Static magnetic field induced epigenetic changes in wheat callus

Abstract

Deoxyribonucleic acid (DNA) is always damaged by endogenous and exogenous factors. Magnetic field (MF) is one of these exogenous factors. When repair mechanisms are not sufficient, mainly because of imbalance in damage or mistakes in repair mechanisms, methylation of DNA results in polymorphism-related abnormalities. In this study, low intensity static magnetic field-induced DNA damage and methylation in wheat calli were investigated by using Random Amplified Polymorphic DNA and Coupled Restriction Enzyme Digestion-Random Amplification techniques. Calli were derived from mature embryos of wheat. Both 7- and 14-day-old wheat calli were exposed to 7 mT (millitesla) static MF for 24, 48, 72, 96, or 120 h of incubation period. The highest change in polymorphism rate was obtained in calli exposed to 7 mT MF for 120 h in both 7- and 14-day-old calli. Increase in MF duration caused DNA hypermethylation in both 7- and 14-day-old calli. Polymorphism and DNA methylation ratio were higher in 7-day-old calli. The highest methylation level with a value of 25.1% was found in 7-day-old calli exposed to MF for 120 h. Results suggested that low intensity static magnetic field may trigger genomic instability and DNA methylation. Bioelectromagnetics. 37:504-511, 2016. © 2016 Wiley Periodicals, Inc.

Сhiral and Racemic Fields Concept for Understanding of the Homochirality Origin, Asymmetric Catalysis, Chiral Superstructure Formation from Achiral Molecules, and B-Z DNA Conformational Transition

“…According to Ruiz-Gomez et al [325] the magnetic field effect on DNA is not certain. Aydin et al [326] believe that a low intensity static magnetic field may trigger genomic instability. "But this genotoxic effect of the magnetic field, however, is minimized in living organisms due to the presence of protectic cellular responses" [327] (see, however [328]).…”

Potential Impact of Geomagnetic Field in Transcranial Magnetic Stimulation for the Treatment of Neurodegenerative Diseases

“…Recent studies showed that 50 Hz power frequency magnetic fields produced remarkably altered methylation profile of genomic DNA in human neural cells (Giorgi et al, 2017 ) and micro RNAs-mediated deregulation of important signaling pathways in mouse spermatocyte-derived cells (Liu et al, 2015 ). Moreover, the low intensity (7 mT) of static magnetic fields increased DNA methylation and polymorphism in the callus of wheat embryos (Aydin et al, 2016 ). The results in developing cells suggest that alterations in the expression of micro RNAs, methylation and polymorphism in DNA, and cell signaling pathways, could be potential biomarkers for the assessment of EMF/GMF imprinting.…”

Biological Effects Due to Hypomagnetic Field and Its Research Progress

“…It is based on the research of static magnetic fields (SMFs). The interaction of SMFs with living organisms has early been become an important subfield [1] many studies on biological effects of SMFs have been carried out [2][3][4] and the first basic research is that the SMFs are classified as following four grades. (1) the weak grade (<1mT); (2) the moderate grade (1mT -1T); (3) the strong grade (1T-5T); and (4) the ultrastrong grade (>5T) [1].…”