Isolation and characterization of genes functionally involved in ovarian development of the giant tiger shrimp Penaeus monodon by suppression subtractive hybridization (SSH)

Preechaphol, Rachanimuk; Klinbunga, Sirawut; Khamnamtong, Bavornlak; Menasveta, Piamsak

doi:10.1590/s1415-47572010000400014

Cited by 19 publications

(17 citation statements)

References 37 publications

(45 reference statements)

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“…Along with PGE 2, and PGF 2α , PGD 2 also has been identified in the rice field crab Oziothelphusa senex senex [13]. The presence of PGE 2 has been detected in the muscle and hemolymph of shrimp Penaeus monodon [14].…”

Section: Introductionmentioning

confidence: 99%

Role of Arachidonic Acid and COX Inhibitors in the Regulation of Reproduction in Freshwater Crab Oziothelphusa senex senex

Prameswari¹,

Hemalatha²,

Kishori³

et al. 2017

J Aquac Res Development

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Section: Introductionmentioning

confidence: 99%

Role of Arachidonic Acid and COX Inhibitors in the Regulation of Reproduction in Freshwater Crab Oziothelphusa senex senex

Prameswari¹,

Hemalatha²,

Kishori³

et al. 2017

J Aquac Res Development

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“…Prostaglandin D 2 (PGD 2 ), PGE 2 and PGF 2α have been detected in the fresh water field crab Oziotelphusa senex senex [20], while PGE 2 , thromboxane B2 (TXB 2 ) and 6-keto-PGF 1α have been reported in C. maenas [15]. In addition, PGE 2 has been identified in hemolymph, muscle and ovary of domesticated P. monodon [21], but the presence of PGF 2α has not been reported in this species.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, injection of PGE 2 and PGF 2α into ovaries of O. senex senex significantly increased the number and the diameter of the oocytes in a dose-dependent manner [20]. In domesticated P. monodon , the amounts of PGE 2 in ovaries and haemolymph increased along with developing ovary stages [21]. However, the correlation between prostaglandins and crustacean ovary development may be species specific, as the amounts of PGE 2 and PGF 2α were highest in ovaries stage I and continued to decrease until stage IV in M. japonicus [10].…”

Section: Introductionmentioning

confidence: 99%

Insights into the Prostanoid Pathway in the Ovary Development of the Penaeid Shrimp Penaeus monodon

et al. 2013

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The prostanoid pathway converts polyunsaturated fatty acids (PUFAs) into bioactive lipid mediators, including prostaglandins, thromboxanes and prostacyclins, all of which play vital roles in the immune and reproductive systems in most animal phyla. In crustaceans, PUFAs and prostaglandins have been detected and often associated with female reproductive maturation. However, the presence of prostanoid biosynthesis genes remained in question in these species. In this study, we outlined the prostanoid pathway in the black tiger shrimp Penaeus monodon based on the amplification of nine prostanoid biosynthesis genes: cytosolic phospholipase A2, hematopoietic prostaglandin D synthase, glutathione-dependent prostaglandin D synthase, prostaglandin E synthase 1, prostaglandin E synthase 2, prostaglandin E synthase 3, prostaglandin F synthase, thromboxane A synthase and cyclooxygenase. TBLASTX analysis confirmed the identities of these genes with 51-99% sequence identities to their closest homologs. In addition, prostaglandin F2α (PGF2α), which is a product of the prostaglandin F synthase enzyme, was detected for the first time in P. monodon ovaries along with the previously identified PUFAs and prostaglandin E2 (PGE2) using RP-HPLC and mass-spectrometry. The prostaglandin synthase activity was also observed in shrimp ovary homogenates using in vitro activity assay. When prostaglandin biosynthesis was examined in different stages of shrimp ovaries, we found that the amounts of prostaglandin F synthase gene transcripts and PGF2α decreased as the ovaries matured. These findings not only indicate the presence of a functional prostanoid pathway in penaeid shrimp, but also suggest a possible role of the PGF2α biosynthesis in shrimp ovarian development.

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“…Preechapho et al, isolated 109 known sequences and identified several reproduction-related transcripts in ovaries of the giant tiger shrimps (P. monodon) using SSH (Preechaphol, Klinbunga, Khamnamtongand, & Menasveta, 2010). Adisak et al, obtained 185 transcripts and uncovered 8 novel YHV-responsive genes from haemocyte libraries of P. monodon induced by yellow head virus after 24h and 48/72h (Prapavorarat, Pongsomboon, & Tassanakajon, 2010).…”

Section: Discussionmentioning

confidence: 99%

Profiling of Up-Regulated Genes Response to Acute Hypo-Osmotic Stress in Hepatopancreas and Gill of the Pacific White Shrimps (Litopenaeus vannamei)

Liu¹,

Sun²,

Dong³

et al. 2016

IJB

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Suppression subtractive hybridization (SSH) was applied to screen responsively up-regulation genes in hepatopancreas and gill of Litopenaeus vannamei induced by acute hypo-osmotic stress. In the hepatopancreas, 196 clones were randomly selected and sequenced. 131 non-redundant transcripts, corresponding to 41 genes, were found with elevated expressions. They were functionally clustered into eight biological processes which were protein synthesis and processing, carbohydrate metabolism and energy production, transport, cell growth, apoptosis, cytoskeletal component, cell defense and homeostasis, signal transduction, accounting for 26.2%, 14.3%, 16.7%, 4.7%, 4.7%, 2.3%, 11.9% and 7.1%, respectively. When confirmed by real time qRT-PCR, the gene expression levels of MnSOD, glutathione and glutamine synthetase showed significant increases (2.64-folds, 3.44-folds and 2.16-folds, respectively) in hepatopancreas (P<0.05). Totally 81 expressed sequence tags (ESTs) were obtained by random sequencing from the gill SSH cDNA library, and 52 unigenes, including 15 contigs and 37 singlets, after CP3 online matching were gained by clustering and assembling. The sequence alignment results revealed that 30 of them had significant homology to previously identified Genbank database sequences, while 22 unigenes did not match any sequence in the datebase and presumably represented unidentified cDNAs. Functionally, the unigenes were classified into six clusters, including ion channels and transport proteins (1); protein synthesis, translation and transcription factor (2); stress resistance and antioxidant factors (3); energy metabolism (4); signal receptor and transduction (5); cell fibrin and cytoskeletal proteins (6) with proportion of 23.3%, 20.0%, 20.0%, 16.7%, 10.0% and 10.0% respectively. Real time qRT-PCR confirmation revealed significant elevation of Arginine kinase, Carbonic anhydrase and NaK-ATPase-α-subunit expressions (3.73-folds, 2.55-folds and 5.83-folds, respectively) in the gill after acute low-osmotic stress (P < 0.05). These results provided insight into critical physiology processes and pathways constituting the hypo-osmotic stress adaption program in hepatopancreas and gill of L. vannamei.

show abstract

Isolation and characterization of genes functionally involved in ovarian development of the giant tiger shrimp Penaeus monodon by suppression subtractive hybridization (SSH)

Cited by 19 publications

References 37 publications

Role of Arachidonic Acid and COX Inhibitors in the Regulation of Reproduction in Freshwater Crab Oziothelphusa senex senex

Role of Arachidonic Acid and COX Inhibitors in the Regulation of Reproduction in Freshwater Crab Oziothelphusa senex senex

Insights into the Prostanoid Pathway in the Ovary Development of the Penaeid Shrimp Penaeus monodon

Profiling of Up-Regulated Genes Response to Acute Hypo-Osmotic Stress in Hepatopancreas and Gill of the Pacific White Shrimps (Litopenaeus vannamei)

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