Germinação assimbiótica e desenvolvimento de Dendrobium nobile Lindl. sob efeito de reguladores vegetais no tratamento pré-germinativo

Soares, Jackeline Schultz; Rosa, Yara Brito Chaim Jardim; Suzuki, Rogério Mamoru; Scalon, Silvana de Paula Quintão; Rosa, Edgard Jardim

doi:10.1590/s1516-05722012000400007

Cited by 10 publications

(7 citation statements)

References 8 publications

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“…In Cattleya bicolor, Souto et al (2010) also described the positive effects of auxin on root formation, although at high concentrations it hindered that process. Soares (2012) reported that the presence of BA stimulated root formation and root growth in length in Dendrobium nobile Lindl. This author also confirms the fact that increases in root length are beneficial to plants since they can explore larger substrate areas and thus absorb nutrients necessary for their development.…”

Section: Eff Ects Of Culture Media Sucrose and Growth Regulators On mentioning

confidence: 99%

Asymbiotic germination, multiplication and development of Alatiglossum fuscopetalum (Orchidaceae) as affected by culture medium, sucrose and growth regulators

Ferreira¹,

Vasconcelos²,

Silva³

et al. 2017

Iheringia Ser Bot

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-This investigation aimed at studying the germination and initial growth of Alatiglossum fuscopetalum (Hoehne) Baptista under in vitro conditions. The eff ects of Murashige/Skoog (MS and ½MS), Knudson (KN), and Vacin/Went (VW) culture media on the in vitro germination of A. fuscopetalum were analyzed. The infl uence of these media on the multiplication and initial growth of 90-day-old seedlings, and of diff erent sucrose, benzyladenine [BA] and indole-butyric acid [AIB] concentrations on the multiplication and growth of 120-day-old plants were also evaluated. The results revealed that ½MS was the most appropriate medium for germination. Regarding the growth of 90-day-old seedlings, MS provided the best outcome. The most eff ective medium for the multiplication and growth of 120-day-old plants was ½MS. Sucrose ranging from 1% to 3% favored plant growth. BA at 1 mg L -1 and IBA at 2 mg L -1 were the best growth regulator treatments for shoot proliferation and root growth, respectively.

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Section: Eff Ects Of Culture Media Sucrose and Growth Regulators On mentioning

confidence: 99%

Asymbiotic germination, multiplication and development of Alatiglossum fuscopetalum (Orchidaceae) as affected by culture medium, sucrose and growth regulators

Ferreira¹,

Vasconcelos²,

Silva³

et al. 2017

Iheringia Ser Bot

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“…Main root development was not influenced by gibberellic acid at any concentration in any of the experiments (Figure 2A and B). Soares et al (2012) reported a positive influence of GA 3 on the shoot length in the orchid Dendrobium nobile Lindl. They also observed that plant height increased linearly with the increase of GA 3 concentration, suggesting a correlation between the two.…”

Section: Resultsmentioning

confidence: 99%

Seed origin, storage conditions, and gibberellic acid on in vitro germination of Campomanesia adamantium (Cambess.) O. Berg

Damiani¹,

Silva²,

Goelzer³

et al. 2016

Afr. J. Biotechnol.

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Campomanesia adamantium (Cambess.) O. Berg seeds (guavira) are recalcitrant and rapidly lose viability upon removal from the fruit, making difficult the long-term storage. In vitro germination could be used as an important tool to overcome the issues related to this short viability. It might help seed conservation and species propagation. The purpose of this study was to evaluate in vitro germination of guavira seeds collected from different sites and stored under different conditions. Also, the sowing of these seeds in MS medium supplemented with different concentrations of gibberellic acid (GA 3 ) was evaluated. Seeds from the local garden were treated with: 0, 1.0, 2.0, 3.0, or 4.0 mg L -1 GA 3 . Seeds from a local farmer's market were treated with: 0, 2.5, 5.0, 7.5 or 10 mg L -1 GA 3 . We evaluated the germination rate, the average length of the shoot and main root, and the number of leaves. The C. adamantium seeds were sown immediately after harvest and treated with GA 3 , regardless concentration, increased germination rate by at least 10%, whereas 1.0 mg L -1 GA 3 resulted in 100% germination. The shoot length increased linearly with increasing concentration of the growth regulator. Different concentrations of GA 3 had no effect on the development of the main root and leaves. Seeds acquired from a local farmer's market showed lower germination rate than those sown immediately after harvesting, and did not differ in the rate of germination under different treatments with GA 3 . Furthermore, around 25% of those seedlings had abnormal leaf morphology. C. adamantium seeds stored at 4°C and -20°C for 60 days did not germinate successfully, suggesting that seeds under cold storage conditions cannot be used for germplasm purposes.

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“…The use of green pod culture also prevents direct contact of seeds with the chemical disinfectant, which is potentially toxic to tissues (Yeoman andMacleod 1977, George 1993), thus improving the chance (and thus number) of seeds that are able to germinate. Soares et al (2012) disinfected fruits before seed inoculation in vitro, and observed higher percentage germination (80-100%) than when seeds were excised from fruits and then disinfected, obtaining only 46.47% germination (Tab. 1).…”

Section: Choice Of Methods For Surface Disinfectionmentioning

confidence: 98%

Tissue disinfection for preparation of Dendrobium in vitro culture

Silva¹,

Winarto²,

Dobránszki

et al. 2016

Folia Horticulturae

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Establishing an aseptic in vitro culture for Dendrobium, or for any plant in fact, is the most important step towards developing an effective in vitro tissue culture including micropropagation protocol. Success in initial aseptic culture will contribute to the successful production of in vitro cultures that may involve the initiation or formation of callus and/or protocorm-like bodies (PLBs), the induction, regeneration or multiplication of shoots, and the preparation and proliferation of plantlets suitable for acclimatization. The initiation of an aseptic culture is closely related to the appropriate selection of an explant source and its preparation, including its (in vivo) pre-treatment if necessary and subsequent disinfection procedures. Care in the choice of explant and the application of an appropriate disinfection protocol can successfully reduce, or eliminate, contamination in in vitro cultures while reducing the negative impact on plant tissues and plantlet regeneration. Many unique aseptic culture procedures for Dendrobium genus have been reported in the literature, very often specific to particular tissues or genotypes, and this review not only highlights the details of such protocols, but also provides practical advice for novice -and even seasoned -orchidologists who wish to research Dendrobium in vitro, although it is cautioned that there is currently no universal aseptic culture procedure that can be applied to all conditions, all explants or all genotypes.

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Germinação assimbiótica e desenvolvimento de Dendrobium nobile Lindl. sob efeito de reguladores vegetais no tratamento pré-germinativo

Cited by 10 publications

References 8 publications

Asymbiotic germination, multiplication and development of Alatiglossum fuscopetalum (Orchidaceae) as affected by culture medium, sucrose and growth regulators

Asymbiotic germination, multiplication and development of Alatiglossum fuscopetalum (Orchidaceae) as affected by culture medium, sucrose and growth regulators

Seed origin, storage conditions, and gibberellic acid on in vitro germination of Campomanesia adamantium (Cambess.) O. Berg

Tissue disinfection for preparation of Dendrobium in vitro culture

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