How should PCNA be assessed? Total of stained cells or only the most intensely stained ones?

Kemp, Carla; Alberti, Vânia Nosé; Lima, Geraldo Rodrigues de; Carvalho, Filomena Marino

doi:10.1590/s1516-31801998000200005

Cited by 7 publications

(8 citation statements)

References 17 publications

(73 reference statements)

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“…In this report, we considered the total PCNA positive cells regardless of the staining intensity. Kemp et al (1998) demonstrated that the total of stained cells was more representative of tumor aggressiveness and were better correlated with the anatomopathological parameters in breast cancer than the intensity of staining.…”

Section: Discussionmentioning

confidence: 99%

Identification of the AgNORs, PCNA and ck16 proteins in oral lichen planus lesions

Fonseca

Carmo

2001

Oral Diseases

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The expression of proliferating cell nuclear antigen (PCNA), cytokeratin 16 (ck16) and Ag nucleolar organizer regions (AgNORs) were assessed in 20 cases of lichen planus, 20 cases of keratosis and 20 cases of normal oral mucosa in order to evaluate the rate of keratinocyte proliferation in these tissues. Three hundred cells were counted in each sample: 100 basal cells, 100 suprabasal cells and 100 squamous cells. The mean number of AgNORs and the percentage of PCNA positive cells were calculated. Except from similar staining of suprabasal cells of lichen planus and keratosis, PCNA and AgNORs values were higher in all layers of lichen planus than in both keratosis and normal oral mucosa. The three groups showed similar ck16 immunostaining: all of the cells were positive, except those of the basal layer. The results suggest that the keratinocyte proliferation index is higher in lichen planus than in keratosis and normal mucosa. Besides, ck16 should not be used to differentiate the entities studied.

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Section: Discussionmentioning

confidence: 99%

Identification of the AgNORs, PCNA and ck16 proteins in oral lichen planus lesions

Fonseca

Carmo

2001

Oral Diseases

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“…No epitélio mamário de tecido adjacente ao fibroadenoma de mulheres no menacme, o tamoxifeno reduziu a proliferação do lóbulo medido pelo índice mitótico, volume nuclear, número de lisossomos, PCNA e MIB-1 14,17,22 . Estes trabalhos demonstraram também que o tamoxifeno atua, de forma antagônica ao estrogênio, no epitélio da mama normal de mulheres no menacme.…”

Section: Discussionunclassified

“…A contagem de células foi feita por meio de objetiva de 40 vezes, com aumento final de 400 vezes, contando-se, no mínimo, 500 células a partir da área com maior reação do Ki-67, com aleatorização dos campos posteriores. Considerou-se positiva a célula cujo núcleo exibisse a coloração castanha, independentemente da disposição e intensidade 22 .…”

Section: Pacientes E Métodosunclassified

Efeitos de diferentes doses de tamoxifeno sobre a proliferação celular do epitélio mamário

Facina¹,

Baracat²,

Lima³

et al. 2003

Rev. Bras. Ginecol. Obstet.

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“…PCNA (proliferating cell nuclear antigen) is a subunit of DNA polymerase and cell cycle-dependent protein whose maximum appearance in the S phase. Some research shows that the expression of PCNA is connection with the low grade tissues[ 35 , 36 ]. Wang JJ found that the gene expression of nuclear PCNA increased obviously, and the labeling index of PCNA was 15.…”

Section: Reviewmentioning

confidence: 99%

Chinese herb related molecules of cancer-cell-apoptosis: a minireview of progress between Kanglaite injection and related genes

Dong

2008

J Exp Clin Cancer Res

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Many kinds of Chinese herb had been confirmed to have the character of anti-tumor, clinical reports about anti-tumor effects of Chinese herb had also been found in recent years, but most of the reports were focused on the clinical treatment of effectiveness for Chinese herb, on the other hand, review about Chinese herbal related with molecules on cancer-cell-apoptosis was seldom, many scientists could not believe such kinds of clinical describes about anti-tumor effects for Chinese herb, because these describes were lack of molecular biology evidence. Kanglaite(KLT) injection is an anti-tumor new drug which extracts from Chinese medicine-coix seed with modern advanced pharmaceutical technology, it is also a new biphase extended-spectrum anticancer medicine, the food and drug administration(FDA) of United States also approved a phase II trial of KLT to test its efficacy in treating non-small-cell lung cancer. Some studies show it could inhibit some anti-apoptotic gene and activate some pro-apoptotic gene, its injection solution is one of the new anticancer medicine that can significantly inhibit a various kinds of tumor cells, so it has become the core of research that how to further explore KLT injection to promote tumor cell apoptosis by impacting on related genes. In this review, the relationship between KLT and some tumor cell apoptosis molecules had been discussed and reviewed generally. ReviewIn recent years, with the lucubrate on tumor cell biology and molecular biology, it has been recognized that the occurrence and development of tumor is not only the result of cell proliferation disorders and disdifferentiation, but also closely correlated with the abnormal apoptosis [1,2]. Although abnormal apoptosis can promote the occurrence and development of tumor, we can also treat tumor by promoting apoptosis of cancer cell [3,4]. Thus it has become the new target in oncotherapy by way of inducing apoptosis of cancer cell. Kanglaite(KLT) injectionis a diphasic broad-spectrum anti-tumor new drug which has depressant effect on many kinds of tumor cells, it is extracted from the chinese crude drug-coixenolide [5,6] and made use of the latest and most complex modern high technologies in process of preparation [7]. Animal experiments show that KLT mainly block G2+M phase of cell circle, thereby reducing the mitotic division

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How should PCNA be assessed? Total of stained cells or only the most intensely stained ones?

Abstract: Higher nuclear (NG3) and histological (HGIII) grading, associated with a high PCNA index (> 50), distinguish high-risk patients, and it is more appropriate considering all the stained cells as representative of PCNA indices, thus reflecting tumor aggressiveness.

Cited by 7 publications

References 17 publications

Identification of the AgNORs, PCNA and ck16 proteins in oral lichen planus lesions

Identification of the AgNORs, PCNA and ck16 proteins in oral lichen planus lesions

Efeitos de diferentes doses de tamoxifeno sobre a proliferação celular do epitélio mamário

Chinese herb related molecules of cancer-cell-apoptosis: a minireview of progress between Kanglaite injection and related genes

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