Association between hepatic cholesterol and oleic acid in the liver of rats treated with partially hydrogenated vegetable oil

Abstract

OBJECTIVE: The aim of the present study was to investigate the lipid profiles of the hepatic and adipose tissues of Wistar rats treated for 21 days with a diet high in saturated fat (high saturated fat, n=6) or high in hydrogenated fat, that is, having 50% partially hydrogenated vegetable oil in its composition (high hydrogenated fat, n=6), and compare them to those of a control group (control group, n=6). METHODS: Adipose tissue and total hepatic fat were higher in the saturated fat group than in the hydrogenated fat group. Hepatic lipid peroxidation was greatest in the saturated fat group, with consequent lower hepatic vitamin E and A levels. In contrast, serum vitamin A was highest in the saturated fat group. Analysis of hepatic lipid fractions found more cholesterol and less high density lipoprotein-cholesterol in the hydrogenated fat group. The hydrogenated fat group had the highest levels of triacylglycerols, followed by the saturated fat group. RESULTS: Significant amounts of trans fatty acids were detected in the hepatic and adipose tissues of the hydrogenated fat group. Among the identified fatty acids, 18:1n9 had a higher positive association with hepatic cholesterol and triacylglycerols, and a higher negative association with high density lipoprotein-cholesterol. Partially hydrogenated vegetable oil promotes greater accumulation of cholesterol and triacylglycerols in the liver than saturated fats. CONCLUSION: Trans fatty acids were incorporated into hepatocytes and adipocytes in a highly efficient manner.

Jaboticaba (Myrciaria jaboticaba (Vell.) Berg.) peel improved triglycerides excretion and hepatic lipid peroxidation in high-fat-fed rats

“…Although the studied diets had the same fiber content, the differences may stem from the soluble fiber content of freeze-dried jaboticaba peel, from its polyphenol content, and/or from other yet unknown substances. Moreover, the lard added to the high-fat diets is high in saturated fatty acids, which could hinder lipid metabolism in rats 20 . Additionally, in the present study lard promoted obesity in SD rats.…”

Bioelectrical impedance analysis and anthropometry for the determination of body composition in rats: effects of high-fat and high-sucrose diets

“…Also, little is known about the validity of these methods for the anthropometric assessment of these animals. There are several experimental studies investigating the effects of high-fat and highsucrose diets on rats since these diets promote metabolic changes, but they usually only assess their effect on body weight [24][25][26] . BIA can accurately measure lean and fat mass and this distinction is important because excess body fat compromises health, and may promote the development of glucose intolerance and dyslipidemia.…”

“…The study included 24 The animals were allowed to adapt to the experimental conditions for one week, all of them receiving the standard AIN-93 diet. They were then divided randomly into 3 groups: Control group (C): group fed the standard diet AIN-93 (n=8); High-Fat group (HF): group fed a high-fat diet (n=8); and High-Sucrose group (HS): group fed a diet with a different type of carbohydrate (n=8).…”

<b>Coconut oil increases HDL-c and decreases triglycerides in wistar rats

“…(Castro, Almeida, Leonardi, Ovidio, & Jordão, 2012). The groups were treated orally (by gavage) with saline, soybean oil (9 kcal mL ) (according to the product label), purchased in supermarkets in Dourados, Mato Grosso do Sul State, Brazil.…”