Soybean genetic transformation: a valuable tool for the functional study of genes and the production of agronomically improved plants

Homrich, Milena Schenkel; Wiebke-Strohm, Beatriz; Weber, Ricardo Luís Mayer; Bodanese‐Zanettini, Maria Helena

doi:10.1590/s1415-47572012000600015

Cited by 61 publications

(49 citation statements)

References 152 publications

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“…Initially, research on biotech soybean focused on agronomic traits for securing yields. There has been great interest in the development of biotech crops with value-added traits to improve nutritional value and industrial applications (Homrich et al, 2012). Increasing interest in the production of functional crops has propelled the development of soybean crops with specific new nutrients and increased functionality.…”

Section: Introductionmentioning

confidence: 99%

Increased Production of α-Linolenic Acid in Soybean Seeds by Overexpression of Lesquerella FAD3-1

et al. 2020

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Soybean is a major crop that is used as a source of vegetable oil for human use. To develop transgenic soybean with high a-linolenic acid (ALA; 18:3) content, the FAD3-1 gene isolated from lesquerella (Physaria fendleri) was used to construct vectors with two different seed-specific promoters, soybean b-conglycinin (Pb-con) and kidney bean phaseolin (Pphas), and one constitutive cauliflower mosaic virus 35S promoter (P35S). The corresponding vectors were used for Agrobacterium-mediated transformation of imbibed mature half seeds. The transformation efficiency was approximately 2%, 1%, and 3% and 21, 7, and 17 transgenic plants were produced, respectively. T-DNA insertion and expression of the transgene were confirmed from most of the transgenic plants by polymerase chain reaction (PCR), quantitative real-time PCR (qPCR), reverse transcription PCR (RT-PCR), and Southern blot analysis. The fatty acid composition of soybean seeds was analyzed by gas chromatography. The 18:3 content in the transgenic generation T 1 seeds was increased 7-fold in Pb-con:PfFAD3-1, 4-fold in Pphas : PfFAD3-1, and 1.6-fold in P35S:PfFAD3-1 compared to the 18:3 content in soybean "Kwangankong". The increased content of 18:3 in the Pb-con:PfFAD3-1 soybean (T 1 ) resulted in a 52.6% increase in total fatty acids, with a larger decrease in 18:1 content than 18:2 content. The increase in 18:3 content was also maintained and reached 42% in the Pphas : PfFAD3-1 transgenic generation T 2 . Investigations of the agronomic traits of 12 Pb-con:PfFAD3-1 transgenic lines (T 1 ) revealed that plant height, number of branches, nodes, pods, total seeds, and total seed weight were significantly higher in several transgenic lines than those in non-transgenic soybean. Especially, an increase in seed size was observed upon expression of the PfFAD3-1 gene with the b-conglycinin promoter, and 6%-14% higher seed lengths were measured from the transgenic lines.

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Section: Introductionmentioning

confidence: 99%

Increased Production of α-Linolenic Acid in Soybean Seeds by Overexpression of Lesquerella FAD3-1

et al. 2020

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“…However, the constitutive overexpression of genes can affect plant development and metabolism (Hsieh et al , 2002; Homrich et al , 2012). Indeed, the control of the expression of drought-tolerant genes by stress-responsive and/or tissue-specific promoters has been used as alternative for the elimination of negative effect of constitutive gene expression driven by the CaMV35S promoter (Chan Ju et al , 2012; Banerjee et al , 2013; Yan et al , 2015).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a promoter responsive to salt, osmotic and dehydration stresses in soybean

et al. 2017

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Drought stress is the main limiting factor of soybean yield. Currently, genetic engineering has been one important tool in the development of drought-tolerant cultivars. A widely used strategy is the fusion of genes that confer tolerance under the control of the CaMV35S constitutive promoter; however, stress-responsive promoters would constitute the best alternative to the generation of drought-tolerant crops. We characterized the promoter of α-galactosidase soybean (GlymaGAL) gene that was previously identified as highly up-regulated by drought stress. The β-glucuronidase (GUS) activity of Arabidopsis transgenic plants bearing 1000- and 2000-bp fragments of the GlymaGAL promoter fused to the uidA gene was evaluated under air-dried, polyethylene glycol (PEG) and salt stress treatments. After 24 h of air-dried and PEG treatments, the pGAL-2kb led to an increase in GUS expression in leaf and root samples when compared to the control samples. These results were corroborated by qPCR expression analysis of the uidA gene. The pGAL-1kb showed no difference in GUS activity between control and treated samples. The pGAL-2kb promoter was evaluated in transgenic soybean roots, leading to an increase in EGFP expression under air-dried treatment. Our data indicates that pGAL-2kb could be a useful tool in developing drought-tolerant cultivars by driving gene expression.

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“…Among others, these results have proven the usefulness of RNAi-mediated resistance for crop improvement. Other cases of development of transgenic soybean have been reported by [44]. These authors show some scientific references where synthetic Bacillus thuringiensis cry genes were used to develop transgenic soybean and prevent agronomic loses caused by insects from Lepidoptera order such us Anticarsia gemmatalis, Pseudoplusia includens and Helicoverpa zea.…”

Section: Soybean (Glycine Max L)mentioning

confidence: 89%

Genetic Improvement of Oilseed Crops Using Modern Biotechnology

Villanueva-Mejía¹,

Álvarez²

2017

Advances in Seed Biology

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In 2009, big challenges facing the agricultural sector in the twenty-first century were presented to the world. Human population growth, increased life expectancy, loss of biodiversity, climate change and accelerated land degradation are the main factors contributing to rethink agriculture system production. In that scenery, modern biotechnology has set a stage for the advancement of agricultural practices and it is clearly an important ally to apply a broad array of technologies and innovative systems where they are most needed, such as enhancing crop productivity, increasing yields, and ultimately ensuring food security. One of the biggest challenges is related to technify production systems, but with no doubt, developing genetic improvement toward getting an efficient and sustainable agriculture, generating new seed qualities (new traits), such as, among others, to upset fatty acids content in oilseed crops have been growing up significantly due to industry interest. In this study, a review about the main advances in genetic improvement of some oilseed crops, starting with omics to understand metabolic routes and to find out key genes in seed oil production, and also, getting in use of modern biotechnology to alter the production of fatty acids, and to face biotic challenges in oilseed crops is presented.

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Soybean genetic transformation: a valuable tool for the functional study of genes and the production of agronomically improved plants

Cited by 61 publications

References 152 publications

Increased Production of α-Linolenic Acid in Soybean Seeds by Overexpression of Lesquerella FAD3-1

Increased Production of α-Linolenic Acid in Soybean Seeds by Overexpression of Lesquerella FAD3-1

Isolation and characterization of a promoter responsive to salt, osmotic and dehydration stresses in soybean

Genetic Improvement of Oilseed Crops Using Modern Biotechnology

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