Fabrication and characterization of nanofibrous scaffold developed by electrospinning

Abstract

Electrospinning has been recognized as an efficient technique for the forming of polymer nanofibers. Silk fibroin (SF) nanofibers were electrospun from SF solution using trifluoroacetic acid solution as a solvent. In the present work, we have systematically evaluated the effects of instrument parameters, including applied voltage, tip-target distance, solution flow rate, solution parameters; such as polymer concentration and solution viscosity on the morphology of electrospun SF fibers. The applied voltage and flow rate was monitored at fixed tip target distance during the electrospinning process and it was correlated with the characteristics of the fibers obtained. The number of deposited fibers also increases with the applied voltage. Also, viscosity, flow rate and applied voltage strongly affect the shape and morphology of the fibers. A particular interest, we demonstrated that by monitoring the applied voltage and flow rate it is possible to control the fibers morphology and bead concentration. Rheological study showed a strong dependence of spinnability and fiber morphology on solution viscosity. Solution concentrations has been found to most strongly affect fiber size, with fiber diameter increasing with increasing solution concentration and the morphology of the deposition on the collector changed from spherical beads to interconnected fibrous networks. FTIR analysis clearly shows that there are no spectral differences between fibers and which suggests that there was no chemical modification developed during the process. Under optimized conditions, homogenous (not interconnected) SF fibers with a mean diameter of 234 nm were prepared.

Polysorbate Enhanced Progesterone-Loaded Drug Diffusion from Macromolecular Fibrous Patches for Applications in Obstetrics and Gynaecology

“…However, at higher shear rates, the molecules align with the spindle, reducing resistance and providing a lower viscosity. The decrease in the viscosity as shear rate increases is known due to the entangled chain networks in the polymer solution, and with higher viscosities a more homogenous spread of solvent molecules over the entangled polymer occurs [39].…”

Investigation of the effects of environmentally friendly degumming methods on silk dyeing performance

“…62,64,65 Moreover, in the literature there is information that sericin gives peaks at 3200, 1600, and 1500 cm -1 wavelengths, which are wider than those of fibroin. [66][67][68][69][70] From inspection of the transmittance spectra depicted in Figure 5, the broad spectrum from 3600 to 3100 cm À1 and the peak at around 3275 cm À1 are representative of the N À H stretching band that is built on the O À H stretching band in the silk. The peak at around 1610 cm À1 probably represents C ¼ O stretching (amide I), while the secondary amides at approximately 1510 cm À1 (N-H bending) and the tertiary amides in the region of 1225 cm À1 are due to the stretch of the bond C-N. 66,[71][72][73][74][75][76][77] According to Figure 5, no major difference was observed in the IR spectra of the degummed silk fibers.…”

Revisión: Fibroína de seda y sus potenciales aplicaciones en empaques biodegradables para alimentos/Review: silk fibroin and their potential applications on biodegradable food packaging

“…La FS es un polímero natural que presenta una serie de características de gran interés para el desarrollo de nuevos materiales, entre las que se destacan sus propiedades mecánicas y su biodegradabilidad [48,49]. Estas características hacen que la FS pueda tener potencial aplicación como biomaterial de empaques.…”