Natural rubber latex used as drug delivery system in guided bone regeneration (GBR)

Herculano, Rondinelli Donizetti; Silva, Cecília Pereira; Ereno, Cibele; Guimarães, Sérgio Augusto Catanzaro; Kinoshita, Ângela; Graeff, Carlos Frederico de Oliveira

doi:10.1590/s1516-14392009000200023

Cited by 104 publications

(76 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One candidate already described in literature [7][8][9][10][11] as a potential carrier is the natural rubber latex (NRL) from Hevea brasiliensis. The NRL membrane is an important inductor of the healing process of wounds, being used in several medical applications like prosthetics and bone grafts [12][13][14][15][16] .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of sodium diclofenac release using natural rubber latex as carrier

et al. 2014

View full text Add to dashboard Cite

Sodium Diclofenac is a non-steroidal anti-inflammatory drug (NSAID) taken to reduce inflammation and, as an analgesic, reduce pain. Although this drug is widely used in the general population, properties such as the short half-time and some side effects restrict its clinical use. The most common side effects are: gastric irritation, gastritis, peptic ulcer and bleeding. Studies involving biomaterials as carrier for drug release have been proving their efficiency in overcoming those problems and better controling the release rate and targeting of the drug. Natural rubber latex (NRL) has been proven excellent for its biocompatibility and ability to stimulate angiogenesis, cellular adhesion and the formation of extracellular matrix, promoting the replacement and regeneration of tissue. In this work, a NRL membrane is used to deliver sodium diclofenac. Sodium diclofenac (NaDic) was found to be adsorbed on the NRL membrane, with little or no incorporation into the membrane bulk, according to energy dispersive Scanning Electron Microscopy with X-Ray microanalysis (SEM-EDS) spectroscopy. In addition, FT-IR shows that there is no molecular-level interaction between drug and NRL. Already, the X-Ray Diffraction (XRD) of NaDic-NRL shows a broader one spectrum than the sharper halo (amorphous characteristic XRD spectrum) of pure NRL. More importantly, the release time of diclofenac in a NRL membrane in vitro was increased from the typical 2-3 h for oral tablets to ca. 74 h. The kinetics of the drug release could be fitted with a double exponential function, with two characteristic times of 0.899 and 32.102 h. In this study, we demonstrated that the interesting properties provided by NRL membranes combined with a controlled release of drug is relevant for biomedical applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of sodium diclofenac release using natural rubber latex as carrier

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Currently, more than 90% of NRL comes from one single tropical tree species Hevea brasiliensis (para rubber tree). Recently, promising results in the use of NRL from Hevea brasiliensis rubber tree to produce replacement and regeneration tissues has been reported [1][2][3] . Among them, wound healing in cutaneous tissues, eardrum replacement, bone regeneration, and dental alveolus replacement afford a unique means to repair or replace the failing organs or tissues 2) .…”

Section: Introductionmentioning

confidence: 99%

Evaluation on Cytotoxicity of Natural Rubber Latex Nanoparticles and Application in Bone Tissue Engineering

et al. 2017

View full text Add to dashboard Cite

To broaden the knowledge of cytotoxicity of natural rubber latex (NRL) nanoparticles we for the first time examined the latex biocompatibility in vitro against mouse calvaria preosteoblastic cells (MC3T3-E1) and human alveolar basal epithelial (A549) cells. For NRL nanoparticles, the half maximal inhibitory concentration (IC 50 ) value for MC3T3-E1 cells is one order of magnitude higher in toxicity as compared to that of A549 cells (3.99 mg/mL for MC3T3-E1 and 0.33 mg/mL for A549 cells). Owing to fractionation of NRL nanoparticles by ultra-centrifuge, the effect of the non-rubber constituents on the cytotoxicity was clarified. The suppression on the proliferation for A549 cells incubated with NRL nanoparticles was demonstrated by the cell cycle distribution. The in vitro study on osteogenic differentiation and expressions of proteins and characteristic genes of MC3T3-E1 cells demonstrated the promising results of the NRL nanoparticles for application in bone tissue engineering.

show abstract

“…Latex membranes have been used to promote the activity of bone regeneration through the Guided Bone Regeneration (GBR) model system by incorporating bone morphogenetic proteins on NR membranes acting as protein delivery system [25][26][27][28]. In this context, it is possible to combine the NR characteristics as a physical barrier (to the epithelial cells or other components) with particles of specific functionalities, such as Ca/P, to produce a functionalized bandage.…”

Section: Introductionmentioning

confidence: 99%