AFLP marker analysis revealing genetic structure of the tree Parapiptadenia rigida (Benth.) Brenan (Leguminosae-Mimosoideae) in the southern Brazilian Tropical Rainforest

Abstract

Parapiptadenia rigida is a tropical early secondary succession tree characteristic of the Tropical Atlantic Rainforest. This species is of great ecological importance in the recovery of degraded areas. In this study we investigated the variability and population genetic structure of eight populations of P. rigida. Five AFLP primer combinations were used in a sample of 159 individuals representing these eight populations, rendering a total of 126 polymorphic fragments. The averages of percentage of polymorphic loci, gene diversity, and Shannon index were 60.45%, 0.217, and 0.322, respectively. A significant correlation between the population genetic variability and the population sizes was observed. The genetic variability within populations (72.20%) was higher than between these (22.80%). No perfect correlation was observed between geographic and genetic distances, which might be explained by differences in deforestation intensities that occurred in these areas. A dendrogram constructed by the UPGMA method revealed the formation of two clusters, these also confirmed by Bayesian analysis for the number of K cluster. These results show that it is necessary to develop urgent management strategies for the conservation of certain populations of P. rigida, while other populations still preserve reasonably high levels of genetic variability.

Pheno-morphological variation, genetic diversity and population structure of Tunisian Echinus Medic (Medicago ciliaris L.)

“…In addition, for clover species, this primer combination amplified 52 bands across four studied populations, which was the lowest number among the four primers used (Bennett and Mathews, 2003). In other studies, an average 25.2 markers generated per pairwise combinations (10 primers combinations) with a highest number of 28 markers was sufficient for analyses of genetic structure and diversity in eight populations of Parapiptadenia rigida (De Souza et al, 2013). Chennaoui-Kourda et al (2012) obtained 24-51 scorable bands using the AFLP technique to study the genetic diversity of Sulla, with an average of 36.8 bands per combination, and Marghali et al (2014) detected a total of 295 polymorphic bands.…”

Morphology and Genetic Diversity of Jabon Putih Seedling from 4 Populations in Sumatra, Nusa Kambangan, Kalimantan, and Sulawesi

“…Keragaman genetik dalam populasi yang relatif tinggi pada penelitian ini menunjukkan bahwa populasi jabon putih tersebut masih memiliki ukuran populasi yang cukup memadai untuk melakukan perkawinan silang sehingga keragamannya masih terpelihara. Hal serupa juga terjadi pada jenis Parapiptadenia rigida di Brazil Tenggara (Souza et al, 2013), Vatica mangachapoi di pulau Hainan (Zhang et al, 2012), dan Shorea robusta di India (Surabhi et al, 2017) yang menunjukkan keragaman dalam populasi yang tinggi. Keragaman ini terjadi disebabkan oleh sebagaian besar pohon sampel berada pada kawasan hutan sekunder yang memiliki populasi jabon putih cukup besar karena sifat pertumbuhan jabon putih sebagai pohon pioner yang secara umum mendominasi areal hutan sekunder (Sudrajat et al, 2014;Wahyuni & Kafiar, 2017;Aththorick et al, 2018).…”