The cytotoxic and growth inhibitory effects of palladium(II) complexes on MDA-MB-435 cells

Abstract

The antitumorigenic potential of two palladium(II) complexes, [Pd(ca2-o-phen)Cl2] – C1 and [Pd(dmba)(dppp)Cl] – C2, was evaluated, using MDA-MB-435 cells, a human breast adenocarcinoma cell-line that does not express the estrogen receptor α (ER−). Growth inhibition and induced alterations in cell-morphology were analyzed. The sulforhodamine B test showed that, compared to control cells, both C1 and C2 significantly inhibited (p < 0.5) cell growth. The maximum effect with both was achieved with 1 μM complexes, after 24 h of treatment. No further cell-growth inhibition was achieved by increasing concentration or incubation time. Cell morphology was analyzed after staining with hematoxylin-eosin (HE). The morphological changes noted in the treated cells were cell rounding-up, shrinkage, nuclear condensation and reduction of cell length (p < 0.05), thereby indicating that both C1 and C2 are cytotoxic to breast adenocarcinoma cells. All together, there was every indication that, by decreasing cell growth and inducing morphological changes, the tested complexes are cytotoxic, hence their potentiality as promising candidates for antineoplastic drug development.

Biological properties of metal complexes of curcumin

“…Results showed that the manganese complex generated a significant decrease in the IC 50 for all the tested malignant cell lines when compared to curcumin alone, especially for the HOS and the A375 cell lines . Due to the structural similarity between Pt 2+ and Pd 2+ complexes, many authors have investigated the potential anticancer activity of palladium 2+ complexes and have demonstrated that they are cytotoxic to tumor cells . Generally, the complexes of Pd 2+ showed lower antitumor activity than cisplatin due to their more chemically labile nature .…”

Procyanidin B2 cytotoxicity to MCF-7 human breast adenocarcinoma cells

“…Haematoxylin-eosin (HE) staining of cells for morphological studies was performed as previously reported[22], with modifications. After the incubation of cells plated on sterile microscope cover glass in Petri dishes, they were washed with 0.1 M PBS, pH 7.4, fixed with 70% acetone, dehydrated with ethanol series and HE staining was performed.…”

A New Series of Moisture Stable Metallacyclic Heteroleptic Titanium(IV) Complexes of 8-Hydroxyquinoline: Synthesis, Characterization, Stability Study and Cytotoxicity Evaluation

“…Moderate activity was observed for the remaining molecules. Complex 3h was identified as the highest tumour inhibiting molecule among all its derivatives and its cytotoxic potential was found to be 0.05 µM against 0.017 µM of a wellknown tumour inhibiting drug cisplatin [23]. The highest cytotoxic property of this molecule is due to its excellent symmetric arrangement [24].…”