Effects of extraction methods of phenolic compounds from Xanthium strumarium L. and their antioxidant activity

Abstract

Recebido para publicação em 09/11/2011 Aceito para publicação em 04/06/2013 RESUMO: Foram avaliados os efeitos de métodos de extração e de solventes sobre o rendimento de extrato, conteúdo de fenólicos totais, atividade antioxidante, e composição dos compostos fenólicos de Xanthium strumarium L. A atividade antioxidante foi determinada pelo método do radical 2,2-difenil-1-picrilhidrazila (DPPH), e a composição dos compostos fenólicos foi determinada por cromatografia líquida de alta eficiência e por espectrometria de massas com ionização por electrospray. Todos os resultados foram afetados pelo método de extração e, principalmente pelo solvente utilizado, sendo que os melhores resultados foram obtidos com o solvente metanol. Os extratos metanólico e etanólico apresentaram forte ação antioxidante e os principais compostos fenólicos encontrados nos extratos foram os ácidos ferúlico e clorogênico. Palavras-chave:Xanthium strumarium, carrapicho, atividade antioxidante, compostos fenólicos, IAA ABSTRACT: Efeito de métodos de extração de compostos fenólicos de Xanthium strumarium L. e suas atividades antioxidantes. The effect of extraction methods and solvents on overall yield, total phenolic content, antioxidant activity, and the composition of the phenolic compounds in Xanthium strumarium extracts were studied. The antioxidant activity was determined by using 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), and the composition of the phenolic compounds was determined by HPLC-DAD and LC/MS. All results were affected by the extraction method, especially by the solvent used, and the best results were obtained with the methanol extract. The methanolic and ethanolic extracts exhibited strong antioxidant activity, and the chlorogenic and ferulic acids were the most abundant phenolic compounds in the extracts.

Seasonality modifies rosemary’s composition and biological activity

“…Determination of total phenolic compounds (TPC) was done on the extracts by the classic Folin-Ciocalteu method by Scherer and Godoy (2014). A 0.5 mL aliquot of the extracts at a 0.5 mg/mL concentration in methanol and 2.5 mL of the Folin reagent diluted in distilled water (1/10) were injected into a test tube.…”

In Vivo and in Vitro Antileishmanial Effects of Methanolic Extract From Bark of Bursera Aptera

“…According to the literature, phenols are the main compounds responsible for the antioxidant activity; for this reason, MEBA was analyzed (Scherer and Godoy, 2014). The results showed that MEBA displayed a TPC of 61.2 mg GAE/g.…”

Determination of the isolated Rutin And Quercetin Contents In Syrian Ficus Carica L. Leaves Extracts

“…Studies indicate that rutin has many functional properties such as antioxidant, anti-inflammatory effect in humans (18). However, its antioxidant activity and its bioavailability were lower than quercetin in rats by oral management (19)(20)(21). The disadvantage of quercetin is poorly absorbed in the small intestine, and the bioavailability of it was mentioned to be less than one per cent in the capsule form in human beings (22).…”