Synthesis and characterization of poly (vinyl alcohol) hydrogels and hybrids for rMPB70 protein adsorption

Abstract

Polyvinyl alcohol (PVA), PVA crosslinked with glutaraldehyde hydrogels (PVA/GA), PVA with tetraethylorthosilicate (PVA/TEOS) and PVA/GA/TEOS hybrids with recombinant MPB70 protein (rMPB70) incorporated were chemically characterized by Fourier transform infrared spectroscopy (FTIR). FTIR spectra of PVA hydrogel samples showed the absorption regions of the specific chemical groups associated with poly(vinyl alcohol) (-OH, -CO, -CH2) and PVA/GA confirming the formation of crosslinked hydrogel (duplet -CH). It was observed C-H broad alkyl stretching band (n = 2850-3000 cm-1) and typical strong hydroxyl bands for free alcohol (nonbonded -OH stretching band at n = 3600-3650 cm-1), and hydrogen bonded band (n = 3200-3570 cm-1). The most important vibration bands related to silane alcoxides have been verified on FTIR spectra of PVA/TEOS and PVA/GA/TEOS hybrids (Si-O-Si, n = 1080 and n = 450 cm-1; Si-OH, n = 950 cm-1). FTIR spectra of f PVA hydrogel with rMPB70 incorporated have indicated the specific groups usually found in protein structures, such as amides I, II and III, at 1680-1620 cm-1, 1580-1480 cm-1 and 1246 cm-1, respectively. These results have given strong evidence that recombinant protein rMPB70 was successfully adsorbed in the hydrogels and hybrids networks. These PVA based hydrogels and hybrids were further used in immunological assays (Enzyme-Linked Immunosorbent Assay - ELISA). Tests were performed to detect antibodies against rMPB70 protein in serum samples from bovines that were positive in the tuberculin test. Corresponding tests were carried out without PVA samples in microtiter plates as control. Similar results were found for commercially available microplates and PVA based hydrogels and hybrids developed in the present work regarding to immunoassay sensitivity and specificity response

Evaluation of the solubility of the HPMC: PVA blends in biological fluids in vitro

“…The region of 1120-1020 cm -1 (ν C-O-C ) presented coincident bands for both polymers 12 . However HPMC and PVA and their blends can be differentiated by a sharp carbonyl band in 1700 cm -1 which is observed only when PVA is presented.…”

Sol–gel derived poly(vinyl alcohol)/maleic acid/silica hybrid membrane for desalination by pervaporation

“…FTIR spectra confirmed the formation of PVA/MA and PVA/MA/silica hybrid with network crosslinking. The pure PVA sample (Figure 1-a) shows the typical C-H broad alkyl stretching band (2800-3000 cm -1 ) and the hydrogen bonded hydroxyl band (3200-3570 cm -1 ) [18]. The peak at 1000-1100 cm -1 was assigned to the C-O stretching vibration of the secondary alcohol (-CH-OH) of PVA.…”

DESIGNED STRUCTURE AND MAGNETIC CHARACTERISTIC STUDIES OF MAGNETIC IRON OXIDE (Fe3O4) NANOPARTICLES COATED BY POLYVINYL ALCOHOL AND POLYVINYL ALCOHOL-LINKED WITH GLUTARALDEHYDE

“…25 The presence of magnetic Fe-O bending in 667 -420 cm -1 region showed the existence of magnetite Fe3O4 and maghemite γ-Fe2O3, as shown in Fig.-2 (inset). This result was in good agreement with the previous report by Monfared and Parchegani.…”