Genetic and epigenetic effects of salinity on in vitro growth of barley

Demirkıran, Aykut; Marakli, Sevgi; Temel, Aslıhan; Gözükırmızı, Nermin

doi:10.1590/s1415-47572013000400016

Cited by 23 publications

(17 citation statements)

References 33 publications

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“…More recently, the RAPD assay has proven to be useful in investigating DNA damage and mutations within organisms under stress, toxicity, or irritation, etc. [24][25][26]28,48,49].…”

Section: Discussionmentioning

confidence: 99%

“…Coupled restriction enzyme digestion-random amplification (CRED-RA) is a method used to detect DNA methylation which is one of the main causes of epigenetic variations [28]. This technique allows amplification of a DNA fragment containing a cut site of a methylation sensitive restriction enzyme, only when the cytosine residue within the site is methylated and protected from digestion [50].…”

Section: Discussionmentioning

confidence: 99%

“…The amplification protocol began with a 5 min pre-denature step at 94°C, followed by 45 cycles of 1 min for denaturing at 94°C, 1 min for annealing at 36°C, and 2 min for extension at 72°C. After 45 cycles, a further 10 min step at 72°C was performed to allow completion of primer extension [26,28]. Amplification products were separated through gel electrophoresis in a 1.5% agarose gel in 1× TAE buffer with 100 V for 1 h. RAPD profiles of gDNA were visualized under a UV transilluminator.…”

Section: Rapd Analysismentioning

confidence: 99%

“…However, the ability of each member of this isoschizomer pair to cut DNA depends on cytosine methylation conditions specific to each enzyme. If one or both cytosines are methylated in a single strand (hemi-methylation), but not both DNA strands, HpaII does not cut the strand, whereas MspI does not cut if the outer cytosine is methylated in one or both strands [28,[30][31][32][33]. Therefore, the amplification bands of the enzyme-digested products can show both the presence of DNA methylation and types of DNA methylation occurring [28,[30][31][32][33].…”

Section: Cred-ra Analysismentioning

confidence: 99%

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EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.

Zhang

Shi

et al. 2016

Acta Soc Bot Pol

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In this study, when germinated Triticum aestivum L. seeds were treated with 0, 2, 4 and 6 mM ethyl glycol tetraacetic acid (EGTA), root growth was suppressed and the mitotic index decreased. These inhibitory effects were positively correlated with EGTA concentration. RT-PCR analysis revealed that the expression of several gene markers related to the G1/S transition of the cell cycle were significantly downregulated. Confocal microscopy of Fluo-3/AM-stained roots showed chelation of nearly all of the Ca 2+ within the root meristematic regions. Both random amplified polymorphic DNA (RAPD) and coupled restriction enzyme digestion-random amplification (CRED-RA) techniques showed significant increases in the levels of genomic DNA polymorphisms and degree of DNA methylation. The study provides information concerning the impact of Ca 2+ chelator, EGTA, on the growth, expression of cell cycle transition marker genes, and changes in DNA structure and methylation in the wheat roots.

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“…More recently, the RAPD assay has proven to be useful in investigating DNA damage and mutations within organisms under stress, toxicity, or irritation, etc. [24][25][26]28,48,49].…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Rapd Analysismentioning

confidence: 99%

Section: Cred-ra Analysismentioning

confidence: 99%

See 2 more Smart Citations

EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.

Zhang

Shi

et al. 2016

Acta Soc Bot Pol

View full text Add to dashboard Cite

show abstract

“…Salinity affects plant growth, activity of major cytosolic enzymes by disturbing intracellular potassium homeostasis, causing oxidative stress and programmed cell death, reduced nutrient uptake, metabolic toxicity, inhibition of photosynthesis, reduced CO2 assimilation and reduced root respiration (Sairam and Srivastava, 2002;Cuin and Shabala, 2007;Chen et al, 2007;Shabala, 2009;Abogadallah, 2010;Demirkiran et al, 2013;Liu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Salt Stress and Homobrassinosteroid Interactions during Germination in Barley Roots

Marakli

Temel

Gözükırmızı

2014

Not Bot Hort Agrobot Cluj

Self Cite

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Potential alleviation effects of Homobrassinosteroid (HBR) (0.5 and 1 µM HBR) on root germination, cell division and antioxidant system enzymes (superoxide dismutase and catalase) of barley (Hordeum vulgare L. cv. 'Hilal') roots grown under different salt concentrations (150 mM and 250 mM) were investigated during 48 and 72 h at dark with their controls. Salt applications decreased primary root lengths, seminal root lengths, number of roots from one seed, mitotic activity and induced mitotic abnormalities. In addition, salt application decreased protein content but increased enzyme activities both at 48 h and 72 h when compared to control. Roots treated with HBR enhanced root lengths and root number. HBR-treated roots showed more mitotic activity, mitotic abnormalities and significant enlargements at the root tips when compared to controls and only salt-treated samples. Salt + HBR applications stimulated root lengths and showed more mitotic activity and mitotic abnormalities when compared to only salt-treated samples. Salt application increased superoxide dismutase and catalase activities both at 48 h and 72 h. However, HBR application decreased total soluble protein content and increased enzyme activities especially at 48 h compared to control. Depending on concentration and timing, salt + HBR treatments showed varying results in total soluble protein content and enzyme activities. There was also a significant change in protein banding patterns of experimental groups separated by SDS-PAGE. These findings are expected to contribute to understanding of how HBR affect barley roots grown under different salt concentrations.

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Static magnetic field induced epigenetic changes in wheat callus

Aydın

Taspinar

Çakmak

et al. 2016

Bioelectromagnetics

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Deoxyribonucleic acid (DNA) is always damaged by endogenous and exogenous factors. Magnetic field (MF) is one of these exogenous factors. When repair mechanisms are not sufficient, mainly because of imbalance in damage or mistakes in repair mechanisms, methylation of DNA results in polymorphism-related abnormalities. In this study, low intensity static magnetic field-induced DNA damage and methylation in wheat calli were investigated by using Random Amplified Polymorphic DNA and Coupled Restriction Enzyme Digestion-Random Amplification techniques. Calli were derived from mature embryos of wheat. Both 7- and 14-day-old wheat calli were exposed to 7 mT (millitesla) static MF for 24, 48, 72, 96, or 120 h of incubation period. The highest change in polymorphism rate was obtained in calli exposed to 7 mT MF for 120 h in both 7- and 14-day-old calli. Increase in MF duration caused DNA hypermethylation in both 7- and 14-day-old calli. Polymorphism and DNA methylation ratio were higher in 7-day-old calli. The highest methylation level with a value of 25.1% was found in 7-day-old calli exposed to MF for 120 h. Results suggested that low intensity static magnetic field may trigger genomic instability and DNA methylation. Bioelectromagnetics. 37:504-511, 2016. © 2016 Wiley Periodicals, Inc.

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Genetic and epigenetic effects of salinity on in vitro growth of barley

Cited by 23 publications

References 33 publications

EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.

EGTA, a calcium chelator, affects cell cycle and increases DNA methylation in root tips of Triticum aestivum L.

Salt Stress and Homobrassinosteroid Interactions during Germination in Barley Roots

Static magnetic field induced epigenetic changes in wheat callus

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