Genetic characterization of 12 heterologous microsatellite markers for the giant tropical tree Cariniana legalis (Lecythidaceae)

Abstract

Twelve microsatellite loci previously developed in the tropical tree Cariniana estrellensis were genetically characterized in Cariniana legalis. Polymorphisms were assessed in 28 C. legalis individuals found between the Pardo and Mogi-Guaçu River basins in the state of São Paulo, Brazil. Of the 12 loci, 10 were polymorphic and exhibited Mendelian inheritance. The allelic richness at each locus ranged from 2-11, with an average of 7 alleles per locus, and the expected heterozygosity ranged from 0.07-0.88. These loci showed a high probability of paternity exclusion. The characteristics of these heterologous microsatellite markers indicate that they are suitable tools for investigating questions concerning population genetics in C. legalis.

Mendelian inheritance, genetic linkage, and genotypic disequilibrium for nine microsatellite loci in Cariniana estrellensis (Raddi) Kuntze (Lecythidaceae)

“…We found that the nine loci assessed herein present Mendelian segregation and are not linked. Thus, these molecular markers developed by Guidugli et al (2010) can be considered as genetic markers and our results support the hypothesis that they are not located in the same chromosome linkage group. However, genotypic disequilibrium was detected between four pairs of loci for adults.…”

“…Among these, genetic bottleneck and genetic drift can be the result of forest fragmentation due to decreases in effective population size, resulting in a limited number of pollen donors participating in reproduction. In studying a different population of the same species, Guidugli et al (2010) found no significant genotypic disequilibrium between the same pairwise loci assessed herein. Thus, the genotypic disequilibrium that we detected may be attributed to genetic drift caused by forest fragmentation in the study region.…”

“…However, in order to use molecular markers as genetic markers, it is important to determine whether their inheritance follows the rules of Mendelian segregation, and whether the loci are genetically linked (Tambarussi et al, 2013;Manoel et al, 2015;Moraes et al, 2016). Studies assessing linkage among loci are also necessary because the detected loci are used to calculate averages among loci in population genetic studies; therefore, linked loci can create bias in the estimates (Guidugli et al, 2010). To enable the analysis of genetic diversity and structure, mating system, and gene flow for C. estrellensis herein we assess the Mendelian inheritance, genetic linkage, and genotypic disequilibrium for nine microsatellite loci developed for the species.…”

Genetic diversity of Metrodorea nigra (Rutaceae) from a small forest remnant in Brazil assessed with microsatellite markers

“…Microsatellite markers or SSR (simple sequence repeats) have become powerful research tools because they are (mostly) co-dominant, abundant in genomes, highly reproducible, and some have a high ability to amplify orthologous loci in related species Nazareno et al, 2009;Guidugli et al, 2010). In this study, we developed microsatellite loci for M. nigra in order to facilitate detailed study of its genetic structure, gene flow and mating systems.…”

Cross transferability of SSRs to five species of Araucariaceae: a useful tool for population genetic studies in Araucaria araucana

“…Transferability of primers was attempted among species within different genus (e.g. Guidugli et al, 2010;Hendre et al, 2008;Heesacker et al, 2008;Zhang et al, 2007), families (e.g. Holmen et al, 2005;Brown et al, 2005;FitzSimmons et al, 1995), orders and classes (e.g.…”