechanism of the most evident blood storage lesions and melatonin seems to exhibit beneficial effects on these lesions. Keywords: Whole blood storage, Oxidative stress, Melatonin ÖZET Kanın Depolanmasında Görülen Oksidatif Hasarda Melatoninin Koruyucu EtkileriKanın depolanması sırasında oksidatif stresin arttığı iyi bilinmekte ve depo kanın kalitesinin daha uzun sürelerle korunması için yoğun çalışmalar yapılmaktadır. Bu çalışmada, kan bankası şartlarında saklanan tam kan örneklerinde bir antioksidan olan melatoninin etkisi araştırılmıştır. Sağlıklı gönüllü bir grup insandan venöz kan örnekleri, antikoagulanlı torbalara alınmıştır. Bağıştan hemen sonra kan, kontrol grubu ve melatonin grubu olarak iki gruba ayrılmış ve bağış gününde ve sonraki üç hafta boyunca her hafta çeşitli biyokimyasal analizler yapılmıştır. Ortalama eritrosit ve trombosit hacim değerleri saklama boyunca artmış, ancak melatoninle bu değerler normale yakın seyretmiştir. Beklendiği gibi, saklanan kanda asidite, ozmotik frajilite ve oksidatif hasar artmıştır. İlginç olarak, melatonin ilavesi asiditeyi, ozmotik frajiliteyi ve oksidatif hasarı (malondialdehit seviyelerini azaltıp superoksit dismutaz aktivitesini artırarak) anlamlı olarak düşürmüştür.Kontrol grubunda 14. gün pH değeri 6.68±0.04 iken, melatonin grubunda bu değer 7.00 ± 0.03 idi. Malondialdehit düzeyi kontrol grubunda

Melatonin is a potential therapeutic molecule for oxidative stress induced red blood cell (RBC) injury : A review

“…Clinically, melatonin has been applied to athletes in maintaining their antioxidant level during preparatory period of competitions (285) with increased activities of catalase and GPx in their RBCs (286). Melatonin is also applied in RBC storage to eliminate the detrimental effects of oxidative damages (287). The protective effect of melatonin on RBC oxidative stress has been observed in senescence accelerated mice (288).…”