Effects of physical exercise on butyrylcholinesterase in obese adolescents

Silva, Isabela M.W.; Leite, Neiva; Boberg, Dellyana Rodrigues; Chaves, Thais Januzzi; Eisfeld, G; Bono, Gleyse Freire; Souza, Ricardo Lehtonen Rodrigues de; Furtado-Alle, Lupe

doi:10.1590/s1415-47572012005000063

Cited by 9 publications

(5 citation statements)

References 11 publications

(15 reference statements)

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“…In this case, there were no significant reductions in the relative activity of G2 and G1-ALB between CDR1 and CDR3 patient groups (Table 3). These results differ from those of Boberg et al (2010) and Silva et al (2012) who reported a homogeneous reduction of all molecular forms, in a case-control study with obese and in a physical exercise intervention study with obese adolescents, respectively. Considering the different results found in the present study, it is possible that AD interferes in the regulation of proportionality between the relative activities of BChE molecular forms, which may further affect the role of this enzyme in the peripheral cholinergic system in AD progression.…”

Section: Discussioncontrasting

confidence: 99%

Butyrylcholinesterase: K variant, plasma activity, molecular forms and rivastigmine treatment in Alzheimer's disease in a Southern Brazilian population

Bono

Simão-Silva

Batistela

et al. 2015

Neurochemistry International

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Alzheimer's disease (AD) is a neurodegenerative disorder in which there is a decline of cholinergic function. The symptomatic AD treatment involves the use of ChEIs (cholinesterase inhibitors) as rivastigimine, a dual inhibitor. The human butyrylcholinesterase (BChE) is an enzyme that has specific roles in cholinergic neurotransmission and it has been associated with AD. In the serum, BChE is found in four main molecular forms: G1 (monomer); G1-ALB (monomer linked to albumin); G2 (dimer); and G4 (tetramer). The interaction between the products of BCHE gene and CHE2 locus results in CHE2 C5+ and CHE2 C5- phenotypes. CHE2 C5+ phenotype and BChE-K are factors that influence on BChE activity. This work aimed to verify the proportions of BChE molecular forms, total and relative activity in 139 AD patients and 139 elderly controls, taking into account K variant, CHE2 locus, rivastigmine treatment and clinical dementia rating (CDR) of AD patients. Phenotypic frequencies of CHE2 C5+ and frequency of the carriers of the K allele were similar between groups. Total BChE activity in plasma was significantly lower in AD patients than in elderly controls. Furthermore, we found that reduction on plasma BChE activity is associated directly with AD progression in AD patients and that rivastigmine treatment has a stronger effect on BChE activity within the CDR2 group. The reduction in BChE activity did not occur proportionally in all molecular forms. Multiple regression analysis results confirmed that AD acts as the main factor in plasma BChE activity reduction and that severe stages are related with an even greater reduction. These findings suggest that the reduction of total plasma BChE and relative BChE molecular forms activity in AD patients is probably associated with a feedback mechanism and provides a future perspective of using this enzyme as a possible plasmatic secondary marker for AD.

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Section: Discussioncontrasting

confidence: 99%

Butyrylcholinesterase: K variant, plasma activity, molecular forms and rivastigmine treatment in Alzheimer's disease in a Southern Brazilian population

Bono

Simão-Silva

Batistela

et al. 2015

Neurochemistry International

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“…It is probably a feedback system, therefore it is both the cause and the effect. This was suggested by Silva et al . (2012) , whose study showed the physiological regularization of BChE activity after a physical exercise program, where the BChE activity and lipid profile became normal in response to exercise.…”

Section: Discussionsupporting

confidence: 67%

“…Although it is able of hydrolyzing acetylcholine similar to AChE, BChE functions appear to be more varied and remain not fully understood ( Valle et al ., 2011 ). BChE activity is heritable (H 2 = 81.4 ± 2.8%, p = 1.0910 −32 ), influenced by BCHE gene polymorphisms ( Valle et al ., 2011 ), and associated with lipid metabolism and factors related to obesity, such as weight ( Chautard-Freire-Maia et al ., 1991 ), body mass index (BMI) ( Alcântara et al ., 2005 ; Valle et al ., 2011 ; Silva et al ., 2012 ; Lima et al ., 2013 ; Milano et al ., 2013 ) and lipid profile ( Alcântara et al ., 2002 ; Benyamin et al ., 2011 , Scacchi et al ., 2011 ; Chaves et al ., 2013 ; Lima et al ., 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women

et al. 2017

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Butyrylcholinesterase (BChE) activity and polymorphisms in its encoding gene had previously been associated with metabolic traits of obesity. This study investigated the association of three single nucleotide polymorphisms (SNPs) in the BCHE gene: -116G > A (rs1126680), 1615GA (rs1803274), 1914A < G (rs3495), with obesity and lipid metabolism markers, body mass index (BMI), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high density lipoprotein cholesterol (HDL-C), triglyceride (TG) levels, and BChE enzymatic activity in obese (BMI≥30/n = 226) and non-obese women (BMI < 25/n = 81). BCHE SNPs genotyping was obtained by TaqMan allelic discrimination assay and by RFLP-PCR. Plasmatic BChE activity was measured using propionylthiocholine as substrate. Similar allele frequencies were found in obese and non-obese women for the three studied SNPs (p > 0.05). The dominant and recessive models were tested, and different effects were found. The -116A allele showed a dominant effect in BChE activity reduction in both non-obese and obese women (p = 0.045 and p < 0.001, respectively). The 1914A > G and 1615GA SNPs influenced the TG levels only in obese women. The 1914G and the 1615A alleles were associated with decreased plasma levels of TG. Thus, our results suggest that the obesity condition, characterized by loss of energy homeostasis, is modulated by BCHE polymorphisms.

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“…Inhibition of AChE can increase the levels of acetylcholine, thereby providing symptomatic relief [ 45 ]. On the other hand, butyrylcholinesterase (BChE) is a serine hydrolase associates with lipid metabolism and indicators of metabolic syndrome such as body mass index, waist–hip ratio, waist circumference, weight, cholesterol, and triglyceride levels [ 46 – 48 ]. Increased BChE activity may also lead to a greater degradation of acetylcholine, which lowers the inhibitory effect on the production of cytokines [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolic fingerprinting, antioxidant characterization, and enzyme-inhibitory response of Monotheca buxifolia (Falc.) A. DC. extracts

Ali

Saleem

Mannan

et al. 2020

BMC Complement Med Ther

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Background Ethnobotanical and plant-based products allow for the isolation of active constituents against a number of maladies. Monotheca buxifolia is used by local communities due to its digestive and laxative properties, as well as its ability to cure liver, kidney, and urinary diseases. There is a need to explore the biological activities and chemical constituents of this medicinal plant. Methods In this work, the biochemical potential of M. buxifolia (Falc.) A. DC was explored and linked with its biological activities. Methanol and chloroform extracts from leaves and stems were investigated for total phenolic and flavonoid contents. Ultrahigh-performance liquid chromatography coupled with mass spectrometry (UHPLC–MS) was used to determine secondary-metabolite composition, while high-performance liquid chromatography coupled with photodiode array detection (HPLC–PDA) was used for polyphenolic quantification. In addition, we carried out in vitro assays to determine antioxidant potential and the enzyme-inhibitory response of M. buxifolia extracts. Results Phenolics (91 mg gallic-acid equivalent (GAE)/g) and flavonoids (48.86 mg quercetin equivalent (QE)/g) exhibited their highest concentration in the methanol extract of stems and the chloroform extract of leaves, respectively. UHPLC–MS analysis identified a number of important phytochemicals, belonging to the flavonoid, phenolic, alkaloid, and terpenoid classes of secondary metabolites. The methanol extract of leaves contained a diosgenin derivative and polygalacin D, while kaempferol and robinin were most abundant in the chloroform extract. The methanol extract of stems contained a greater peak area for diosgenin and kaempferol, whereas this was true for lucidumol A and 3-O-cis-coumaroyl maslinic acid in the chloroform extract. Rutin, epicatechin, and catechin were the main phenolics identified by HPLC–PDA analysis. The methanol extract of stems exhibited significant 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical-scavenging activities (145.18 and 279.04 mmol Trolox equivalent (TE)/g, respectively). The maximum cupric reducing antioxidant capacity (CUPRAC) (361.4 mg TE/g), ferric-reducing antioxidant power (FRAP) (247.19 mg TE/g), and total antioxidant potential (2.75 mmol TE/g) were depicted by the methanol extract of stems. The methanol extract of leaves exhibited stronger inhibition against acetylcholinesterase (AChE) and glucosidase, while the chloroform extract of stems was most active against butyrylcholinesterase (BChE) (4.27 mg galantamine equivalent (GALAE)/g). Similarly, the highest tyrosinase (140 mg kojic-acid equivalent (KAE)/g) and amylase (0.67 mmol acarbose equivalent (ACAE)/g) inhibition was observed for the methanol extract of stems. Conclusions UHPLC–MS analysis and HPLC–PDA quantification identified a number of bioactive secondary metabolites of M. buxifolia, which may be responsible for its antioxidant potential and enzyme-inhibitory response. M. buxifolia can be further explored for the isolation of its active components to be used as a drug.

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Effects of physical exercise on butyrylcholinesterase in obese adolescents

Cited by 9 publications

References 11 publications

Butyrylcholinesterase: K variant, plasma activity, molecular forms and rivastigmine treatment in Alzheimer's disease in a Southern Brazilian population

Butyrylcholinesterase: K variant, plasma activity, molecular forms and rivastigmine treatment in Alzheimer's disease in a Southern Brazilian population

Effect of BCHE single nucleotide polymorphisms on lipid metabolism markers in women

Metabolic fingerprinting, antioxidant characterization, and enzyme-inhibitory response of Monotheca buxifolia (Falc.) A. DC. extracts

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