Characterization of 13 microsatellite loci developed from Meconopsis horridula

Abstract

Meconopsis horridula is one of the eight most famous flowers in Chinese province of Yunnan. In this study, a modified biotin-streptavidin capture method was used to detect 13 microsatellite markers in the genome of M. horridula. The polymorphism of each locus was assessed in 24 samples collected from four populations. The number of alleles per locus ranged from 2 to 7 (mean: 3.2). The observed and expected heterozygosities ranged from 0.0833 to 0.9167 and 0.0816 to 0.8050, respectively. Additionally, nine of the 13 microsatellite markers were successfully amplified in three other congeneric species. These polymorphic SSR markers could be useful for studying the population genetics of M. horridula and for assessing genetic variation in this and congenerc species in conservation programs.

The Antitumor Activity of Meconopsis Horridula Hook, a Traditional Tibetan Medical Plant, in Murine Leukemia L1210 Cells

“…It is scattered on grassy or rocky slopes at altitudes of 3000-4900 m in southwestern China [10]. For hundreds of years, M. horridula has been used as a traditional Tibetan medicine to clear away heat, relieve pain, mobilize static blood, and to treat bruises [11].…”

Phytochemical and biological research of Papaver pharmaceutical resources

“…A modified biotin-streptavidin capture method was used to detect 13 microsatellite markers in the genome of M. horridula (Zhao et al, 2010). A modified biotin-streptavidin capture method was used to detect 13 microsatellite markers in the genome of M. horridula (Zhao et al, 2010).…”

Characterization of the complete chloroplast genome of the prickly blue poppy Meconopsis horridula Hook. f. & Thomson (Ranunculales: Papaveraceae)

“…f. & Thomson) is a perennial herb within the family Papaveraceae (order Ranunculales), and is mainly distributed in western China (incl. Gansu, Qinghai, Sichuan, Yunnan, and Tibet), Bhutan, northeastern India, northern Myanmar and Nepal with an elevation of 3600-5400 m (Zhang and Christopher 2008;Zhao Y et al 2010). As a traditional Tibetan medicine, this herb has been used to promote blood circulation, remove bruises and stasis, and relieve chest pain (Zhao F et al 2020), and may play a potential role in cancer treatment (Fan et al 2015;Tang et al 2020).…”