Detection of fetal fibronectin in twin pregnancies in relation to gestational age

Oliveira, Talita Cristina de; Carvalho, Carla Muniz Pinto de; Souza, Eduardo de; Mariani-Neto, C; Camano, Luiz

doi:10.1590/s1516-31801999000300005

Cited by 9 publications

(8 citation statements)

References 13 publications

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“…Such cytoskeleton rearrangement and defects in endothelial cell signals transduction and function may be contributing factors to the characteristic disorganization of myofiber in placentas, where tissue structural capacity for stretch was altered, leading to sPTL-IM. This cause-and-effect interpretation is consistent with the higher incidence of PTL during multiple versus single gestations [36].…”

Section: Structural or Cytoskeletal Componentssupporting

confidence: 80%

Spontaneous Unexplained Preterm Labor with Intact Membrane: Finding Protein Biomarkers through Placenta Proteome

Tan¹,

Daim²,

Jamil³

et al. 2018

Electrophoresis - Life Sciences Practical Applications

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Spontaneous unexplained preterm labor with intact membrane (sPTL-IM) remains as an unresolved challenge in obstetrics due to the complex syndromes involved during preterm birth. Two dimensional-gel electrophoresis (2D-GE) coupled with matrixassisted laser desorption/ionization-time of flight/time of flight (MALDI TOF/TOF) mass spectrometry has become an alternative in screening for potential novel protein-based biomarkers and revealing the pathophysiology of sPTL-IM. To achieve this objective, protein extracted from fetal and maternal sides of the placenta obtained from sPTL-IM (n = 5) and the respective control (n = 10) groups were separated and compared using 2D-gel electrophoresis. MALDI-TOF/TOF mass spectrometry was utilized to identify the differentially expressed proteins between both groups, and the molecular functions of these proteins were studied. A total of 12 proteins were significantly differentiated in sPTL-IM over the control. Differentially expressed proteins were identified to have involved in structural/cytoskeletal components, immune responses, fetal and placenta development, and anticoagulation cascade. More proteins were found to be differentially expressed in the fetal side compared to the maternal side of the placenta. This postulates that the influence of sPTL-IM from fetus is greater than that of the mother. Ultimately, these results might lead to further investigations in elucidating the potential of these proteins as biomarkers and/or drug targets.

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Section: Structural or Cytoskeletal Componentssupporting

confidence: 80%

Spontaneous Unexplained Preterm Labor with Intact Membrane: Finding Protein Biomarkers through Placenta Proteome

Tan¹,

Daim²,

Jamil³

et al. 2018

Electrophoresis - Life Sciences Practical Applications

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“…2,[4][5][6][7][8] Nonetheless, all such predictive diagnostic measures have had mixed results in practice, typically suffering from low sensitivity and selectivity, with low positive predictive value, and a high frequency of false-positive results. [9][10][11] Evi- dence of intrauterine infection is perhaps the most reliable predictive indicator of preterm labor but accounts for only a fraction of premature deliveries; infection seems to be associated with the earliest PL events and commonly with preterm premature rupture of membranes (PPROM). [12][13][14] Thus, no single diagnostic tool has been demonstrated to uniformly, reliably, or consistently predict PL, and vigilant obstetric management of all patients deemed at risk continues, in the absence of definitive diagnoses.…”

Section: Introductionmentioning

confidence: 99%

An Initial Proteomic Analysis of Human Preterm Labor: Placental Membranes

et al. 2006

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Human preterm labor (PL) is the single most significant problem in modern Obstetrics and Gynecology, affecting approximately 10% of pregnancies worldwide, constituting the leading cause of perinatal mortality and morbidity, and contributing significantly to chronic childhood disease. Currently, our molecular understanding of PL remains staggeringly inadequate to reliably diagnose or rationally intervene in PL events; several molecular alterations have been implicated in PL, but these have proven of limited value as diagnostic/prognostic markers. The majority of PL events remain spontaneous and unpredictable: critical care emergencies. Here, we apply functional proteomics to dissect molecular mechanisms of human PL. Human placental tissue was collected in clearly differentiated cases of preterm and term labor. Highly refined two-dimensional gel electrophoresis (2DE) was used for protein separation, coupled with automated differential gel image analysis to compare the resulting proteomic maps. For this initial study, only the most important protein differences were selected for further analysis, that is, proteins that were unique to one sample, and absent from the other, with 100% reproducibility across the sample population. In total, 11 such proteins were identified by tandem mass spectrometry, falling into three distinct functional classes: structural/cytoskeletal components, ER lumenal proteins with enzymatic or chaperone functions, and proteins with anticoagulant properties. These expression changes form the groundwork for further molecular investigation of this devastating medical condition. This approach therefore holds the potential not only to define the underlying molecular components, but also to identify novel diagnostic tools and targets for rational drug intervention.

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“…Em relação ao peso dos RN, observou-se que nos filhos das diabéticas, este foi em média 360 g superior ao dos controles, e a incidência de recémnascidos com peso superior a 4.000 g foi três a cinco vezes maior do que a observada em quaisquer dos controles. O peso mais elevado dos RN de mães diabéticas foi semelhante àquele relatado por Oliveira et al 11 . A incidência de 17% de RN com peso superior a 4.000 g foi maior do que a de 11,5% descrita por Kitzmiller et al 3 .…”

Section: Discussionunclassified

Via de Parto e Resultados Perinatais em Gestantes Diabéticas

Pereira¹,

Faúndes²,

Parpinelli³

et al. 1999

Rev. Bras. Ginecol. Obstet.

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Objetivo: apresentar os resultados perinatais obtidos a partir da aplicação de um protocolo de assistência às gestantes diabéticas no Centro de Atenção Integral à Saúde da Mulher (CAISM) da UNICAMP. Métodos: foram estudadas 90 gestantes diabéticas que iniciaram controle pré-natal na instituição e foram submetidas a este protocolo. Foram comparadas com dois grupos controles de 180 gestantes cada: um constituído por gestantes pareadas por idade e número de gestações (controle A) e outro por gestantes aleatoriamente selecionadas (controle B). Nos três grupos foram avaliadas as seguintes variáveis: tipo de parto, indicações de cesárea, idade gestacional, índice de Apgar ao primeiro e quinto minuto de vida, peso e adequação de peso para idade gestacional, morbidade e mortalidade perinatal. Para a análise estatística utilizaram-se médias, desvio-padrão, os testes t de Student e do χ2. Resultados: entre as gestantes diabéticas ocorreu maior incidência de cesáreas, recémnascidos prematuros e grandes para a idade gestacional (GIG), assim como uma maior freqüência de patologias neonatais (hipoglicemia, hipocalcemia, hiperbilirrubinemia, desconforto respiratório e depressão neonatal). A incidência de Apgar <7 e a mortalidade perinatal foram significativamente maiores do que no grupo controle aleatoriamente selecionado, mas a diferença desapareceu quando se comparou ao grupo controle pareado por idade e número de gestações. Conclusões: apesar de o protocolo visar um perfeito controle metabólico nas gestantes diabéticas, os resultados perinatais ainda são desfavoráveis em comparação às gestantes não-diabéticas.

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Detection of fetal fibronectin in twin pregnancies in relation to gestational age

Cited by 9 publications

References 13 publications

Spontaneous Unexplained Preterm Labor with Intact Membrane: Finding Protein Biomarkers through Placenta Proteome

Spontaneous Unexplained Preterm Labor with Intact Membrane: Finding Protein Biomarkers through Placenta Proteome

An Initial Proteomic Analysis of Human Preterm Labor: Placental Membranes

Via de Parto e Resultados Perinatais em Gestantes Diabéticas

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