Variations in white blood count, thromboxane B2 levels and hematocrit in chronic venous hypertension

Abstract

Objective: To analyze variations in leukocyte count and thromboxane B 2 production in the femoral vein of patients with chronic venous hypertension (CVH). Design: Prospective clinical study, controlled, non randomized and open. Location: Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, referral center, university hospital. Participants: 15 patients with recurring stasis ulcer were analyzed, selected randomly from the venous diseases outpatient center, and 4 without lower limb venous alterations were also analyzed. Intervention: Blood samples from the femoral and brachial veins were drawn following supine and 45º reverse Trendelenburg. Main outcomes measures: Direct leukocyte count and analysis of the thomboxane B 2 with enzyme linked immunosorbent assay test. Results: After 30 minutes in reverse Trendelenburg, patients with CVH showed a leukocyte count reduced by ±27% (p=0.02) and thromboxane B 2 levels increased by ±158% (p=0.02). Conclusions: We suggest that future studies of medications for stasis ulcers include their effects on leukocyte entrapment and thromboxane B 2 production in the lower limb venous system.

Influence of antioxidant complex on the adhesion of leukocytes in chronic venous insufficiency of lower limbs in rats

“…As several authors have noted, even in the conditions of short-term venous hypertension, the leukocytes delay in the vessels of the lower limbs and the increase in ICAM-1 content in patients’ plasma with CVI of lower limbs are observed. [2728] Similar changes were observed by Bergan, et al ., but on the model of arteriovenous anastomosis and occlusion of the mesenteric venule in rats. They observed accumulation of leukocytes in the valvate structures and vein walls and the increased ICAM-1 expression of vascular endothelium in the conditions of raised venous pressure.…”

Leg Ulcers: Biochemical Factors

“…It has been shown that leucocytes retrieved from the lower limbs of volunteers and patients with venous disease following venous hypertension undergo activation [I1, 12]. One study has reported the results of venous hypertension on the leucocyte count in the femoral vein [13]. In this paper, patients with chronic venous disease initially lay supine, following which blood samples were taken from the femoral and arm veins.…”