Studies of micronuclei and other nuclear abnormalities in red blood cells of Colossoma macropomum exposed to methylmercury

Rocha, Carlos Alberto Machado da; Cunha, Lorena Araújo da; Pinheiro, Raul Henrique da Silva; Bahia, Marcelo de Oliveira; Burbano, Rommel Rodrı́guez

doi:10.1590/s1415-47572011000400024

Cited by 24 publications

(12 citation statements)

References 20 publications

(34 reference statements)

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“…The assay also specifically evaluates individual cells for the presence of NA which include binucleated cells, lobed, notched or blebbed (nuclear buds) nuclear membranes. The exact pathways leading to the induction of NA are less understood, but have also been used as indicators of exposure to mutagenic or genotoxic contaminants in numerous fish species [Baršiene et al, ; da Rocha et al, ]. A variety of chemicals such as PCBs, PAHs, heavy metals and pesticides [Al‐Sabt and Metcalfe, ; Bolognesi and Hayashi, ; Obiakor et al, ] and more recently CEC [Teles et al, ; Filby et al, ; Iturburu et al, ] have been linked to erythrocyte MN and NA induction in various fishes.…”

Section: Introductionmentioning

confidence: 99%

Micronuclei and other erythrocyte nuclear abnormalities in fishes from the Great Lakes Basin, USA

Braham

Blazer

Shaw

et al. 2017

Environ and Mol Mutagen

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Biological markers (biomarkers) sensitive to genotoxic and mutagenic contamination in fishes are widely used to identify exposure effects in aquatic environments. The micronucleus assay was incorporated into a suite of indicators to assess exposure to genotoxic and mutagenic contamination at five Great Lakes Areas of Concern (AOCs), as well as one non‐AOC (reference) site. The assay allowed enumeration of micronuclei as well as other nuclear abnormalities for both site and species comparisons. Erythrocyte abnormality data was also compared to skin and liver tumor prevalence and hepatic transcript abundance. Erythrocyte abnormalities were observed at all sites with variable occurrence and severity among sites and species. Benthic‐oriented brown bullhead (Ameiurus nebulosus) and white sucker (Catostomus commersonii) expressed lower rates of erythrocyte abnormalities, but higher rates of skin and liver neoplasms, when compared to pelagic‐oriented largemouth bass (Micropterus salmoides) or smallmouth bass (Micropterus dolomieu) at the same site. The reduced erythrocyte abnormalities, increased transcript abundance associated with Phase I and II toxicant responsive pathways, and increased neoplastic lesions among benthic‐oriented taxa may indicate the development of contaminant resistance of these species to more acute effects. Environ. Mol. Mutagen. 58:570–581, 2017. © 2017 This article is a U.S. Government work and is in the public domain in the USA. Environmental and Molecular Mutagenesis published by Wiley Periodicals, Inc. on behalf of Environmental Mutagen Society

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Section: Introductionmentioning

confidence: 99%

Micronuclei and other erythrocyte nuclear abnormalities in fishes from the Great Lakes Basin, USA

Braham

Blazer

Shaw

et al. 2017

Environ and Mol Mutagen

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“…Several studies have demonstrated the value of micronuclei as biomarkers and fish as sentinel organisms (e.g., Andrade et al 2004;Ferraro et al 2004;Souza and Fontanetti 2006). In addition, studies on genotoxicity indicate that C. macropomum has potential as an experimental model for monitoring aquatic ecosystems (Chapadense et al 2009;Groff et al 2010;Rocha et al 2011). The frequency of micronuclei and morphological nuclear abnormalities are effective tools for analyzing the health status of fish (Noga 2000;Webb and Gagnon 2002).…”

Section: Discussionmentioning

confidence: 99%

Genotoxic and hematological parameters in Colossoma macropomum (Pisces, Serrasalmidae) as biomarkers for environmental impact assessment in a protected area in northeastern Brazil

Neta

Sousa

Sobrinho

et al. 2015

Environ Sci Pollut Res

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Genotoxic and hematological parameters in tambaqui (Colossoma macropomum), a native freshwater fish, were used as biomarkers to assess exposure to environmental stressors within the Maracanã Protected Area of Maranhão State, Brazil. Fish were sampled at two sites-Serena Lagoon (control) and Ambude River-on four occasions (dry and rainy season), and biometric data (length and weight) recorded and blood collected from all fish for analysis. Erythrocyte indices-mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration-were calculated. Blood samples were stained with Giemsa and acridine orange, and microscopically examined for micronuclei and morphological nuclear abnormalities. Micronuclei were observed in fish from both sites, although the frequency was significantly higher in fish from the Ambude River and morphological nuclear abnormalities were only observed in fish from the Ambude River. More morphological nuclear abnormalities and a larger number of micronuclei were observed in erythrocytes stained with acridine orange compared with those stained with Giemsa. On average, erythrocyte indices were lower in fish from the Ambude River than from the Serena Lagoon. The results confirm that genotoxic and hematological parameters in C. macropomum can be used as indicators of environmental health and could be valuable tools for monitoring environmental conditions within protected areas.

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“…Moreover, Fish also used for the potential of mutagenic and carcinogenic study of pesticide contaminants present in aquatic [8][9][10][11].…”

Section:  Correspondence Addressmentioning

confidence: 99%

Assessing the Genotoxic Potentials of Methomyl-based Pesticide in Tilapia (Oreochromis niloticus) Using Micronucleus Assay

Islamy

Yanuhar

Hertika

2017

JELS

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Pesticides are recognized as serious pollutants in the aquatic environment with the potential to cause genotoxic on the aquatic organism, especially fish. The micronucleus (MN) assay has been used to evaluate genotoxicity of many compounds in polluted ecosystems such pesticides. im of this study to determine genotoxic effect of methomylbased pesticide on tilapia (Oreochromis niloticus). Fish were exposed to six different concentrations base on range finding test (0 ppm, 3.2 ppm, 4.2 ppm, 6.5 ppm, 8.7 ppm and 10 ppm) of methomyl-based pesticide. The micronucleus were collected from peripheral blood erythrocyte of fish after 96 h exposure. Peripheral blood samples smears were stained with Giemsa, MN frequencies were counted and statistically analyzed using one-way ANOVA. The result of this study showed after 96 hours exposed to methomyl-based pesticide, at concentration 0 ppm causes 0% mortality, at concentration to 3.2 ppm causes 30% mortality, at concentration 4.2 ppm causes 60% mortality, at concentration 6.5 ppm causes 70% mortality, at concentration 8.7 ppm causes 80% mortality, at concentration 10 ppm causes 100% mortality of fish test. Lethal Concentration 50 (LC50 -96 hours) of methomy-base pesticide towards tilapia (O. niloticus) is 4.015 ppm. Through micronuclei assay during 96 hour exposure of methomyl-based pesticide, the result shows that frequencies of micronuclei in erythrocyte of fish test at concentration at 0ppm is 12‰, 18‰ and 16‰; at concentration at 3.2ppm is 33‰, 26‰ and 29‰; at concentration at 4.2ppm is 41‰, 38‰ and 46‰; at concentration at 6.5ppm is 68‰, 81‰ and 82‰; at concentration 8.7ppm is 133‰, 130‰ and 137‰; at concentration 10ppm is 163‰, 166‰ and 156‰. It revealed that methomyl-based pesticide exposure induced after 96 h significantly (P<0.05) increased genotoxic potentials simultaneous with increased concentration.

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Studies of micronuclei and other nuclear abnormalities in red blood cells of Colossoma macropomum exposed to methylmercury

Cited by 24 publications

References 20 publications

Micronuclei and other erythrocyte nuclear abnormalities in fishes from the Great Lakes Basin, USA

Micronuclei and other erythrocyte nuclear abnormalities in fishes from the Great Lakes Basin, USA

Genotoxic and hematological parameters in Colossoma macropomum (Pisces, Serrasalmidae) as biomarkers for environmental impact assessment in a protected area in northeastern Brazil

Assessing the Genotoxic Potentials of Methomyl-based Pesticide in Tilapia (Oreochromis niloticus) Using Micronucleus Assay

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