The effect of chronic nitric oxide inhibition on vascular reactivity and blood pressure in pregnant rats

Takiuti, Nilton Hideto; Carvalho, Maria Helena Cetelli; Kahhale, Soubhi; Nigro, Dorothy; Barbeiro, Hermes Vieira; Zugaib, Marcelo

doi:10.1590/s1516-31801999000500004

Cited by 13 publications

(5 citation statements)

References 30 publications

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“…Using this experimental model, several studies document effects such as increasing vascular response in vivo (Buhimschi et al, 1995) and in vitro (Khalil et al, 1998;Kublickiene et al, 2000), decreasing or blocking the relaxation of the vascular endothelium (Maeso et al, 1999;Takiuti et al, 1999), and alteration in the remodeling of the uterine arteries (Osol et al, 2009). …”

Section: Discussionmentioning

confidence: 99%

“…After confirmation of pregnancy each rat was weighed and placed in an individual metabolic cage. Pregnant Wistar rats were treated or not, from days 10 to 20 of pregnancy, with L-NAME (70-80 mg/kg/day) in drinking water (Takiuti et al, 1999;Moura et al, 2007). Before starting L-NAME treatment, the mean volume of liquid ingested by the pregnant rats in each cage was determined by measuring the volume of water that the rats had drunk.…”

Section: Experimental Designmentioning

confidence: 99%

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Hepatoprotective and anti-inflammatory effects of silibinin on experimental preeclampsia induced by l-NAME in rats

et al. 2012

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Section: Discussionmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

Hepatoprotective and anti-inflammatory effects of silibinin on experimental preeclampsia induced by l-NAME in rats

et al. 2012

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“…Although L-NAME is commonly directly applied to vessels in ex vivo studies (used for over 20 yr ( 76 )), few studies have explored the effect of administering L-NAME in vivo on vascular reactivity. One study showed that L-NAME administration impaired acetylcholine induced vasorelaxation in rat aortic rings ( 77 ) and another that L-NAME impaired myogenic tone of rat uterine radial arteries ( 78 ). However, it is understood that vascular adaptations in pregnancy are distinct between rats and mice ( 79 ), which may explain the discrepancy.…”

Section: Discussionmentioning

confidence: 99%

The L-NAME mouse model of preeclampsia and impact to long-term maternal cardiovascular health

et al. 2022

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Preeclampsia affects ∼2–8% of pregnancies worldwide. It is associated with increased long-term maternal cardiovascular disease risk. This study assesses the effect of the vasoconstrictor N(ω)-nitro-L-arginine methyl ester (L-NAME) in modelling preeclampsia in mice, and its long-term effects on maternal cardiovascular health. In this study, we found that L-NAME administration mimicked key characteristics of preeclampsia, including elevated blood pressure, impaired fetal and placental growth, and increased circulating endothelin-1 (vasoconstrictor), soluble fms-like tyrosine kinase-1 (anti-angiogenic factor), and C-reactive protein (inflammatory marker). Post-delivery, mice that received L-NAME in pregnancy recovered, with no discernible changes in measured cardiovascular indices at 1-, 2-, and 4-wk post-delivery, compared with matched controls. At 10-wk post-delivery, arteries collected from the L-NAME mice constricted significantly more to phenylephrine than controls. In addition, these mice had increased kidney Mmp9:Timp1 and heart Tnf mRNA expression, indicating increased inflammation. These findings suggest that though administration of L-NAME in mice certainly models key characteristics of preeclampsia during pregnancy, it does not appear to model the adverse increase in cardiovascular disease risk seen in individuals after preeclampsia.

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“…[13] Such changes could be responsible for the higher OPA values observed in pregnant patients. Regarding the lower IOP values during pregnancy, there seems to exist a resistance to angiotensin LL effect, added to an increased nitric oxide action, [14][15][16] causing peripheral vasodilatation. Moreover, prostacyclin promotes generalized vasodilatation, [13] resulting in lower blood pressure values, reaching its nadir in the second trimester, and returning to normality after delivery.…”

Section: Discussionmentioning

confidence: 99%

Analysis of ocular pulse amplitude values in different pregnancy stages as measured by dynamic contour tonometry

Fernandez¹,

Sousa²,

Doi³

et al. 2018

cleverjournal

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Background: Orbital circulation is influenced by systemic hormonal status. Ocular pulse amplitude (OPA) is a surrogate measurement of choroidal blood flow. We investigated the OPA profile during different stages of pregnancy. Design: Cross-sectional study. Participants: We enrolled 24 pregnant and 25 non-pregnant women (age-matched controls). Methods: Data collected included age, pregnancy period, intraocular pressure (IOP), and central corneal thickness (CCT). Pascal dynamic contour tonometry was used to measure OPA values. The mean of three good quality measurements was used for the analysis. Whenever both eyes were eligible, the right eye was arbitrarily selected. Main Outcome Measures: Differences in OPA values between pregnant women (at each trimester) and non-pregnant controls. Results: Mean age and CCT were similar between pregnant women (27.8 ± 6 years, 547 ± 25 µm) and controls (28.9 ± 3.4 years, 546 ± 28 µm; P > 0.25). Pregnant women (mean gestation period, 20.4 ± 9 weeks) had a lower mean IOP than controls (11.4 ± 2.4 vs. 13 ± 2.1 mmHg; P = 0.02). Analysis of covariance (adjusting for IOP difference) revealed that OPA values in women in the 1 st (3 ± 0.6 mmHg) and 2 nd trimesters (2.5 ± 0.7 mmHg) of pregnancy were increased compared to those in the last trimester (1.8 ± 0.6 mmHg) and controls (2.1 ± 0.7; P < 0.05). Multivariate analysis showed that gestation period was the only variable associated with OPA values during pregnancy (r 2 = 0.30, P < 0.01). Age, CCT, and IOP were not statistically significant in this model (P > 0.5). Conclusions: Our results suggest that OPA values are increased in the first two trimesters of pregnancy, returning to normal in the last 3 months. These changes in OPA values seem not be influenced by age, CCT, or IOP.

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The effect of chronic nitric oxide inhibition on vascular reactivity and blood pressure in pregnant rats

Abstract: Acetylcholine-induced vasorelaxation sensitivity was greater in pregnant rats and that blood pressure increased after L-NAME administration while the acetylcholine-induced vasorelaxation response was blocked.

Cited by 13 publications

References 30 publications

Hepatoprotective and anti-inflammatory effects of silibinin on experimental preeclampsia induced by l-NAME in rats

Hepatoprotective and anti-inflammatory effects of silibinin on experimental preeclampsia induced by l-NAME in rats

The L-NAME mouse model of preeclampsia and impact to long-term maternal cardiovascular health

Analysis of ocular pulse amplitude values in different pregnancy stages as measured by dynamic contour tonometry

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