Use of autologous fibrin glue in dermatologic surgery: application of skin graft and second intention healing

Abstract

Objective:To evaluate the efficiency of biological sealant, an autologous fibrin glue, in dermatological surgery. Design: Randomized clinical trial. Setting: The Dermatology Service of Hospital das Clínicas, Universidade de Campinas (UNICAMP), referral center. Patients: 14 patients with malign epithelial cutaneoos tumors participated in the evaluation, each having two tumors, generally facial and symmetrical, in order to perform a comparative evaluation on the same individual. Procedures: The glue was prepared beforehand with a sample of autologous blood. Surgical extirpation of the tumor was followed by grafts or second intention healing. Outcomes: The efficiency of the sealant was then evaluated in relation to hemostasis, adhesion, surgical time and evolution of the granulation tissue, clinically and histologically. Results: Immediate hemostasis and graft adhesion, with a significant reduction of surgical time, and in the open wounds there was immediate hemostasis and a clinical increase in granulation tissue, but with no histological differences among the groups on the 7 th day. Conclusion: It is an adjuvant resource in skin cancer surgery.Uniterms: Fibrin glue. Wound healing. Skin cancer. Dermatologic surgery.

Experimental use of fibrin glue derived from snake venom in non-pregnant canine uterus

“…These results are similar to those seen in rat colon anastomosis, where the same glue showed a significant reduction in surgical time, immediate hemostais, and a higher quantity of granulation tissue (5). The utilization of commercial autologous fibrin glue as suture reinforcement in human malignant skin tumors (7) has also shown good results.…”

“…The hemostatic effect of autologous fibrin glue derived from snake venom and the increase in granulation tissue were demonstrated in skin grafts when compared to second intention healing, although the histological alterations were not different (7).…”

Tissue therapy with autologous dermal and epidermal culture cells for diabetic foot ulcers

“…The autologous fibrin glue was prepared according to the technique described by de Moraes et al (1998); venous blood was collected from each patient and placed in individual tubes containing 3.8% of sodium citrate (9:1). The plasma was obtained after centrifugation for 10 min at 3,000 rpm.…”

10 Autologous Hemotherapy

“…Various working groups have reported on the preparation and use of autologous fibrin glue in surgery [13,44,49]. Standard methods have not yet been established [46].…”