Calogênese em Cissus sicyoides L. a partir de segmentos foliares visando à produção de metabólitos in vitro

Rodrigues, Fabíola Rebouças; Almeida, Weliton Antônio Bastos de

doi:10.1590/s1516-05722010000300011

Cited by 2 publications

(2 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A utilização em plantas medicinais pode auxiliar no aumento de metabólitos secundários úteis aos seres vivos de modo que as espécies possam ser mais exploradas economicamente (Rodrigues & Almeida, 2010).…”

unclassified

Multiplicação in vitro de segmentos apicais caulinares de segurelha (Satureja hortensis L.)

Navroski

Waldow

Reiniger

et al. 2014

Rev. bras. plantas med.

View full text Add to dashboard Cite

RESUMO:O presente estudo teve por objetivo avaliar o efeito de BAP, na presença e ausência de ANA, sobre a multiplicação in vitro de segmentos apicais caulinares de Satureja hortensis. Os explantes foram isolados de plântulas germinadas in vitro e cultivados em meio nutritivo MS. O delineamento experimental utilizado foi o inteiramente casualizado, em esquema fatorial 2x5, correspondendo às concentrações de ANA (0 e 1 µM) e de BAP (0; 5; 10; 15 e 20 µM), com seis repetições, cada uma composta por três explantes. Para a porcentagem de explantes com brotações houve efeito significativo para o fator BAP, aumentando à medida que cresce a concentração da citocinina. A variável "número de brotos por explante" apresentou interação entre os fatores, havendo a maior formação de brotos na presença de ANA na faixa de 10 -15 µM de BAP. Na presença da auxina, o maior valor ocorreu com 15 µM de BAP tendendo a diminuir independente da presença de ANA. O enraizamento dos segmentos apicais foi maior na presença de ANA e ausência de BAP, diminuindo com o aumento da citocinina. O número de folhas foi influenciado pela maior concentração de BAP sendo que a 15 µM foi observado o maior número, tendendo à queda com aumento da concentração. A concentração de 15 µM de BAP, independente de ANA, proporcionou os melhores resultados na multiplicação da espécie produzindo aumento de brotações e número de folhas, à exceção do enraizamento que foi influenciado pela auxina.Palavras-chave: cultura de tecidos, regulador de crescimento, plantas medicinais, Lamiaceae. ABSTRACT:In vitro multiplication of apical shoot segments of Summer Savory(Satureja hortensis L.).This study aimed to evaluate the effect of BAP in the presence and absence of NAA, on the in vitro multiplication of shoot apical segments of Satureja hortensis. The explants were isolated from seedlings germinated in vitro and cultured in nutrient medium MS. The experimental design was completely randomized in a 2x5 factorial arrangement, corresponding to the concentrations of NAA (0 and 1 µM) and BAP (0, 5, 10, 15 and 20 µM), with six replicates, each consisting of three explants. For the percentage of explants with shoots, there was no significant effect for the factor BAP, increasing as the concentration of BAP grows. The variable number of shoots per explant showed interaction between factors, with the highest shoot formation in the presence of NAA and BAP in the range of 10 -15 µM. In the presence of auxin, the highest value occurred with 15 µM BAP, tending to decline regardless of the presence of NAA. The rooting of the apical segments was higher in the presence of NAA and absence of BAP, decreasing with increasing cytokinin. Leaf number was influenced by the higher concentration of BAP, and the amount of 15 µMhadthe largest number, tending to decrease with increasing concentration. The concentration of 15 µM BAP, regardless of NAA, provided better results in the multiplication of the species, producing increased number of shoots and leaves, except for rooting, whichwas influenced ...

show abstract

unclassified

Multiplicação in vitro de segmentos apicais caulinares de segurelha (Satureja hortensis L.)

Navroski

Waldow

Reiniger

et al. 2014

Rev. bras. plantas med.

View full text Add to dashboard Cite

show abstract

“…observed the highest percentage of friable calli formed came from leaf explants cultured in medium supplemented with NAA. In turn, Rodrigues and Almeida (2010), utilizing different concentrations of BAP, found that the addition of 26.64 μM of this cytokinin to the culture medium and the performance of two subcultures after the in vitro culture was necessary for callogenesis in Cissus sicyoides L. from leaf segments.…”

Section: Callus Oxidationmentioning

confidence: 99%

In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential

Rodrigues

Almeida²,

Lêdo

et al. 2020

Ciênc. agrotec.

View full text Add to dashboard Cite

Vernonia condensata Baker has been traditionally used in folk medicine for the treatment of several inflammatory and infectious processes. Overexploitation of this plant species has drastically reduced its population in its natural habitat (Cerrado). Therefore, tissue culture tools, such as somatic embryogenesis, can be used as an alternative method for rapid and large-scale plant regeneration. The objectives of this study were to induce callogenesis in Vernonia condensata from different types of explants and to evaluate the structural aspects of the development of pro-embryogenic masses of this species by means of histological analyses. The formation of calli was induced from leaf explants and internodal segments, which were inoculated in EME medium supplemented with 50 g L-1 sucrose, 0.5 g L-1 malt extract and 2.68 μM NAA, plus varying concentrations of BAP (0.00, 2.22, 4.44 or 8.88 μM). After 40 days, the following morphogenetic traits were evaluated: intensity of callus formation, intensity of oxidation, callus texture, and morphogenesis. The calli with embryogenic masses were analyzed by light and scanning electron microscopy. Both types of explants were responsive regarding callogenesis, with the BAP concentration of 4.44 μM promoting the formation of friable calli associated with a larger percentage of calli with embryogenic masses. Cells from leaf explants and internodal segments were able to dedifferentiate and change into embryonic structures.

show abstract

Calogênese em Cissus sicyoides L. a partir de segmentos foliares visando à produção de metabólitos in vitro

Cited by 2 publications

References 11 publications

Multiplicação in vitro de segmentos apicais caulinares de segurelha (Satureja hortensis L.)

Multiplicação in vitro de segmentos apicais caulinares de segurelha (Satureja hortensis L.)

In vitro callus induction and development of Vernonia condensata Baker with embryogenic potential

Contact Info

Product

Resources

About